LOCUS       sequence-21059-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG424GPD-ccdB-Cerulean
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(25..149)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(157..173)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(157..173)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(179..442)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(262..285)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 cctaggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
       61 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
      121 tctcgttcag cttttttgta caaacttgtg atgggggatc cactagttct agaatccgtc
      181 gaaactaagt tctggtgttt taaaactaaa aaaaagacta actataaaag tagaatttaa
      241 gaagtttaag aaatagattt acagaattac aatcaatacc taccgtcttt atatacttat
      301 tagtcaagta ggggaataat ttcagggaac tggtttcaac cttttttttc agctttttcc
      361 aaatcagaga gagcagaagg taatagaagg tgtaagaaaa tgagatagat acatgcgtgg
      421 gtcaattgcc ttgtgtcatc atttactcca ggcaggttgc atcactccat tgaggttgtg
      481 cccgtttttt gcctgtttgt gcccctgttc tctgtagttg cgctaagaga atggacctat
      541 gaactgatgg ttggtgaaga aaacaatatt ttggtgctgg gattcttttt ttttctggat
      601 gccagcttaa aaagcgggct ccattatatt tagtggatgc caggaataaa ctgttcaccc
      661 agacacctac gatgttatat att
//
