LOCUS       sequence-21060-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG424GPD-ccdB-Cerulean
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(89..213)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(290..311)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(551..570)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
       61 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
      121 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      181 cgtttctcgt tcagctttct tgtacaaagt ggtgatgggc tgcaggaatt cgatatcaag
      241 cttaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      301 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      361 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      421 caccctcgtg accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat
      481 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      541 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      601 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      661 gca
//
