LOCUS       sequence-210677-        9680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized AsCpf1 and As crRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, 
            Fodor E, Welker E
  TITLE     Cpf1 nucleases demonstrate robust activity to induce DNA 
            modification by exploiting homology directed repair pathways in 
            mammalian cells.
  JOURNAL   Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0.
  PUBMED    27630115
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Direct."; date: "2016-09-14"; volume: "11:46. doi"; pages: " 
            10.1186/s13062-016-0147-0"
COMMENT     Sequence Label: pTE4396
FEATURES             Location/Qualifiers
     source          1..9680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(213..461)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(271..290)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(441..461)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        524..827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        828..1031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     982..1002
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1076..1095
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1076..1094
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1142..5062
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4782..4791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5063..5110
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5117..5197
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5271..5288)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5277..5501
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5547..5975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5634..5653)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5844..5865
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5984..6004)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5989..6318
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6169..6304
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6231..6250
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6385..7179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6439..6458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7049..7068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7353..7474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7390..7409)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7444..7463
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7523..7539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7523..7539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7536..7558)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7547..7563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7571..7577,7578..7595,7596..7601))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7616..7637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7754..7771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7925..8513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8005..8024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8684..9475,9476..9544))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9307..9326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9545..9649)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattaaaaa aggagacggg
      181 atcccgtctc catctacaag agtagaaatt acggtgtttc gtcctttcca caagatatat
      241 aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa
      301 cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt
      361 actaatatct ttgtgtttac agtcaaatta attccaatta tctctctaac agccttgtat
      421 cgtatatgca aatatgaagg aatcatggga aataggccct cactagttat taatagtaat
      481 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      541 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      601 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      661 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      721 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      781 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      841 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      901 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      961 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1021 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
     1081 cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgccac
     1141 catgacacag ttcgagggct ttaccaacct gtatcaggtg agcaagacac tgcggtttga
     1201 gctgatccca cagggcaaga ccctgaagca catccaggag cagggcttca tcgaggagga
     1261 caaggcccgc aatgatcact acaaggagct gaagcccatc atcgatcgga tctacaagac
     1321 ctatgccgac cagtgcctgc agctggtgca gctggattgg gagaacctga gcgccgccat
     1381 cgactcctat agaaaggaga aaaccgagga gacaaggaac gccctgatcg aggagcaggc
     1441 cacatatcgc aatgccatcc acgactactt catcggccgg acagacaacc tgaccgatgc
     1501 catcaataag agacacgccg agatctacaa gggcctgttc aaggccgagc tgtttaatgg
     1561 caaggtgctg aagcagctgg gcaccgtgac cacaaccgag cacgagaacg ccctgctgcg
     1621 gagcttcgac aagtttacaa cctacttctc cggcttttat gagaacagga agaacgtgtt
     1681 cagcgccgag gatatcagca cagccatccc acaccgcatc gtgcaggaca acttccccaa
     1741 gtttaaggag aattgtcaca tcttcacacg cctgatcacc gccgtgccca gcctgcggga
     1801 gcactttgag aacgtgaaga aggccatcgg catcttcgtg agcacctcca tcgaggaggt
     1861 gttttccttc cctttttata accagctgct gacacagacc cagatcgacc tgtataacca
     1921 gctgctggga ggaatctctc gggaggcagg caccgagaag atcaagggcc tgaacgaggt
     1981 gctgaatctg gccatccaga agaatgatga gacagcccac atcatcgcct ccctgccaca
     2041 cagattcatc cccctgttta agcagatcct gtccgatagg aacaccctgt ctttcatcct
     2101 ggaggagttt aagagcgacg aggaagtgat ccagtccttc tgcaagtaca agacactgct
     2161 gagaaacgag aacgtgctgg agacagccga ggccctgttt aacgagctga acagcatcga
     2221 cctgacacac atcttcatca gccacaagaa gctggagaca atcagcagcg ccctgtgcga
     2281 ccactgggat acactgagga atgccctgta tgagcggaga atctccgagc tgacaggcaa
     2341 gatcaccaag tctgccaagg agaaggtgca gcgcagcctg aagcacgagg atatcaacct
     2401 gcaggagatc atctctgccg caggcaagga gctgagcgag gccttcaagc agaaaaccag
     2461 cgagatcctg tcccacgcac acgccgccct ggatcagcca ctgcctacaa ccctgaagaa
     2521 gcaggaggag aaggagatcc tgaagtctca gctggacagc ctgctgggcc tgtaccacct
     2581 gctggactgg tttgccgtgg atgagtccaa cgaggtggac cccgagttct ctgcccggct
     2641 gaccggcatc aagctggaga tggagccttc tctgagcttc tacaacaagg ccagaaatta
     2701 tgccaccaag aagccctact ccgtggagaa gttcaagctg aactttcaga tgcctacact
     2761 ggcctctggc tgggacgtga ataaggagaa gaacaatggc gccatcctgt ttgtgaagaa
     2821 cggcctgtac tatctgggca tcatgccaaa gcagaagggc aggtataagg ccctgagctt
     2881 cgagcccaca gagaaaacca gcgagggctt tgataagatg tactatgact acttccctga
     2941 tgccgccaag atgatcccaa agtgcagcac ccagctgaag gccgtgacag cccactttca
     3001 gacccacaca acccccatcc tgctgtccaa caatttcatc gagcctctgg agatcacaaa
     3061 ggagatctac gacctgaaca atcctgagaa ggagccaaag aagtttcaga cagcctacgc
     3121 caagaaaacc ggcgaccaga agggctacag agaggccctg tgcaagtgga tcgacttcac
