LOCUS       sequence-210589-        7200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 310-972).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 310-972
FEATURES             Location/Qualifiers
     source          1..7200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1378..1399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1590..1609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1840..1857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1841..1857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1867..1886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1867..1885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4426..4446)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4426..4444)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4465..4481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4465..4481)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4478..4500)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4489..4505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4513..4519,4520..4537,4538..4543))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(4696..4713)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4867..5455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4947..4966)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5626..6417,6418..6486))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6249..6268
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6487..6591)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    6628..7131
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     7173..7191
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgagcatgta
     1921 ctttccgcct aactaccatc acctgtcttt atttattccg ctgacagata tgcacaacca
     1981 attatcgtct cttgaaagct ttttagacgt gattgctgtt atttggcacg ttgttagtat
     2041 ttaagcttat tcgggtttgc attgatattt gcccgagcag aaatctcctt ctcgatgagt
     2101 tagaactgaa acatctcttc aattacttat acataataaa catagaaagc caggacctgt
     2161 catcatggtt aatcaattgc ttcctttcat caatgcagca tgccactcac ggaagtcttg
     2221 gaaagcaagg catactggca taaaaattgt gcagcaaata ggtatacttt tgggaatagg
     2281 tgtcttgaat catcttactg ggctgatgag ttgtatcaaa gactgcctaa tggatgatca
     2341 cgttccggtg cgaattgtta cagcacatac tttatcaaca ctagcagaaa attcttatcc
     2401 atatggtatc gaagttttca acgttgtact ggagccatta tggaagggta taagaagtca
     2461 tcgtggtaaa gtgctatctt cttttttaaa agctgttgga tctatgatcc cattgatgga
     2521 cccagagtat gccggttatt ataccacaga agcaatgaga ataattagac gtgaattcga
     2581 ctctcccgat gatgaaatga aaaagaccat tcttttagta ttacaaaaat gtagcgctgt
     2641 agaatccata acgccaaaat ttctgagaga agaaatcgcg ccagaattct ttcaaaaatt
     2701 ttgggtcaga cgagtggcat tggatagacc attaaacaaa gttgttacat atactactgt
     2761 tacattagca aagaaattag gttgttctta taccattgat aaattattaa caccattgag
     2821 agatgaagct gagccgttca gaacaatggc tgtccatgct gttactagaa cagtaaactt
     2881 attagggaca gcagatttag atgagagatt agaaacgagg cttattgatg cgctcttgat
     2941 agcatttcaa gaacaaacaa atagtgattc tataatcttt aaagggtttg gggcagtgac
     3001 ggtgtcactt gacatccgta tgaaaccgtt tttagcaccc attgtgagta cgattttaaa
     3061 tcatttaaag cataaaactc ccttggttcg tcaacatgca gcagatctat gtgccatact
     3121 aataccagtc atcaagaact gtcacgaatt cgaaatgttg aataaattga acattatatt
     3181 gtatgaatcc ttaggcgaag tatatccaga ggtattaggt tctataatca acgctatgta
     3241 ctgtattact agcgtaatgg atttggataa actacaacct cctattaatc aaattttacc
     3301 aaccttaact ccaattttaa gaaacaagca tagaaaagtc gaagtaaaca ctatcaaatt
     3361 tgttggactt attggtaaat tagcacctac ctatgcacct cctaaagaat ggatgagaat
     3421 atgttttgaa ctattagaac ttttgaaaag tacaaataaa gaaataagaa gatcagcaaa
     3481 tgccacgttt ggattcattg ctgaagcaat tggacctcac gatgttcttg ttgcgttatt
     3541 gaacaattta aaggttcaag aacgtcagtt acgtgtatgc acggctgttg ctatcggtat
     3601 cgttgctaaa gtgtgtggtc cttacaacgt gctgccagta atcatgaatg aatacacaac
     3661 accagagact aatgttcaaa acggtgttct caaggccatg tcattcatgt ttgagtacat
     3721 tggtaacatg tctaaggatt acatatactt cataacacca ttattagaag atgcactcac
     3781 cgatagggat ttagttcatc gtcagacagc gtcgaacgta ataacccact tagctttaaa
     3841 ctgttcaggt acaggtcacg aagatgcctt cattcattta atgaatcttc taatacccaa
     3901 tatttttgaa acttcaccac atgctattat gcgtatttta gaaggtttgg aggcactgag
     3961 tcaagctctt ggccctggac tattcatgaa ctacatatgg gcaggattgt tccatccagc
     4021 gaagaatgta aggaaagcat tttggagggt gtataataac atgtacgtaa tgtaccagga
     4081 cgccatggta cctttttacc ccgttacacc agacaacaat gaagaatata tagaagaact
     4141 ggatttagtt ctgtgaatat atatagagca tataaataac cacaacttgt aagaatattt
     4201 tacttgacac acctatatca tcacgtcacg atgccttcat agattatgat gaaatataaa
     4261 cagaacctgc tattggaaaa aaaagggcag gaaaaaattt tgtgaaagtg agtaaaagaa
     4321 agatgaagag cgagacatgg ataataagaa atagatgtca ctaatggaca attggaagac
     4381 tgatatggaa agttacgatg aaggaggagc tcccagcttt tgttcccttt agtgagggtt
     4441 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4501 cacaattcca cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4561 agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4621 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4681 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4741 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4801 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4861 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4921 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4981 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5041 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5101 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5161 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5221 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5281 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     5341 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5401 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5461 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5521 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5581 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5641 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5701 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5761 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5821 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5881 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5941 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6001 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6061 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6121 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6181 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6241 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6301 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6361 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6421 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6481 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6541 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6601 ccgaaaagtg ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat
     6661 tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat
     6721 agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt
     6781 tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga
     6841 agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc
     6901 tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat
     6961 tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt
     7021 actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat
     7081 tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtcctaag
     7141 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
