LOCUS       sequence-210665-       10783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago 
            maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 
            promoter, contains a short version of U6 promoter, is 
            self-replicating.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Schuster M, Schweizer G, Kahmann R
  TITLE     Comparative analyses of secreted proteins in plant pathogenic smut 
            fungi and related basidiomycetes.
  JOURNAL   Fungal Genet Biol. 2018 Mar;112:21-30. doi: 
            10.1016/j.fgb.2016.12.003. Epub 2017 Jan 6.
  PUBMED    28089076
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-03"; volume: "112:21-30. doi"; pages: " 
            10.1016/j.fgb.2016.12.003. Epub 2017 Jan 6"
COMMENT     Sequence Label: pMS73
FEATURES             Location/Qualifiers
     source          1..10783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     57..74
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    191..212
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(227..232,233..250,251..257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    265..281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     270..292
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     289..305
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     289..305
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1713..1733
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1737..5837
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1737..5837
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5847..5873
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5883..5930
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5951..6203
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6233..6250)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6233..6249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6242..6264)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6458..6477)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6577..6599
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6637..6655)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(9067..9135,9136..9927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9285..9304)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10098..10686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10587..10606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
       61 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      121 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      181 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      241 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      301 atgaccatga ttacgccaag cttcgaccag agagagaggc agagagagag agagagatgg
      361 ctcttcagcg ccgcttcgcg cgtccttcac gattattcgt gattactgtc caccggtccc
      421 tcttactcag aactgccgga acgaattcgt gatttacacc aaacacgcgc tgtcacagtc
      481 acgagtccat gagccgtgag ccccgcttca gatcctgttt tctcttattc agcgtaacaa
      541 cacaaaacag aatttcttct aaacaccctt gcaattcgcg cacacccctg tagcagtctg
      601 tcagcattca aaattccatt ctacaacggt acctgccaag agtcgaacgc cgagaaggcg
      661 aaatgcaccg gacactgggg atgctgctgg aagatgggcc gacaaaggac tcagctgcta
      721 ctgagtcata aatagttcgg attgacggct gagtcgaaag cgttcgtata tattggtctg
      781 attcacgatt cggaagaaat tctacaaggt tgcgcagctc aggtcatctt aagtttactc
      841 tcaccgtctc gagtggcggc agatgtgagt cgtgtgctac aaaacgtgaa tcgacacgcg
      901 cagggcggaa ccaaaaaaaa cccccacccc gctgtcaagt tgacaaatca acacatttgt
      961 tccaagttca aatcgggaaa tcaaaaatta gggccagatc agcgatcagg aatggtaatc
     1021 gggtaacaga ggtcgcaaaa tcgtctagaa aatggaagaa gaacgtggta actaccagcg
     1081 agttctgcaa acttcaaaaa aaaaatctgg gcacgatgaa agttgagcta acgctgacgc
     1141 tcacaaatgg cgtggctaaa ggaagcgaga caatcggaaa attgttctct cgggcaccac
