LOCUS       sequence-210655-        7918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7918)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Vinculin-N-21
FEATURES             Location/Qualifiers
     source          1..7918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3925..4641
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    4764..4885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4801..4820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4855..4874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4892..5347)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5029..5050)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5241..5260
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5374..5478
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5480..5837
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5480..5671
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5503..5523)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5688..5823
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5872..6666
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5872..6666
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5926..5945)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6536..6555
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6854..6873)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6898..6945
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7274..7862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7763..7782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcaccgcc
      661 accatgccag tgtttcatac gcgcacgatc gagagcatcc tggagccggt ggcacagcag
      721 atctcccacc tggtgataat gcacgaggag ggcgaggtgg acggcaaagc cattcctgac
      781 ctcaccgcgc ccgtggccgc cgtgcaggcg gccgtcagca acctcgtccg ggttggaaaa
      841 gagactgttc aaaccactga ggatcagatt ttgaagagag atatgccacc agcatttatt
      901 aaggttgaga atgcttgcac caagcttgtc caggcagctc agatgcttca gtcagaccct
      961 tactcagtgc ctgctcgaga ttatctaatt gatgggtcaa ggggcatcct ctctggaaca
     1021 tcagacctgc tccttacctt cgatgaggct gaggtccgta aaattattag agtttgcaaa
     1081 ggaattttgg aatatcttac agtggcagag gtggtggaga ctatggaaga tttggtcact
     1141 tacacaaaga atcttgggcc aggaatgact aagatggcca agatgattga cgagagacag
     1201 caggagctca ctcaccagga gcaccgagtg atgttggtga actcgatgaa caccgtgaaa
     1261 gagttgctgc cagttctcat ttcagctatg aagatttttg taacaactaa aaactcaaaa
     1321 aaccaaggca tagaggaagc tttaaaaaat cgcaatttta ctctagaaaa aatgagtgct
     1381 gaaattaatg agataattcg tgtgttacaa ctcacctctt gggatgaaga tgcctgggcc
     1441 agcaaggaca ctgaagccat gaagagagca ttggcctcca tagactccaa actgaaccag
     1501 gccaaaggtt ggctccgtga ccctagtgcc tccccagggg atgctggtga gcaggccatc
     1561 agacagatct tagatgaagc tggaaaagtt ggtgaactct gtgcaggcaa agaacgcagg
     1621 gagattctgg gaacttgcaa aatgctaggg cagatgactg atcaagtggc tgacctccgt
     1681 gccagaggac aaggatcctc accggtggcc atgcagaaag ctcagcaggt atctcagggt
     1741 ctggatgtgc tcacagcaaa agtggaaaat gcagctcgca agctggaagc catgaccaac
     1801 tcaaagcaga gcattgcaaa gaagatcgat gctgctcaga actggcttgc agatccaaat
     1861 ggtggaccgg aaggagaaga gcagattcga ggtgctttgg ctgaagctcg gaaaatagca
     1921 gaattatgtg atgatcctaa agaaagagat gacattctac gttcccttgg ggaaatatct
     1981 gctctgactt ctaaattagc agatctacga agacagggga aaggagattc tccagaggct
     2041 cgagccttgg ccaaacaggt ggccacggcc ctgcagaacc tgcagaccaa aaccaaccgg
     2101 gctgtggcca acagcagacc ggccaaagca gctgtacacc ttgagggcaa gattgagcaa
     2161 gcacagcggt ggattgataa tcccacagtg gatgaccgtg gagtcggtca ggctgccatc
     2221 cgggggcttg tggccgaagg gcatcgtctg gctaatgtta tgatggggcc ttatcggcaa
     2281 gatcttctcg ccaagtgtga ccgagtggac cagctgacag cccagctggc tgacctggct
     2341 gccagagggg aaggggagag tcctcaggca cgagcacttg catctcagct ccaagactcc
     2401 ttaaaggatc taaaagctcg gatgcaggag gccatgactc aggaagtgtc agatgttttc
     2461 agcgatacca caactcccat caagctgttg gcagtggcag ccacggcgcc tcctgatgcg
     2521 cctaacaggg aagaggtatt tgatgagagg gcagctaact ttgaaaacca ttcaggaaag
     2581 cttggtgcta cggccgagaa ggcggctgcg gttggtactg ctaataaatc aacagtggaa
     2641 ggcattcagg cctcagtgaa gacggcccga gaactcacac cccaggtggt ctcggctgct
     2701 cgtatcttac ttaggaaccc tggaaatcaa gctgcttatg aacattttga gaccatgaag
     2761 aaccagtgga tcgataatgt tgaaaaaatg acagggctgg tggacgaagc cattgatacc
     2821 aaatctctgt tggatgcttc agaagaagca attaaaaaag acctggacaa gtgcaaggta
     2881 gctatggcca acattcagcc tcagatgctg gttgctgggg caaccagtat tgctcgtcgg
     2941 gccaaccgga tcctgctggt ggctaagagg gaggtggaaa attccgagga tcccaagttc
     3001 cgtgaggctg tgaaagctgc ctctgatgaa ttgagcaaaa ccatctcccc gatggtgatg
     3061 gatgcaaaag ctgtggctgg aaacatttcc gaccctggac tgcaaaagag cttcctggac
     3121 tcaggatatc ggatcctggg agctgtggcc aaggtcagag aagccttcca acctcaggag
     3181 cctgacttcc cgccgcctcc accagacctt gaacaactcc gactaacaga tgagcttgct
     3241 cctcccaaac cacctctgcc tgaaggtgag gtccctccac ctaggcctcc accaccagag
