LOCUS       sequence-210637-        7929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7929)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..7929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             671..1384
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    4776..4897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4813..4832)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4867..4886
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4904..5359)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5041..5062)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5253..5272
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5386..5490
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5492..5849
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5492..5683
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5515..5535)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5700..5835
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5883..6677
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5883..6677
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5937..5956)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6547..6566
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6865..6884)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6909..6956
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7285..7873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7774..7793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggtcgccacc atgagcaagg gcgccgagct gttcaccggc atcgtgccca tcctgatcga
      721 gctgaatggc gatgtgaatg gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc
      781 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg
      841 gcccaccctg gtggccaccc tgagctacgg cgtgcagtgc ttctcacgct accccgatca
      901 catgaagcag cacgacttct tcaagagcgc catgcctgag ggctacatcc aggagcgcac
      961 catcttcttc gaggatgacg gcaactacaa gacgcgcgcc gaggtgaagt tcgagggcga
     1021 taccctggtg agtcgcatcg agctgaccgg cactgatttc aaggaggatg gcaacatcct
     1081 gggcaataag atggagtaca actacaacgc caccaatgtg tacatcgtgg ccgacaaggc
     1141 caggaatggc atcaaggtga acttcaaggt ccgccacaac atcaaggatg gcagcgtgca
     1201 gctggccgac cactaccagc agaatacccc catcggcgat ggccctgtgc tgctgcccga
     1261 taaccactac ctgtccaccc agagcgccct gtccaaggac cccaacgaga agcgcgatca
     1321 catgatctac ctcgagttcg tgaccgccgc cgccatcacc cacggcatgg atgagctgta
     1381 caagtccgga ctcagatctc gagctcaagc ttcgaattcg ggaaactcga gaacaaagct
     1441 tgaaaacggc cgcatgccag tgtttcatac gcgcacgatc gagagcatcc tggagccggt
     1501 ggcacagcag atctcccacc tggtgataat gcacgaggag ggcgaggtgg acggcaaagc
     1561 cattcctgac ctcaccgcgc ccgtggccgc cgtgcaggcg gccgtcagca acctcgtccg
     1621 ggttggaaaa gagactgttc aaaccactga ggatcagatt ttgaagagag atatgccacc
     1681 agcatttatt aaggttgaga atgcttgcac caagcttgtc caggcagctc agatgcttca
     1741 gtcagaccct tactcagtgc ctgctcgaga ttatctaatt gatgggtcaa ggggcatcct
     1801 ctctggaaca tcagacctgc tccttacctt cgatgaggct gaggtccgta aaattattag
     1861 agtttgcaaa ggaattttgg aatatcttac agtggcagag gtggtggaga ctatggaaga
     1921 tttggtcact tacacaaaga atcttgggcc aggaatgact aagatggcca agatgattga
     1981 cgagagacag caggagctca ctcaccagga gcaccgagtg atgttggtga actcgatgaa
     2041 caccgtgaaa gagttgctgc cagttctcat ttcagctatg aagatttttg taacaactaa
     2101 aaactcaaaa aaccaaggca tagaggaagc tttaaaaaat cgcaatttta ctctagaaaa
     2161 aatgagtgct gaaattaatg agataattcg tgtgttacaa ctcacctctt gggatgaaga
     2221 tgcctgggcc agcaaggaca ctgaagccat gaagagagca ttggcctcca tagactccaa
     2281 actgaaccag gccaaaggtt ggctccgtga ccctagtgcc tccccagggg atgctggtga
     2341 gcaggccatc agacagatct tagatgaagc tggaaaagtt ggtgaactct gtgcaggcaa
     2401 agaacgcagg gagattctgg gaacttgcaa aatgctaggg cagatgactg atcaagtggc
     2461 tgacctccgt gccagaggac aaggatcctc accggtggcc atgcagaaag ctcagcaggt
     2521 atctcagggt ctggatgtgc tcacagcaaa agtggaaaat gcagctcgca agctggaagc
     2581 catgaccaac tcaaagcaga gcattgcaaa gaagatcgat gctgctcaga actggcttgc
     2641 agatccaaat ggtggaccgg aaggagaaga gcagattcga ggtgctttgg ctgaagctcg
     2701 gaaaatagca gaattatgtg atgatcctaa agaaagagat gacattctac gttcccttgg
     2761 ggaaatatct gctctgactt ctaaattagc agatctacga agacagggga aaggagattc
     2821 tccagaggct cgagccttgg ccaaacaggt ggccacggcc ctgcagaacc tgcagaccaa
     2881 aaccaaccgg gctgtggcca acagcagacc ggccaaagca gctgtacacc ttgagggcaa
     2941 gattgagcaa gcacagcggt ggattgataa tcccacagtg gatgaccgtg gagtcggtca
     3001 ggctgccatc cgggggcttg tggccgaagg gcatcgtctg gctaatgtta tgatggggcc
     3061 ttatcggcaa gatcttctcg ccaagtgtga ccgagtggac cagctgacag cccagctggc
     3121 tgacctggct gccagagggg aaggggagag tcctcaggca cgagcacttg catctcagct
     3181 ccaagactcc ttaaaggatc taaaagctcg gatgcaggag gccatgactc aggaagtgtc
     3241 agatgttttc agcgatacca caactcccat caagctgttg gcagtggcag ccacggcgcc
     3301 tcctgatgcg cctaacaggg aagaggtatt tgatgagagg gcagctaact ttgaaaacca
     3361 ttcaggaaag cttggtgcta cggccgagaa ggcggctgcg gttggtactg ctaataaatc
