LOCUS       sequence-21062-e         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GAL-ccdB-Cerulean
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(35..159)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(167..183)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(167..183)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(198..639)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(204..227)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    522..639
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 aagtgtaaag cctaggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt
       61 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
      121 attttacgtt tctcgttcag cttttttgta caaacttgtg atgggggatc cactagttct
      181 agaatccggg gttttttctc cttgacgtta aagtatagag gtatattaac aattttttgt
      241 tgatactttt attacatttg aataagaagt aatacaaacc gaaaatgttg aaagtattag
      301 ttaaagtggt tatgcagttt ttgcatttat atatctgtta atagatcaaa aatcatcgct
      361 tcgctgatta attaccccag aaataaggct aaaaaactaa tcgcattatc atcctatggt
      421 tgttaatttg attcgttcat ttgaaggttt gtggggccag gttactgcca atttttcctc
      481 ttcataacca taaaagctag tattgtagaa tctttattgt tcggagcagt gcggcgcgag
      541 gcacatctgc gtttcaggaa cgcgaccggt gaagacgagg acgcacggag gagagtcttc
      601 cttcggaggg ctgtcacccg ctcggcggct tctaatccgt actagagctc cagcttttgt
      661 tccctttagt gaggg
//
