LOCUS       sequence-210678-       11345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of myc-YWAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UbcP-Myc-YWAA-pGK-HYG
FEATURES             Location/Qualifiers
     source          1..11345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    58..175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        267..1478
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1315..1334
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1524..1533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1533..1562
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1584..1608
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2935..2959)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3103..3602
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3138..3161)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3535..3554
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3620..4645
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3620..4645
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4676..4709)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4765..5353
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4818..4838)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5236..5247)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5356..5372)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5357..5373)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5562..5795
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5867..5988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5904..5923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5958..5977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6028..6163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6183..6202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6184..6202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6225..6583)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6265..6286)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6477..6496
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6610..6714
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6716..7073
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6716..6907
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6739..6759)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6924..7059
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7108..7902
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7108..7902
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7162..7181)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7772..7791
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8090..8109)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8134..8181
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8510..9098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8999..9018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9273..9576
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9578..9776
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9731..9751
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9794..9974
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10021..10146
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10643..10876
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11061..11105
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
       61 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      121 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
      181 ttattacagg gacagcagag atccagtttg gttagtaccg ggcccgctct agccctagat
      241 ttaacccaac agaaggctcg acgcgtggcc tccgcgccgg gttttggcgc ctcccgcggg
      301 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
      361 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
      421 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
      481 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
      541 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
      601 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
      661 cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt
      721 gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg
      781 ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg
      841 gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg
      901 gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat
      961 gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt
     1021 ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct
     1081 ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag
     1141 ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt
     1201 cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat
     1261 aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc
     1321 tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac
     1381 cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt
     1441 ccgctaaatt ctggccgttt ttggcttttt tgttagacgc tagactgccg gatcgaattg
     1501 tcgacgaatt cggatccaga tctgccacca tggagcagaa gctgatctct gaggaggatc
     1561 tgggaggcgc tcgaggttat caaacaagtt tgtacaaaaa agttggcatg gccggcgaag
     1621 gagatcagca ggacgctgcg cacaacatgg gcaaccacct gccgctcctg cctgagagtg
     1681 aggaagaaga tgaaatggaa gttgaagacc aggatagtaa agaagccaaa aaaccaaaca
     1741 tcataaattt tgacaccagt ctgccgacat cacatacata cctaggtgct gatatggaag
     1801 aatttcatgg caggactttg cacgatgacg acagctgtca ggtgattcca gttcttccac
     1861 aagtgatgat gatcctgatt cccggacaga cattacctct tcagcttttt caccctcaag
     1921 aagtcagtat ggtgcggaat ttaattcaga aagatagaac ctttgctgtt cttgcataca
     1981 gcaatgtaca ggaaagggaa gcacagtttg gaacaacagc agagatatat gcctatcgag
     2041 aagaacagga ttttggaatt gagatagtga aagtgaaagc aattggaaga caaaggttca
     2101 aagtccttga gctaagaaca cagtcagatg gaatccagca agctaaagtg caaattcttc
     2161 ccgaatgtgt gttgccttca accatgtctg cagttcaatt agaatccctc aataagtgcc
     2221 agatatttcc ttcaaaacct gtctcaagag aagaccaatg ttcatataaa tggtggcaga
     2281 aataccagaa gagaaagttt cattgtgcaa atctaacttc atggcctcgc tggctgtatt
     2341 ccttatatga tgctgagacc ttaatggaca gaatcaagaa acagctacgt gaatgggatg
     2401 aaaatctaaa agatgattct cttccttcaa atccaataga tttttcttac agagtagctg
     2461 cttgtcttcc tattgatgat gtattgagaa ttcagctcct taaaattggc agtgctatcc
     2521 agcgacttcg ctgtgaatta gacattatga ataaatgtac ttccctttgc tgtaaacaat
     2581 gtcaagaaac agaaataaca accaaaaatg aaatattcag tttatcctta tgtgggccga
     2641 tggcagctta tgtgaatcct catggatatg tgcatgagac acttactgtg tataaggctt
     2701 gcaacttgaa tctgataggc cggccttcta cagaacacag ctggtttcct ggggctgccg
     2761 cgactgttgc ccagtgtaag atctgtgcaa gccatattgg atggaagttt acggccacca
     2821 aaaaagacat gtcacctcaa aaattttggg gcttaacgcg atctgctctg ttgcccacga
     2881 tcccagacac tgaagatgaa ataagtccag acaaagtaat actttgcttg taagacccag
     2941 ctttcttgta caaagtggtt cgataacgaa ttccgccccc cccccctaac gttactggcc
     3001 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     3061 cgtcttttgg caatgtgagg gccgcgttta acgaattcta ccgggtaggg gaggcgcttt
     3121 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     3181 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     3241 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     3301 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     3361 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     3421 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     3481 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     3541 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     3601 cgacctgcat cccgccacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     3661 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     3721 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     3781 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     3841 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     3901 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     3961 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     4021 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     4081 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     4141 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     4201 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     4261 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     4321 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     4381 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     4441 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     4501 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     4561 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     4621 cactcgtccg agggcaaagg aatagtgtac aagtaggaat tcgtcgaggg acctaataac
     4681 ttcgtatagc atacattata cgaagttata catgtttaag ggttccggtt ccactaggta
     4741 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     4801 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     4861 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     4921 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4981 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5041 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5101 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5161 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5221 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5281 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5341 gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat
     5401 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     5461 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     5521 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5581 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5641 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5701 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5761 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     5821 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     5881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6001 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6061 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     6181 cgccctatag tgagtcgtat tagcatgtga gcaaaaggcc agcataaatc aaaagaatag
     6241 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     6301 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     6361 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     6421 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     6481 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     6541 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     6601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
     6721 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6781 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6841 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6901 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6961 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7021 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
     7081 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     7141 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     7201 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     7261 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     7321 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     7381 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     7441 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     7501 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     7561 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     7621 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     7681 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     7741 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     7801 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     7861 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     7921 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     7981 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     8041 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     8101 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     8161 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     8221 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     8281 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     8341 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8401 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8461 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8521 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8581 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8641 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8701 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8761 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8821 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8881 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8941 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9001 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9061 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9121 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9181 attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg
     9241 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     9301 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     9361 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     9421 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     9481 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     9541 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     9601 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     9661 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     9721 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     9781 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     9841 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9901 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9961 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    10021 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    10081 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    10141 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    10201 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    10261 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    10321 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    10381 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    10441 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
    10501 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    10561 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    10621 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    10681 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    10741 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    10801 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    10861 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    10921 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    10981 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    11041 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    11101 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    11161 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
    11221 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
    11281 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
    11341 tagtg
//
