LOCUS       sequence-210599-        8046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated Hsh155 (aa 1-945).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Carrocci TJ, Paulson JC, Hoskins AA
  TITLE     Functional Analysis of Hsh155/SF3b1 Interactions with the U2 
            snRNA/Branch Site Duplex.
  JOURNAL   RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118.
  PUBMED    29752352
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2018 
            May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118."
COMMENT     Sequence Label: Hsh155 1-945
FEATURES             Location/Qualifiers
     source          1..8046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1378..1399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1590..1609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1840..1857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1841..1857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1867..1886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1867..1885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5272..5292)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5272..5290)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5311..5327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5311..5327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5324..5346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5335..5351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5359..5365,5366..5383,5384..5389))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5542..5559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5713..6301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5793..5812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6472..7263,7264..7332))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7095..7114
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7333..7437)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7474..7977
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8019..8037
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgagcatgta
     1921 ctttccgcct aactaccatc acctgtcttt atttattccg ctgacagata tgcacaacca
     1981 attatcgtct cttgaaagct ttttagacgt gattgctgtt atttggcacg ttgttagtat
     2041 ttaagcttat tcgggtttgc attgatattt gcccgagcag aaatctcctt ctcgatgagt
     2101 tagaactgaa acatctcttc aattacttat acataataaa catagaaagc caggacctgt
     2161 catcatgagt catccgattc aatttgtaaa cgctaataat agcgataaga gccaccaatt
     2221 aggcggtcaa tattctattc ctcaggattt gagggaaaac ctgcagaaag aagctgcacg
     2281 tattggtgag aacgaaaaag atgtgctaca ggaaaaaatg gaaactagga cagtgcaaaa
     2341 tcgagaggat tcataccaca aaagaaggtt tgatatgaag tttgagccgg attccgatac
     2401 acaaactgtt acgtcttcag aaaataccca agacgctgtc gtgccaagga aaagaaaatc
     2461 tcgttgggat gtaaaagggt acgagccacc tgatgaatct tcaacagcgg tcaaagaaaa
     2521 ctctgatagt gctttggtta atgtagaagg aatccatgat ttgatgtttt ttaaaccttc
     2581 tgaccataaa tatttcgccg acgttatatc caaaaaaccc atagatgagt taaataagga
     2641 tgaaaagaaa gaaagaaccc tgtcgatgtt acttctgaaa ataaaaaatg gcaatacagc
     2701 gagtaggagg acttctatgc ggattttaac cgataaagct gttacatttg gtccagaaat
     2761 gatatttaac cgcttattgc ccatattact agacagaagt ttggaagacc aggaaagaca
     2821 tctaatgatc aagaccatag atcgcgtcct ttaccagcta ggagatttaa caaaacctta
     2881 cgttcacaaa atacttgtag tagctgcccc attgctaata gatgaagatc caatggttcg
     2941 ttcaacaggc caggagataa tcaccaatct gtctacagtc gctggcctaa aaaccattct
     3001 aacagtaatg agacctgata ttgagaatga ggatgagtat gttagaaatg taacttcaag
     3061 agcggcggct gtggtggcaa aagcccttgg tgttaatcaa ttgcttcctt tcatcaatgc
     3121 agcatgccac tcacggaagt cttggaaagc aaggcatact ggcataaaaa ttgtgcagca
     3181 aataggtata cttttgggaa taggtgtctt gaatcatctt actgggctga tgagttgtat
     3241 caaagactgc ctaatggatg atcacgttcc ggtgcgaatt gttacagcac atactttatc
     3301 aacactagca gaaaattctt atccatatgg tatcgaagtt ttcaacgttg tactggagcc
     3361 attatggaag ggtataagaa gtcatcgtgg taaagtgcta tcttcttttt taaaagctgt
     3421 tggatctatg atcccattga tggacccaga gtatgccggt tattatacca cagaagcaat
     3481 gagaataatt agacgtgaat tcgactctcc cgatgatgaa atgaaaaaga ccattctttt
     3541 agtattacaa aaatgtagcg ctgtagaatc cataacgcca aaatttctga gagaagaaat
     3601 cgcgccagaa ttctttcaaa aattttgggt cagacgagtg gcattggata gaccattaaa
     3661 caaagttgtt acatatacta ctgttacatt agcaaagaaa ttaggttgtt cttataccat
     3721 tgataaatta ttaacaccat tgagagatga agctgagccg ttcagaacaa tggctgtcca
     3781 tgctgttact agaacagtaa acttattagg gacagcagat ttagatgaga gattagaaac
     3841 gaggcttatt gatgcgctct tgatagcatt tcaagaacaa acaaatagtg attctataat