     3301 gaaaaggatg aagagttccc tgagcagaag gccggggagg tgattaacca gccaatgatg
     3361 atggctgcca gacagctcca tgatgaagct cgcaaatggt ccagcaaggg caatgacatc
     3421 attgcagcag ccaagcgcat ggctctgctg atggctgaga tgtctcggct ggtaagaggg
     3481 ggcagtggta ccaagcgggc actcattcag tgtgccaagg acatcgccaa ggcctcagat
     3541 gaggtgactc ggttggccaa ggaggttgcc aagcagtgca cagataaacg gattagaacc
     3601 aacctcttac aggtatgtga gcgaatccca accataagca cccagctcaa aatcctgtcc
     3661 acagtgaagg ccaccatgct gggccggacc aacatcagtg atgaggagtc tgagcaggcc
     3721 acagagatgc tggttcacaa tgcccagaac ctcatgcagt ctgtgaagga gactgtgcgg
     3781 gaagctgaag ctgcttcaat caaaattcga acagatgctg gatttacact gcgctgggtt
     3841 agaaagactc cctggtacca gtccggtggc agcggaattc tgcagtcgac ggtaccgcgg
     3901 gcccgggatc caccggtcgc caccatgagc aagggcgccg agctgttcac cggcatcgtg
     3961 cccatcctga tcgagctgaa tggcgatgtg aatggccaca agttcagcgt gagcggcgag
     4021 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4081 ctgcctgtgc cctggcccac cctggtggcc accctgagct acggcgtgca gtgcttctca
     4141 cgctaccccg atcacatgaa gcagcacgac ttcttcaaga gcgccatgcc tgagggctac
     4201 atccaggagc gcaccatctt cttcgaggat gacggcaact acaagacgcg cgccgaggtg
     4261 aagttcgagg gcgataccct ggtgagtcgc atcgagctga ccggcactga tttcaaggag
     4321 gatggcaaca tcctgggcaa taagatggag tacaactaca acgccaccaa tgtgtacatc
     4381 gtggccgaca aggccaggaa tggcatcaag gtgaacttca aggtccgcca caacatcaag
     4441 gatggcagcg tgcagctggc cgaccactac cagcagaata cccccatcgg cgatggccct
     4501 gtgctgctgc ccgataacca ctacctgtcc acccagagcg ccctgtccaa ggaccccaac
     4561 gagaagcgcg atcacatgat ctacctcgag ttcgtgaccg ccgccgccat cacccacggc
     4621 atggatgagc tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat
     4681 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     4741 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     4801 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4861 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
     4921 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     4981 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     5041 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     5101 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     5161 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     5221 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     5281 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     5341 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5401 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5461 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     5521 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5581 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5641 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     5701 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5761 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5821 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     5881 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5941 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     6001 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     6061 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     6121 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6181 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6241 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6301 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6361 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     6421 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6481 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6541 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6601 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6661 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6721 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6781 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     6841 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     6901 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     6961 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     7021 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     7081 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     7141 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7201 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7261 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7321 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7381 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7441 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7501 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7561 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7621 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7681 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7741 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7801 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7861 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctca
//
