LOCUS       sequence-210620-        6078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 400 / 490, Emission: 468 / 
            511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6078)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..6078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             671..1384
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    2924..3045
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2961..2980)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3015..3034
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3052..3507)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3189..3210)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3401..3420
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3534..3638
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3640..3997
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3640..3831
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3663..3683)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3848..3983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4032..4826
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4032..4826
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4086..4105)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4696..4715
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5014..5033)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5058..5105
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5434..6022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5923..5942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggtcgccacc atgagcaagg gcgccgagct gttcaccggc atcgtgccca tcctgatcga
      721 gctgaatggc gatgtgaatg gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc
      781 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg
      841 gcccaccctg gtggccaccc tgagctacgg cgtgcagtgc ttctcacgct accccgatca
      901 catgaagcag cacgacttct tcaagagcgc catgcctgag ggctacatcc aggagcgcac
      961 catcttcttc gaggatgacg gcaactacaa gacgcgcgcc gaggtgaagt tcgagggcga
     1021 taccctggtg agtcgcatcg agctgaccgg cactgatttc aaggaggatg gcaacatcct
     1081 gggcaataag atggagtaca actacaacgc caccaatgtg tacatcgtgg ccgacaaggc
     1141 caggaatggc atcaaggtga acttcaaggt ccgccacaac atcaaggatg gcagcgtgca
     1201 gctggccgac cactaccagc agaatacccc catcggcgat ggccctgtgc tgctgcccga
     1261 taaccactac ctgtccaccc agagcgccct gtccaaggac cccaacgaga agcgcgatca
     1321 catgatctac ctcgagttcg tgaccgccgc cgccatcacc cacggcatgg atgagctgta
     1381 caagtccgga ctcagatctg gcagcggtgg aggcagcgca tccggcggaa gcggaagcgt
     1441 gcgtgagtgc atctccatcc acgttggcca ggctggtgtc cagattggca atgcctgctg
     1501 ggagctctac tgcctggaac acggcatcca gcccgatggc cagatgccaa gtgacaagac
     1561 cattggggga ggagatgact ccttcaacac cttcttcagt gagacgggcg ctggcaagca
     1621 cgtgccccgg gctgtgtttg tagacttgga acccacagtc attgatgaag ttcgcactgg
     1681 cacctaccgc cagctcttcc accctgagca gctcatcaca ggcaaggaag atgctgccaa
     1741 taactatgcc cgagggcact acaccattgg caaggagatc attgaccttg tgttggaccg
     1801 aattcgcaag ctggctgacc agtgcaccgg tcttcagggc ttcttggttt tccacagctt
     1861 tggtggggga actggttctg ggttcacctc cctgctcatg gaacgtctct cagttgatta
     1921 tggcaagaag tccaagctgg agttctccat ttacccagca ccccaggttt ccacagctgt
     1981 agttgagccc tacaactcca tcctcaccac ccacaccacc ctggagcact ctgattgtgc
     2041 cttcatggta gacaatgagg ccatctatga catctgtcgt agaaacctcg atatcgagcg
     2101 cccaacctac actaacctta accgccttat tagccagatt gtgtcctcca tcactgcttc
     2161 cctgagattt gatggagccc tgaatgttga cctgacagaa ttccagacca acctggtgcc
     2221 ctacccccgc atccacttcc ctctggccac atatgcccct gtcatctctg ctgagaaagc
     2281 ctaccatgaa cagctttctg tagcagagat caccaatgct tgctttgagc cagccaacca
     2341 gatggtgaaa tgtgaccctc gccatggtaa atacatggct tgctgcctgt tgtaccgtgg
     2401 tgacgtggtt cccaaagatg tcaatgctgc cattgccacc atcaaaacca agcgcagcat
     2461 ccagtttgtg gattggtgcc ccactggctt caaggttggc atcaactacc agcctcccac
     2521 tgtggtgcct ggtggagacc tggccaaggt acagagagct gtgtgcatgc tgagcaacac
     2581 cacagccatt gctgaggcct gggctcgcct ggaccacaag tttgacctga tgtatgccaa
     2641 gcgtgccttt gttcactggt acgtgggtga ggggatggag gaaggcgagt tttcagaggc
     2701 ccgtgaagat atggctgccc ttgagaagga ttatgaggag gttggtgtgg attctgttga
     2761 aggagagggt gaggaagaag gagaggaata ctaaggatcc accggatcta gataactgat
     2821 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2881 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     2941 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3001 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     3061 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     3121 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     3181 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     3241 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     3301 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     3361 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     3421 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3481 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3541 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3601 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     3661 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3721 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3781 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3841 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3901 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3961 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     4021 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4081 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4141 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     4201 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     4261 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4321 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4381 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4441 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4501 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     4561 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4621 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     4681 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     4741 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     4801 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     4861 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     4921 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     4981 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     5041 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     5101 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     5161 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     5221 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     5281 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5341 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5401 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5581 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5821 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6061 ttgctggcct tttgctca
//