     3901 ctttaaaggg tttggggcag tgacggtgtc acttgacatc cgtatgaaac cgtttttagc
     3961 acccattgtg agtacgattt taaatcattt aaagcataaa actcccttgg ttcgtcaaca
     4021 tgcagcagat ctatgtgcca tactaatacc agtcatcaag aactgtcacg aattcgaaat
     4081 gttgaataaa ttgaacatta tattgtatga atccttaggc gaagtatatc cagaggtatt
     4141 aggttctata atcaacgcta tgtactgtat tactagcgta atggatttgg ataaactaca
     4201 acctcctatt aatcaaattt taccaacctt aactccaatt ttaagaaaca agcatagaaa
     4261 agtcgaagta aacactatca aatttgttgg acttattggt aaattagcac ctacctatgc
     4321 acctcctaaa gaatggatga gaatatgttt tgaactatta gaacttttga aaagtacaaa
     4381 taaagaaata agaagatcag caaatgccac gtttggattc attgctgaag caattggacc
     4441 tcacgatgtt cttgttgcgt tattgaacaa tttaaaggtt caagaacgtc agttacgtgt
     4501 atgcacggct gttgctatcg gtatcgttgc taaagtgtgt ggtccttaca acgtgctgcc
     4561 agtaatcatg aatgaataca caacaccaga gactaatgtt caaaacggtg ttctcaaggc
     4621 catgtcattc atgtttgagt acattggtaa catgtctaag gattacatat acttcataac
     4681 accattatta gaagatgcac tcaccgatag ggatttagtt catcgtcaga cagcgtcgaa
     4741 cgtaataacc cacttagctt taaactgttc aggtacaggt cacgaagatg ccttcattca
     4801 tttaatgaat cttctaatac ccaatatttt tgaaacttca ccacatgcta ttatgcgtat
     4861 tttagaaggt ttggaggcac tgagtcaagc tcttggccct ggactattca tgaactacat
     4921 atgggcagga ttgttccatc cagcgaagaa tgtaaggaaa gcattttgga gggtgtataa
     4981 taacatgtac gtaatgtact gaatatatat agagcatata aataaccaca acttgtaaga
     5041 atattttact tgacacacct atatcatcac gtcacgatgc cttcatagat tatgatgaaa
     5101 tataaacaga acctgctatt ggaaaaaaaa gggcaggaaa aaattttgtg aaagtgagta
     5161 aaagaaagat gaagagcgag acatggataa taagaaatag atgtcactaa tggacaattg
     5221 gaagactgat atggaaagtt acgatgaagg aggagctccc agcttttgtt ccctttagtg
     5281 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5341 tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag cctggggtgc
     5401 ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5461 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5521 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5581 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5641 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5701 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5761 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5821 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5881 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5941 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6001 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6061 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6121 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6181 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6241 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6301 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6361 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6421 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6481 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6541 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6601 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6661 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6721 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6781 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6841 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6901 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6961 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7021 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7081 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7141 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7201 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7261 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7321 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7381 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7441 atttccccga aaagtgccac ctgggtcctt ttcatcacgt gctataaaaa taattataat
     7501 ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga
     7561 aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact
     7621 accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg
     7681 tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg
     7741 tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt
     7801 tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct
     7861 ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc
     7921 ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatccgt
     7981 cctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     8041 ttcgtc
//
