LOCUS       sequence-210623-        6306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 400 / 490, Emission: 
            468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6306)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-C-Src-7
FEATURES             Location/Qualifiers
     source          1..6306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             693..734
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             2313..3029
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    3152..3273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3189..3208)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3243..3262
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3280..3735)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3417..3438)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3629..3648
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3762..3866
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3868..4225
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3868..4059
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3891..3911)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4076..4211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4260..5054
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4260..5054
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4314..4333)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4924..4943
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5242..5261)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5286..5333
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5662..6250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6151..6170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctcgagctca agcttcgaat tcatggggag cagcaagagc aagcccaagg
      721 accccagcca gcgccggcgc agcctggagc cacccgacag cacccaccac gggggattcc
      781 cagcctcgca gacccccaac aagacagcag cccccgacac gcaccgcacc cccagccgct
      841 cctttgggac cgtggccacc gagcccaagc tcttcggggg cttcaacact tctgacaccg
      901 tcacgtcgcc gcagcgtgcc ggggcactgg ctggcggcgt caccactttc gtggctctct
      961 atgactacga gtcccggact gaaacggact tgtccttcaa gaaaggagaa cgcctgcaga
     1021 ttgtcaacaa cacggaaggt gactggtggc tggctcattc cctcactaca ggacagacgg
     1081 gctacatccc cagtaactat gtcgcgccct cagactccat ccaggctgaa gagtggtact
     1141 ttgggaagat cactcgtcgg gagtccgagc ggctgctgct caaccccgaa aacccccggg
     1201 gaaccttctt ggtccgggag agcgagacga caaaaggtgc ctattgcctc tccgtttctg
     1261 actttgacaa cgccaagggg ctcaatgtga agcactacaa gatccgcaag ctggacagcg
     1321 gcggcttcta catcacctca cgcacacagt tcagcagcct gcagcagctg gtggcctact
     1381 actccaaaca tgctgatggc ttgtgccacc gcctgaccaa cgtctgcccc acgtccaagc
     1441 cccagaccca gggactcgcc aaggacgcgt gggaaatccc ccgggagtcg ctgcggctgg
     1501 aggtgaagct ggggcagggc tgctttggag aggtctggat ggggacctgg aacggcacca
     1561 ccagagtggc cataaagact ctgaagcccg gcaccatgtc cccggaggcc ttcctgcagg
     1621 aagcccaagt gatgaagaag ctccggcatg agaagctggt tcagctgtac gcagtggtgt
     1681 cggaagagcc catctacatc gtcactgagt acatgagcaa ggggagcctc ctggatttcc
     1741 tgaagggaga gatgggcaag tacctgcggc tgccacagct cgtcgatatg gctgctcaga
     1801 ttgcatccgg catggcctat gtggagagga tgaactacgt gcaccgagac ctgcgggcgg
     1861 ccaacatcct ggtgggggag aacctggtgt gcaaggtggc tgactttggg ctggcacgcc
     1921 tcatcgagga caacgagtac acagcacggc aaggtgccaa gttccccatc aagtggacag
     1981 cccccgaggc agccctctat ggccggttca ccatcaagtc ggatgtctgg tccttcggca
     2041 tcctgctgac tgagctgacc accaagggcc gggtgccata cccagggatg gtcaacaggg
     2101 aggtgctgga ccaggtggag aggggctacc gcatgccctg cccgcccgag tgccccgagt
     2161 cgctgcatga cctcatgtgc cagtgctggc ggaaggaccc tgaggagcgg cccacttttg
     2221 agtacctgca ggccttcctg gaggactact tcacctcgac agagccccag taccagcctg
     2281 gagagaacct acgggatcca ccggtcgcca ccatgagcaa gggcgccgag ctgttcaccg
     2341 gcatcgtgcc catcctgatc gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga
     2401 gcggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     2461 ccggcaagct gcctgtgccc tggcccaccc tggtggccac cctgagctac ggcgtgcagt
     2521 gcttctcacg ctaccccgat cacatgaagc agcacgactt cttcaagagc gccatgcctg
     2581 agggctacat ccaggagcgc accatcttct tcgaggatga cggcaactac aagacgcgcg
     2641 ccgaggtgaa gttcgagggc gataccctgg tgagtcgcat cgagctgacc ggcactgatt
     2701 tcaaggagga tggcaacatc ctgggcaata agatggagta caactacaac gccaccaatg
     2761 tgtacatcgt ggccgacaag gccaggaatg gcatcaaggt gaacttcaag gtccgccaca
     2821 acatcaagga tggcagcgtg cagctggccg accactacca gcagaatacc cccatcggcg
     2881 atggccctgt gctgctgccc gataaccact acctgtccac ccagagcgcc ctgtccaagg
     2941 accccaacga gaagcgcgat cacatgatct acctcgagtt cgtgaccgcc gccgccatca
     3001 cccacggcat ggatgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
     3061 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     3121 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     3181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3241 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     3301 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     3361 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     3421 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     3481 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     3541 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     3601 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     3661 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     3721 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3781 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     3841 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     3901 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3961 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4021 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4081 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4141 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4201 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     4261 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4321 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4381 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4441 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4501 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4561 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4621 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     4681 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     4741 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     4801 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     4861 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     4921 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     4981 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5041 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     5101 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5161 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5221 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     5281 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     5341 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     5401 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     5461 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     5521 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5581 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5641 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5701 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5761 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5821 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5881 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5941 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6001 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6061 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6301 tgctca
//
