LOCUS       sequence-210651-        5992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 400 / 490, Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5992)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Keratin-17
FEATURES             Location/Qualifiers
     source          1..5992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1995..2711
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    2834..2955
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2871..2890)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2925..2944
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2962..3417)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3099..3120)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3311..3330
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3444..3548
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3550..3907
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3550..3741
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3573..3593)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3758..3893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3942..4736
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3942..4736
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3996..4015)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4606..4625
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4924..4943)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4968..5015
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5344..5932
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5833..5852
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc accatgagct
      661 tcaccactcg ctccaccttc tccaccaact accggtccct gggctctgtc caggcgccca
      721 gctacggcgc ccggccggtc agcagcgcgg ccagcgtcta tgcaggcgct gggggctctg
      781 gttcccggat ctccgtgtcc cgctccacca gcttcagggg cggcatgggg tccgggggcc
      841 tggccaccgg gatagccggg ggtctggcag gaatgggagg catccagaac gagaaggaga
      901 ccatgcaaag cctgaacgac cgcctggcct cttacctgga cagagtgagg agcctggaga
      961 ccgagaaccg gaggctggag agcaaaatcc gggagcactt ggagaagaag ggaccccagg
     1021 tcagagactg gagccattac ttcaagatca tcgaggacct gagggctcag atcttcgcaa
     1081 atactgtgga caatgcccgc atcgttctgc agattgacaa tgcccgtctt gctgctgatg
     1141 actttagagt caagtatgag acagagctgg ccatgcgcca gtctgtggag aacgacatcc
     1201 atgggctccg caaggtcatt gatgacacca atatcacacg actgcagctg gagacagaga
     1261 tcgaggctct caaggaggag ctgctcttca tgaagaagaa ccacgaagag gaagtaaaag
     1321 gcctacaagc ccagattgcc agctctgggt tgaccgtgga ggtagatgcc cccaaatctc
     1381 aggacctcgc caagatcatg gcagacatcc gggcccaata tgacgagctg gctcggaaga
     1441 accgagagga gctagacaag tactggtctc agcagattga ggagagcacc acagtgctca
     1501 ccacacagtc tgctgaggtt ggagctgctg agacgacgct cacagagctg agacgtacag
     1561 tccagtcctt ggagatcgac ctggactcca tgagaaatct gaaggccagc ttggagaaca
     1621 gcctgaggga ggtggaggcc cgctacgccc tacagatgga gcagctcaac gggatcctgc
     1681 tgcaccttga gtcagagctg gcacagaccc gggcagaggg acagcgccag gcccaggagt
     1741 atgaggccct gctgaacatc aaggtcaagc tggaggctga gatcgccacc taccgccgcc
     1801 tgctggaaga tggcgaggac tttaatcttg gtgatgcctt ggacagcagc aactccatgc
     1861 aaaccatcca aaagaccacc acccgccgga tagtggatgg caaagtggtg tctgagacca
     1921 atgacaccaa agttctgagg catcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
     1981 caccggtcgc caccatgagc aagggcgccg agctgttcac cggcatcgtg cccatcctga
     2041 tcgagctgaa tggcgatgtg aatggccaca agttcagcgt gagcggcgag ggcgagggcg
     2101 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcctgtgc
     2161 cctggcccac cctggtggcc accctgagct acggcgtgca gtgcttctca cgctaccccg
     2221 atcacatgaa gcagcacgac ttcttcaaga gcgccatgcc tgagggctac atccaggagc
     2281 gcaccatctt cttcgaggat gacggcaact acaagacgcg cgccgaggtg aagttcgagg
     2341 gcgataccct ggtgagtcgc atcgagctga ccggcactga tttcaaggag gatggcaaca
     2401 tcctgggcaa taagatggag tacaactaca acgccaccaa tgtgtacatc gtggccgaca
     2461 aggccaggaa tggcatcaag gtgaacttca aggtccgcca caacatcaag gatggcagcg
     2521 tgcagctggc cgaccactac cagcagaata cccccatcgg cgatggccct gtgctgctgc
     2581 ccgataacca ctacctgtcc acccagagcg ccctgtccaa ggaccccaac gagaagcgcg
     2641 atcacatgat ctacctcgag ttcgtgaccg ccgccgccat cacccacggc atggatgagc
     2701 tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     2761 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     2821 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2881 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2941 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     3001 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     3061 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     3121 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3181 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3241 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     3301 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     3361 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     3421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3541 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     3601 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3661 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3721 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3781 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3841 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3901 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     3961 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4021 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     4081 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     4141 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4201 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4261 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4321 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4381 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     4441 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     4501 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     4561 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     4621 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4681 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     4741 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     4801 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     4861 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     4921 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     4981 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     5041 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     5101 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     5161 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     5221 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5281 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5341 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5401 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5461 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5521 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5581 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5641 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5701 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5761 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5821 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5881 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5941 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tg
//
