LOCUS       sequence-210622-        6042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 400 / 490, Emission: 468 / 
            511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6042)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Cx43-7
FEATURES             Location/Qualifiers
     source          1..6042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2049..2765
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    2888..3009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2925..2944)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2979..2998
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3016..3471)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3153..3174)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3365..3384
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3498..3602
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3604..3961
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3604..3795
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3627..3647)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3812..3947
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3996..4790
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3996..4790
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4050..4069)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4660..4679
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4978..4997)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5022..5069
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5398..5986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5887..5906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctcgagctca agcttcgaat tcggcagcac ttttctttca ttggggggaa
      721 ggcgtgagga aagtaccaaa cagcagcaga cttttaaact ttaaacagac aggtctgaga
      781 gcctgaactc tcatttttcc tttgacttca gcctccaagg agttccacca actttggcgc
      841 gccggcttca ctttcattaa gtgaaagaga ggtgcccaga catgggtgac tggagtgcct
      901 tggggaagct tctggacaag gtccaagcct actccaccgc tggagggaag gtgtggctgt
      961 cagtgctctt catattcaga atcctgctcc tggggacagc tgttgagtca gcttggggtg
     1021 atgaacagtc tgcctttcgc tgtaacactc aacaacctgg ctgcgaaaac gtctgctatg
     1081 acaagtcctt ccccatctct cacgtgcgct tctgggtcct tcagatcata ttcgtgtctg
     1141 tgcccacact cctgtacttg gcccatgtgt tctatgtgat gaggaaggaa gagaagctaa
     1201 acaagaaaga agaggagctc aaagtggccc agactgacgg ggtcaacgtg gagatgcacc
     1261 tgaagcagat tgaaatcaag aagttcaagt acgggattga agagcacggc aaggtgaaaa
     1321 tgaggggcgg cttgctgaga acctacatca tcagcatcct cttcaagtct gtcttcgagg
     1381 tggccttcct gctcatccag tggtacatct atgggttcag cttgagcgcg gtctacacct
     1441 gcaagagaga tccctgcccc caccaggtag actgcttcct ctcacgtccc acggagaaaa
     1501 ccatcttcat catcttcatg ctggtggtgt ccttggtgtc tctcgctttg aacatcattg
     1561 agctcttcta cgtcttcttc aagggcgtta aggatcgcgt gaagggaaga agcgatcctt
     1621 accacgccac cactggccca ctgagcccat caaaagactg cggatctcca aaatacgcct
     1681 acttcaatgg ctgctcctca ccaacggctc cactctcgcc tatgtctcct cctgggtaca
     1741 agctggttac tggtgacaga aacaattcct cgtgccgcaa ttacaacaag caagctagcg
     1801 agcaaaactg ggcgaactac agcgcagagc aaaatcgcat ggggcaggcc ggaagcacca
     1861 tctccaactc gcacgcccag ccgttcgatt tccccgacga caaccagaat gccaaaaaag
     1921 ttgctgctgg acatgaactc cagccattag ccatcgtgga ccaacgacct tccagcagag
     1981 ccagcagccg cgccagcagc aggcctcggc ctgatgacct ggagattgcg gatccaccgg
     2041 tcgccaccat gagcaagggc gccgagctgt tcaccggcat cgtgcccatc ctgatcgagc
     2101 tgaatggcga tgtgaatggc cacaagttca gcgtgagcgg cgagggcgag ggcgatgcca
     2161 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgcct gtgccctggc
     2221 ccaccctggt ggccaccctg agctacggcg tgcagtgctt ctcacgctac cccgatcaca
     2281 tgaagcagca cgacttcttc aagagcgcca tgcctgaggg ctacatccag gagcgcacca
     2341 tcttcttcga ggatgacggc aactacaaga cgcgcgccga ggtgaagttc gagggcgata
     2401 ccctggtgag tcgcatcgag ctgaccggca ctgatttcaa ggaggatggc aacatcctgg
     2461 gcaataagat ggagtacaac tacaacgcca ccaatgtgta catcgtggcc gacaaggcca
     2521 ggaatggcat caaggtgaac ttcaaggtcc gccacaacat caaggatggc agcgtgcagc
     2581 tggccgacca ctaccagcag aataccccca tcggcgatgg ccctgtgctg ctgcccgata
     2641 accactacct gtccacccag agcgccctgt ccaaggaccc caacgagaag cgcgatcaca
     2701 tgatctacct cgagttcgtg accgccgccg ccatcaccca cggcatggat gagctgtaca
     2761 agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     2821 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2881 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2941 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3001 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3061 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3121 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3181 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3241 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3301 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3361 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3421 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     3481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3541 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3601 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3661 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3721 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3781 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3841 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3901 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3961 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     4021 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     4081 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     4141 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     4201 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     4261 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     4321 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4381 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4441 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4501 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     4561 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4621 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4681 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4741 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4801 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     4861 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     4921 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     4981 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     5041 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     5101 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     5161 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     5221 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     5281 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5341 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5401 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5461 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5521 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5581 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5641 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5701 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5761 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5821 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5881 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5941 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6001 ggccttttta cggttcctgg ccttttgctg gccttttgct ca
//