     3421 aacagtggaa ggcattcagg cctcagtgaa gacggcccga gaactcacac cccaggtggt
     3481 ctcggctgct cgtatcttac ttaggaaccc tggaaatcaa gctgcttatg aacattttga
     3541 gaccatgaag aaccagtgga tcgataatgt tgaaaaaatg acagggctgg tggacgaagc
     3601 cattgatacc aaatctctgt tggatgcttc agaagaagca attaaaaaag acctggacaa
     3661 gtgcaaggta gctatggcca acattcagcc tcagatgctg gttgctgggg caaccagtat
     3721 tgctcgtcgg gccaaccgga tcctgctggt ggctaagagg gaggtggaaa attccgagga
     3781 tcccaagttc cgtgaggctg tgaaagctgc ctctgatgaa ttgagcaaaa ccatctcccc
     3841 gatggtgatg gatgcaaaag ctgtggctgg aaacatttcc gaccctggac tgcaaaagag
     3901 cttcctggac tcaggatatc ggatcctggg agctgtggcc aaggtcagag aagccttcca
     3961 acctcaggag cctgacttcc cgccgcctcc accagacctt gaacaactcc gactaacaga
     4021 tgagcttgct cctcccaaac cacctctgcc tgaaggtgag gtccctccac ctaggcctcc
     4081 accaccagag gaaaaggatg aagagttccc tgagcagaag gccggggagg tgattaacca
     4141 gccaatgatg atggctgcca gacagctcca tgatgaagct cgcaaatggt ccagcaaggg
     4201 caatgacatc attgcagcag ccaagcgcat ggctctgctg atggctgaga tgtctcggct
     4261 ggtaagaggg ggcagtggta ccaagcgggc actcattcag tgtgccaagg acatcgccaa
     4321 ggcctcagat gaggtgactc ggttggccaa ggaggttgcc aagcagtgca cagataaacg
     4381 gattagaacc aacctcttac aggtatgtga gcgaatccca accataagca cccagctcaa
     4441 aatcctgtcc acagtgaagg ccaccatgct gggccggacc aacatcagtg atgaggagtc
     4501 tgagcaggcc acagagatgc tggttcacaa tgcccagaac ctcatgcagt ctgtgaagga
     4561 gactgtgcgg gaagctgaag ctgcttcaat caaaattcga acagatgctg gatttacact
     4621 gcgctgggtt agaaagactc cctggtacca gtaggcggcc gcgactctag atcataatca
     4681 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     4741 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     4801 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4861 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
     4921 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     4981 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     5041 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     5101 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     5161 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     5221 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     5281 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     5341 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5401 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5461 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     5521 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5581 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5641 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5701 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5761 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5821 gcttttttgg aggcctaggc ttttgcaaag atcatcaaga gacaggatga ggatcgtttc
     5881 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     5941 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6001 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6061 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6121 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     6181 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     6241 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     6301 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     6361 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     6421 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     6481 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     6541 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     6601 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     6661 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     6721 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     6781 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     6841 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     6901 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     6961 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     7021 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     7081 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     7141 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7201 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7261 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7321 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7381 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7441 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7501 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7561 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7621 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7681 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7741 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7801 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7861 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7921 ttttgctca
//
