LOCUS       sequence-210587-        7305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 275-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 275-972
FEATURES             Location/Qualifiers
     source          1..7305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1378..1399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1590..1609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1840..1857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1841..1857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1867..1886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1867..1885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4531..4551)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4531..4549)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4570..4586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4570..4586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4583..4605)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4594..4610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4618..4624,4625..4642,4643..4648))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(4801..4818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4972..5560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5052..5071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5731..6522,6523..6591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6354..6373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6592..6696)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    6733..7236
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     7278..7296
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgagcatgta
     1921 ctttccgcct aactaccatc acctgtcttt atttattccg ctgacagata tgcacaacca
     1981 attatcgtct cttgaaagct ttttagacgt gattgctgtt atttggcacg ttgttagtat
     2041 ttaagcttat tcgggtttgc attgatattt gcccgagcag aaatctcctt ctcgatgagt
     2101 tagaactgaa acatctcttc aattacttat acataataaa catagaaagc caggacctgt
     2161 catcatgcta aaaaccattc taacagtaat gagacctgat attgagaatg aggatgagta
     2221 tgttagaaat gtaacttcaa gagcggcggc tgtggtggca aaagcccttg gtgttaatca
     2281 attgcttcct ttcatcaatg cagcatgcca ctcacggaag tcttggaaag caaggcatac
     2341 tggcataaaa attgtgcagc aaataggtat acttttggga ataggtgtct tgaatcatct
     2401 tactgggctg atgagttgta tcaaagactg cctaatggat gatcacgttc cggtgcgaat
     2461 tgttacagca catactttat caacactagc agaaaattct tatccatatg gtatcgaagt
     2521 tttcaacgtt gtactggagc cattatggaa gggtataaga agtcatcgtg gtaaagtgct
     2581 atcttctttt ttaaaagctg ttggatctat gatcccattg atggacccag agtatgccgg
     2641 ttattatacc acagaagcaa tgagaataat tagacgtgaa ttcgactctc ccgatgatga
     2701 aatgaaaaag accattcttt tagtattaca aaaatgtagc gctgtagaat ccataacgcc
     2761 aaaatttctg agagaagaaa tcgcgccaga attctttcaa aaattttggg tcagacgagt
     2821 ggcattggat agaccattaa acaaagttgt tacatatact actgttacat tagcaaagaa
     2881 attaggttgt tcttatacca ttgataaatt attaacacca ttgagagatg aagctgagcc
     2941 gttcagaaca atggctgtcc atgctgttac tagaacagta aacttattag ggacagcaga
     3001 tttagatgag agattagaaa cgaggcttat tgatgcgctc ttgatagcat ttcaagaaca
     3061 aacaaatagt gattctataa tctttaaagg gtttggggca gtgacggtgt cacttgacat
     3121 ccgtatgaaa ccgtttttag cacccattgt gagtacgatt ttaaatcatt taaagcataa
     3181 aactcccttg gttcgtcaac atgcagcaga tctatgtgcc atactaatac cagtcatcaa
     3241 gaactgtcac gaattcgaaa tgttgaataa attgaacatt atattgtatg aatccttagg
     3301 cgaagtatat ccagaggtat taggttctat aatcaacgct atgtactgta ttactagcgt
     3361 aatggatttg gataaactac aacctcctat taatcaaatt ttaccaacct taactccaat
     3421 tttaagaaac aagcatagaa aagtcgaagt aaacactatc aaatttgttg gacttattgg
     3481 taaattagca cctacctatg cacctcctaa agaatggatg agaatatgtt ttgaactatt
     3541 agaacttttg aaaagtacaa ataaagaaat aagaagatca gcaaatgcca cgtttggatt
     3601 cattgctgaa gcaattggac ctcacgatgt tcttgttgcg ttattgaaca atttaaaggt
     3661 tcaagaacgt cagttacgtg tatgcacggc tgttgctatc ggtatcgttg ctaaagtgtg
     3721 tggtccttac aacgtgctgc cagtaatcat gaatgaatac acaacaccag agactaatgt
     3781 tcaaaacggt gttctcaagg ccatgtcatt catgtttgag tacattggta acatgtctaa
     3841 ggattacata tacttcataa caccattatt agaagatgca ctcaccgata gggatttagt
     3901 tcatcgtcag acagcgtcga acgtaataac ccacttagct ttaaactgtt caggtacagg
     3961 tcacgaagat gccttcattc atttaatgaa tcttctaata cccaatattt ttgaaacttc
     4021 accacatgct attatgcgta ttttagaagg tttggaggca ctgagtcaag ctcttggccc
     4081 tggactattc atgaactaca tatgggcagg attgttccat ccagcgaaga atgtaaggaa
     4141 agcattttgg agggtgtata ataacatgta cgtaatgtac caggacgcca tggtaccttt
     4201 ttaccccgtt acaccagaca acaatgaaga atatatagaa gaactggatt tagttctgtg
     4261 aatatatata gagcatataa ataaccacaa cttgtaagaa tattttactt gacacaccta
     4321 tatcatcacg tcacgatgcc ttcatagatt atgatgaaat ataaacagaa cctgctattg
     4381 gaaaaaaaag ggcaggaaaa aattttgtga aagtgagtaa aagaaagatg aagagcgaga
     4441 catggataat aagaaataga tgtcactaat ggacaattgg aagactgata tggaaagtta
     4501 cgatgaagga ggagctccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     4561 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4621 cataggagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga ggtaactcac
     4681 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4741 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4921 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5221 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5281 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5341 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5401 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5461 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5521 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5581 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5641 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5701 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5761 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5821 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5881 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5941 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6001 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6061 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6121 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6181 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6241 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6301 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6361 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6421 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6481 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6541 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6601 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6661 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6721 tgggtccttt tcatcacgtg ctataaaaat aattataatt taaatttttt aatataaata
     6781 tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt ttgttttccg
     6841 aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct tgctcttcct
     6901 gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaagacc acacacgaaa
     6961 atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt aatctgcttt
     7021 tcttgtctaa taaatatata tgtaaagtac gctttttgtt gaaatttttt aaacctttgt
     7081 ttattttttt ttcttcattc cgtaactctt ctaccttctt tatttacttt ctaaaatcca
     7141 aatacaaaac ataaaaataa ataaacacag agtaaattcc caaattattc catcattaaa
     7201 agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc ctaagaaacc attattatca
     7261 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