     1201 aaagctgttg ttagtcgctg aagaacaatt ccaactgatt ccgccgcctt cctattgcgt
     1261 cagccttgta cctaagctgc cgagtaacgt cactcaacct ctcttttcag actgctttgc
     1321 tccgcgaata cttttcttct atgcgctcaa gaaaatgaca cagcacacca agctctgcaa
     1381 actttcttcg ctaatctgac gcgaaatgtg agccatttct tctcgcctgc aatggcaatg
     1441 cgtctgtgcg gcgatgagaa tcacgatgcg gaatgggtgg ctggaagttc atagagatgc
     1501 tgagttgttg gagcgacatg gtacataagc atgagtctgt cctgatttcc accctcccgt
     1561 ctttcatcaa ctttctcgtc tgacccttcc gttgcacata cccctttcgc aaccaagtta
     1621 cgacatcagc acacagcgat cctgctattc cacacaggac ttccttcccc ttcaccacag
     1681 caaaagtttt agatttcaca ggatccatgc cgcctaagaa gaaacgcaag gttgaggata
     1741 agaagtacag catcggactc gacatcggta ctaactcggt aggatgggca gtcatcacgg
     1801 atgaatacaa ggttccttcc aagaagttta aggtccttgg taacaccgac cgccattcta
     1861 tcaagaagaa cctcattggc gctttgctct ttgactcagg agaaaccgct gaggcgacac
     1921 gcctcaaacg cacggcacgt cgacgttata cacgcagaaa gaatcgtatc tgctatctgc
     1981 aggaaatctt ttcgaacgaa atggcaaaag ttgatgacag cttcttccat cgcctggagg
     2041 aatcgtttct cgtggaggag gacaagaagc acgagagaca tcctatcttc ggcaacattg
     2101 tcgatgaggt cgcttaccac gagaagtacc ctactatcta ccaccttaga aagaagctcg
     2161 tagactcaac tgacaaagcg gatcttcgtc tgatctattt ggctcttgcc cacatgatca
     2221 agttccgtgg tcattttctc atcgaaggcg accttaatcc cgacaactcg gacgtcgata
     2281 agctgtttat ccagctcgta cagacataca accagctctt cgaagaaaac ccaattaatg
     2341 cttccggtgt tgatgcaaaa gctatcctca gcgcgagatt gagcaagagc agacgcctcg
     2401 aaaacctcat cgcccaattg cctggtgaaa agaagaacgg cttgttcggc aatcttattg
     2461 ccctgagcct tggcctcact ccgaacttca agtcgaactt cgatcttgcg gaagatgcca
     2521 agctgcaact ctccaaggac acgtacgatg atgacttgga caatctcctt gcccagattg
     2581 gcgatcaata cgccgatctt ttcctcgcgg cgaagaactt gtcggacgca atcttgctct
     2641 cagacatcct tcgcgtcaac actgagatca ccaaagcccc tctctctgcc tcgatgatca
     2701 agcgctatga cgaacaccac caggatctca cgctccttaa ggcattggtg cgtcagcagt
     2761 tgcctgagaa gtacaaagag attttctttg atcagtcgaa gaacggatac gctggctaca
     2821 tcgacggtgg cgcttctcag gaggagttct acaagtttat caaacccatt cttgagaaga
     2881 tggatggcac ggaggagctc ctcgtcaagc tgaatcgcga ggacctcctc cgtaagcaac
     2941 gtacgttcga caatggctcg attccacacc agattcatct gggcgaactc cacgccatcc
     3001 tcaggaggca ggaggacttc tatcccttcc tcaaggataa tcgagagaaa attgagaaga
     3061 tcctcacatt ccgcatcccc tattatgtag gcccactcgc tcgcggaaac tctcgctttg
     3121 cctggatgac ccgcaagtcg gaagaaacaa tcaccccgtg gaacttcgaa gaggtggtgg
     3181 acaagggtgc atctgcgcag tcgtttattg agaggatgac aaactttgat aagaacctcc
     3241 cgaatgagaa agtcctgcca aaacattccc tcctgtatga atacttcacg gtctataacg
     3301 aactgacaaa ggtgaagtac gtgaccgagg gtatgcgtaa gcctgccttt ctttcgggtg
     3361 agcagaagaa agctattgtc gacttgttgt tcaagaccaa ccgcaaggtc actgtcaagc
     3421 aactgaagga agattacttc aagaaaatcg agtgttttga ttcggtagag atctcgggcg
     3481 tcgaggacag gttcaacgcc tctctcggca cctatcacga tcttctcaag atcatcaagg
     3541 acaaagactt tcttgacaac gaagagaacg aggatattct cgaggacatc gtgctcaccc
     3601 tcactttgtt cgaagatcgc gaaatgattg aggaacgtct taagacatat gctcacttgt
     3661 tcgacgacaa agtgatgaag cagctgaagc gtaggcgata cacaggttgg ggccgcctct
     3721 cgcgcaagct gattaacggt atccgcgaca agcaatccgg caagacaatc ttggatttcc
     3781 ttaagagcga cggttttgct aaccgcaact tcatgcagct catccacgac gacagcctta
     3841 cgttcaagga ggacatccag aaggcccagg tttccggaca aggtgactct ctccatgagc
     3901 acatcgctaa cctggcggga agccccgcga tcaagaaagg tatcctccag accgtcaaag
     3961 ttgtggacga gctggtcaag gtaatgggcc gacacaaacc ggagaacatt gttatcgaga
     4021 tggcacgaga gaatcagacg acccagaaag gccaaaagaa ctccagagaa cgtatgaaac
     4081 gaatcgaaga gggtatcaag gaactgggat cgcagatcct gaaggagcac cccgttgaga
     4141 acacgcagct ccaaaacgaa aagctgtacc tctactactt gcaaaatggt agggatatgt
     4201 acgtcgacca ggaactggat attaatcgtc tgtccgacta cgacgttgac cacatcgtgc
