LOCUS       sequence-210654-        6387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6387)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..6387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      692..701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2422..2431
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2428..3111
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     polyA_signal    3233..3354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3270..3289)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3324..3343
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3361..3816)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3498..3519)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3710..3729
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3843..3947
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3949..4306
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3949..4140
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3972..3992)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4157..4292
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4341..5135
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4341..5135
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4395..4414)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5005..5024
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5323..5342)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5367..5414
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5743..6331
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6232..6251
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctcgagctca agcttcgaat tccatggcgg ccccccgccc gtctcccgcg
      721 atccccgttt cggtctcggc tccggctttt tacgccccgc agaagaagtt cggccctgtg
      781 gtggccccaa agcccaaagt gaatcccttc cggcccaggg acagcgagcc tcccccggca
      841 cccggggccc agcgcgcaca gatgggccgg gtgggcgata ttcccccgcc gcccccggaa
      901 gactttcccc tgcctccacc tccccttgct ggggatggcg acgatgcaga gggtgctctg
      961 ggaggtgcct tcccgccgcc ccctcccccg atcgaggaat catttccccc tgcgcctctg
     1021 gaggaggaga tcttcccttc cccgccgcct cctccggagg aggagggagg gcctgaggcc
     1081 cccataccgc ccccaccaca gcccagggag aaggtgagca gtattgattt ggagatcgac
     1141 tctctgtcct cactgctgga tgacatgacc aagaatgatc ctttcaaagc ccgggtgtca
     1201 tctggatatg tgcccccacc agtggccact ccattcagtt ccaagtccag taccaagcct
     1261 gcagccgggg gcacagcacc cctgcctcct tggaagtccc cttccagctc ccagcctctg
     1321 ccccaggttc cggctccggc tcagagccag acacagttcc atgttcagcc ccagccccag
     1381 cccaagcctc aggtccaact ccatgtccag tcccagaccc agcctgtgtc tttggctaac
     1441 acccagcccc gagggccccc agcctcatct ccggctccag cccctaagtt ttctccagtg
     1501 actcctaagt ttactcctgt ggcttccaag ttcagtcctg gagccccagg tggatctggg
     1561 tcacaaccaa atcaaaaatt ggggcacccc gaagctcttt ctgctggcac aggctcccct
     1621 caacctccca gcttcaccta tgcccagcag agggagaagc cccgagtgca ggagaagcag
     1681 caccccgtgc ccccaccggc tcagaaccaa aaccaggtgc gctcccctgg ggccccaggg
     1741 cccctgactc tgaaggaggt ggaggagctg gagcagctga cccagcagct aatgcaggac
     1801 atggagcatc ctcagaggca gaatgtggct gtcaacgaac tctgcggccg atgccatcaa
     1861 cccctggccc gggcgcagcc agccgtccgc gctctagggc agctgttcca catcgcctgc
     1921 ttcacctgcc accagtgtgc gcagcagctc cagggccagc agttctacag tctggagggg
     1981 gcgccgtact gcgagggctg ttacactgac accctggaga agtgtaacac ctgcggggag
     2041 cccatcactg accgcatgct gagggccacg ggcaaggcct atcacccgca ctgcttcacc
     2101 tgtgtggtct gcgcccgccc cctggagggc acctccttca tcgtggacca ggccaaccgg
     2161 ccccactgtg tccccgacta ccacaagcag tacgccccga ggtgctccgt ctgctctgag
     2221 cccatcatgc ctgagcctgg ccgagatgag actgtgcgag tggtcgccct ggacaagaac
     2281 ttccacatga agtgttacaa gtgtgaggac tgcgggaagc ccctgtcgat tgaggcagat
     2341 gacaatggct gcttccccct ggacggtcac gtgctctgtc ggaagtgcca cactgctaga
     2401 gcccagacgg atccaccggt cgccaccatg gtgagtgcga ttaagccaga catgaggatc
     2461 aaactccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
     2521 ggcaagcctt atgagggaaa acagaccatg gatcttgaag tcaaagaggg cggacctctg
     2581 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgttaaa
     2641 tatccagaca acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
     2701 cgaagcttga ctttcgaaga cgggggcatt tgctatgcca gaaacgacat aacaatggaa
     2761 ggggacactt tctataataa agttcgattt tatggtacca actttcccgc caatggtcca
     2821 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
     2881 ggagtgctga cgggtgatat tcatatggct ttgttgcttg aaggaaatgc ccattaccga
     2941 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac
     3001 tttgtggacc acgccattga gattttaagc catgacaaag attacaacaa ggttaagctg
     3061 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgata agcggccgcg
     3121 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3181 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3241 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3301 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt
     3361 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3421 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3481 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3541 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3601 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3661 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     3721 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     3781 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     3841 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3901 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     3961 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4021 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4081 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4141 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4201 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4261 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     4321 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4381 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4441 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4501 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     4561 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4621 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4681 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4741 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4801 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4861 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4921 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4981 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5041 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5101 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5161 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     5221 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5281 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     5341 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     5401 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     5461 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     5521 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     5581 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5641 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5701 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5761 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5821 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5881 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5941 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6001 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6061 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6121 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6181 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6241 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6301 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6361 cctggccttt tgctggcctt ttgctca
//