     3181 aagggatttt ctgtccaagt ataccaagac aacctctatc gatctgtcta gcctgcggcc
     3241 atcctctcag tataaggacc tgggcgagta ctatgccgag ctgaatcccc tgctgtacca
     3301 catcagcttc cagagaatcg ccgagaagga gatcatggat gccgtggaga caggcaagct
     3361 gtacctgttc cagatctata acaaggactt tgccaagggc caccacggca agcctaatct
     3421 gcacacactg tattggaccg gcctgttttc tccagagaac ctggccaaga caagcatcaa
     3481 gctgaatggc caggccgagc tgttctaccg ccctaagtcc aggatgaaga ggatggcaca
     3541 ccggctggga gagaagatgc tgaacaagaa gctgaaggat cagaaaaccc caatccccga
     3601 caccctgtac caggagctgt acgactatgt gaatcacaga ctgtcccacg acctgtctga
     3661 tgaggccagg gccctgctgc ccaacgtgat caccaaggag gtgtctcacg agatcatcaa
     3721 ggataggcgc tttaccagcg acaagttctt tttccacgtg cctatcacac tgaactatca
     3781 ggccgccaat tccccatcta agttcaacca gagggtgaat gcctacctga aggagcaccc
     3841 cgagacacct atcatcggca tcgatcgggg cgagagaaac ctgatctata tcacagtgat
     3901 cgactccacc ggcaagatcc tggagcagcg gagcctgaac accatccagc agtttgatta
     3961 ccagaagaag ctggacaaca gggagaagga gagggtggca gcaaggcagg cctggtctgt
     4021 ggtgggcaca atcaaggatc tgaagcaggg ctatctgagc caggtcatcc acgagatcgt
     4081 ggacctgatg atccactacc aggccgtggt ggtgctggag aacctgaatt tcggctttaa
     4141 gagcaagagg accggcatcg ccgagaaggc cgtgtaccag cagttcgaga agatgctgat
     4201 cgataagctg aattgcctgg tgctgaagga ctatccagca gagaaagtgg gaggcgtgct
     4261 gaacccatac cagctgacag accagttcac ctcctttgcc aagatgggca cccagtctgg
     4321 cttcctgttt tacgtgcctg ccccatatac atctaagatc gatcccctga ccggcttcgt
     4381 ggaccccttc gtgtggaaaa ccatcaagaa tcacgagagc cgcaagcact tcctggaggg
     4441 cttcgacttt ctgcactacg acgtgaaaac cggcgacttc atcctgcact ttaagatgaa
     4501 cagaaatctg tccttccaga ggggcctgcc cggctttatg cctgcatggg atatcgtgtt
     4561 cgagaagaac gagacacagt ttgacgccaa gggcacccct ttcatcgccg gcaagagaat
     4621 cgtgccagtg atcgagaatc acagattcac cggcagatac cgggacctgt atcctgccaa
     4681 cgagctgatc gccctgctgg aggagaaggg catcgtgttc agggatggct ccaacatcct
     4741 gccaaagctg ctggagaatg acgattctca cgccatcgac accatggtgg ccctgatccg
     4801 cagcgtgctg cagatgcgga actccaatgc cgccacaggc gaggactata tcaacagccc
     4861 cgtgcgcgat ctgaatggcg tgtgcttcga ctcccggttt cagaacccag agtggcccat
     4921 ggacgccgat gccaatggcg cctaccacat cgccctgaag ggccagctgc tgctgaatca
     4981 cctgaaggag agcaaggatc tgaagctgca gaacggcatc tccaatcagg actggctggc
     5041 ctacatccag gagctgcgca acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
     5101 aaagaaaaag ggatcctacc catacgatgt tccagattac gcttatccct acgacgtgcc
     5161 tgattatgca tacccatatg atgtccccga ctatgcctaa gaattctgca gatatccagc
     5221 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     5281 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5341 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5401 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5461 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5521 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5581 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5641 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5701 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5761 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5821 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5881 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5941 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     6001 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6061 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6121 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6181 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6241 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6301 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     6361 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6421 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6481 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6541 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6601 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6661 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6721 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6781 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6841 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6901 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6961 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     7021 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     7081 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     7141 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     7201 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     7261 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     7321 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     7381 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7441 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     7501 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7561 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7621 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7681 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7741 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7801 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7861 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7921 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7981 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8041 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8101 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8161 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8221 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8281 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8341 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8401 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8461 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8521 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8581 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8641 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8701 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8761 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8821 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8881 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8941 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9001 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9061 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9121 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9181 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9241 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9301 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9361 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9421 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9481 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9541 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9601 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9661 gaaaagtgcc acctgacgtc
//