     4261 cccaatcgtt tctcaaggat gactcgatcg ataataaagt acttacgcgc tcagacaaga
     4321 accgaggtaa atcggacaat gtcccatcgg aggaagtcgt gaagaagatg aagaactatt
     4381 ggcgccaact tcttaacgca aagctgatca cccagaggaa attcgacaac ctcaccaaag
     4441 cagaacgcgg cggcctctcc gagctcgaca aggctggatt tatcaagcgt cagctcgtcg
     4501 aaacgcgtca gattaccaag cacgtcgcac agatcctgga tagccgcatg aacacaaagt
     4561 acgacgaaaa cgacaagctc atccgtgagg ttaaggtcat caccttgaag tcgaaactcg
     4621 tgtcggactt ccgcaaagat tttcagttct ataaagttag agagatcaac aactaccacc
     4681 atgcgcatga cgcctacctc aatgccgtcg tgggcaccgc acttattaag aaatacccga
     4741 agctcgagtc cgagtttgtc tacggcgatt acaaggtata cgacgttcgc aagatgattg
     4801 ccaaatcgga gcaggagatc ggtaaggcca ctgccaagta cttcttttac tcgaacatca
     4861 tgaatttctt caaaacagaa atcaccctcg ccaacggcga gattcgcaaa cgaccactca
     4921 tcgagactaa cggtgaaacg ggagagatcg tctgggataa gggccgagac tttgctacgg
     4981 ttcgaaaggt cctttcgatg cctcaagtga acatcgtcaa gaaaacggag gtccaaaccg
     5041 gtggcttcag caaggagtcg attctgccga aacgcaattc ggacaaattg attgcacgca
     5101 agaaggattg ggaccctaag aaatatggcg gcttcgattc accgacagtg gcctattcgg
     5161 ttctggtcgt cgcgaaagtg gagaagggca agtcaaagaa gctcaagtca gtgaaggagc
     5221 tcctgggaat caccatcatg gaacgttcct cttttgagaa gaaccctatc gactttctcg
     5281 aggctaaggg ctacaaagag gtcaagaaag atctcatcat caaactccca aaatactcac
     5341 ttttcgagct cgagaacggc cgtaaacgaa tgctggcgag cgcaggagag cttcaaaagg
     5401 gaaatgaact ggctttgccc tccaagtacg tcaacttcct ctacctcgca agccattatg
     5461 agaagttgaa gggtagcccc gaggacaacg aacaaaagca gctcttcgtg gagcaacaca
     5521 aacattacct ggatgaaatc atcgagcaaa tctcggagtt tagcaagcga gtgatcttgg
     5581 ctgatgccaa cctcgacaag gtgttgtctg cctacaacaa gcatcgagat aagccgattc
     5641 gcgagcaggc cgagaacatc atccacctct tcactctcac taacttgggt gcgcctgcgg
     5701 cgtttaaata ctttgacacg accatcgacc gcaagcgtta cacaagcacg aaggaagtcc
     5761 tcgacgctac actgatccat cagtcgatca ccggtctgta cgaaacccgc atcgatctgt
     5821 ctcaactggg cggtgacagc ggcggctacc catacgatgt gcccgattac gctagcggcg
     5881 gaaagcgtcc cgcagccact aagaaggctg gacaggccaa gaagaagaag tgagcggccg
     5941 cccggctgca gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     6001 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     6061 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     6121 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     6181 ggtgtcatct atgttactag atccgatgat aagctgtcaa acatgagaat tcactggccg
     6241 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6301 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6361 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     6421 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     6481 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6541 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     6601 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     6661 aggttaatgt catgataata atggtttctt aggcggccgc acgctaagtg gagttgtccg
     6721 agtcccgaat cacaagaatt aggctcgtgc tctgtgagat ctctcgcgaa acccagatga
     6781 aggaaaaaaa tcggaagatc gcggaagaag tggggttcgc atggtctaac attgtcgcat
     6841 tcctcacagt ttcggctgga aacggcaggg acaatcacga gaaatgcgcg acgagtattc
     6901 agttgtccaa aattatgtca gcttgaaagt tggaaacggc ggaagaaaat tcgtcaggag
     6961 ggcgttgcgg ctagggtgaa gaacggagac aatttcggct ttgaaattgg ctcgactctg
     7021 attggatcat gacgcactgc tggtccacaa ccgtgtcgaa ggtgccgtct ttactacagg
     7081 tccgctggaa gcaaaatgga aaaagccgct ggagcccgac tacagagccg ccgtgttttg
     7141 gtaatcagtc ggcaaatagg tcagcacagc gcagcgtgac aggttcttgc aatttacagc
     7201 acagctcgtc cgtctacgac tttgcacacc acaaagtgtg cggggagcaa aggagccgat
     7261 cttggtcgcg cgcaaagcca aggagtcttg aacctgagag tgtgcgtgtc ttgtgacgct
     7321 tgcccttctg tactttgctg tgacactacc accacatctg tcttggcttt ttgttcatac
     7381 atccacaccg accatgtcgc tattcaacgt cagcaacggt cttcgtaccg ctctccgacc
     7441 ttctgttgcc agctcttcgc gcgttgctgc cttttccaca accgccgctg cccgtctcgc
     7501 cacacccacc tctgacaacg ttggcagttc gggcaagcct cagcacttga agcagttcaa
     7561 gatctaccga tggaaccctg acaagccctc ggagaagcct cgtctgcagt cgtacacact
     7621 ggacctcaac cagaccggtc caatggttct cgacgcgctc atcaaaatca agaacgaaat
     7681 tgaccctacg ctcaccttcc gtcgctcgtg ccgtgagggt atctgcggtt cgtgcgctat
     7741 gaatattgac ggtgtcaaca ccctcgcctg cctctgccgg atcgacaagc agaatgacac
     7801 caagatctac cccttgccgc acatgtacat tgtcaaggac ctcgtgccag acttgaccca
     7861 gttctacaag cagtaccgat ccatcgagcc tttcctcaag tccaacaaca ccccttctga
     7921 gggtgaacat cttcagtcgc ccgaggagcg tcgtcgactc gacggtctgt acgagtgcat
     7981 tctgtgcgcg tgctgctcca catcctgccc ctcttactgg tggaatcagg acgagtacct
     8041 tggccccgcc gtgctcatgc aggcgtaccg atggatggcc gactcgcgtg acgactttgg
     8101 tgaggagcga agacagaagc tcgagaacac cttttcgctc taccgatgcc ttaccatcat
     8161 gaactgctcc aggacctgcc ccaagaacct caaccctggt aaggcaattg cacagatcaa
     8221 gaaggacatg gccgtcggcg cacccaaggc ttccgagcgc cctatcatgg cttcgtcgta
     8281 atcttgatat atcatatcgt tctttcctca gcacttcttt tgtcaatttc aaaagtatct
     8341 aattgcattc aactccgctt gtggtttgtt gttcagtgag agtggaaacg ctacgggcaa
     8401 gatgagggca gtgttctggc gacggaaaag tgtgcaagtg tctggcctgc gtcctcgctg
     8461 gttccagcag ccgatgcagg acgtgtacct agcgatttct tcgacagcct attgtggcag
     8521 ccgcgattcg ccacaatcgt acgtgcggcc gccaggtggc acttttcggg gaaatgtgcg
     8581 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8641 ataaccctga taaatgcttc aataatatta ccgatcctcg atctttgtgc aagctagccc
     8701 gcctcggcag caacaaagca gccgagcaag aagcagtact tgccttctga atcgtgaatg
     8761 ggttacgttc ttcaccgctg tgatcagcga atcatgaatc aaatcatgag ggcattgctg
     8821 atcatgaatc aaatcatgag ggcatttaaa aattcagtct gagtcgtgag tagcaagtcg
     8881 gttctggatc ggatggcatt catgaatcac agggtcgtga atcatgaatg ttcaagtccc
     8941 cttttctcga gaggctggtg gcatcggtgc gaatcacgaa tcatgattgt aattcattga
     9001 gtgaaggagt ttcgcagcca cccacagtac tagaatcacg aatgacaata ttgaaaaagg
     9061 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9121 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9181 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9241 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9301 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9361 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9421 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9481 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9541 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9601 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9661 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9721 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     9781 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     9841 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     9901 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     9961 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10021 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10081 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10141 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10201 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10261 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10321 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10381 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10441 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10501 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10561 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10621 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10681 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    10741 cacatgttct ttcctgcgtt atcccctgat tctgtggata acc
//
