LOCUS       sequence-210693-       13639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13639)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU30.2-2.2
FEATURES             Location/Qualifiers
     source          1..13639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1..17)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      1396..1695
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4328..4548
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4549..5352
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     regulatory      6739..6748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9789..9809)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9789..9807)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(10146..10734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10226..10245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10905..11696,11697..11765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11528..11547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11766..11870)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11897..13239
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12860..12907)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13280..13298
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatccactag ttctagatta attaatatct aaaaattgcc ttatgatccg tctctccggt
       61 tacagcctgt gtaactgatt aatcctgcct ttctaatcac cattctaatg ttttaattaa
      121 gggattttgt cttcattaac ggctttcgct cataaaaatg ttatgacgtt ttgcccgcag
      181 gcgggaaacc atccacttca cgagactgat ctcctctgcc ggaacaccgg gcatctccaa
      241 cttataagtt ggagaaataa gagaatttca gattgagaga atgaaaaaaa aaaaaaaaaa
      301 aaaggcagag gagagcatag aaatggggtt cactttttgg taaagctata gcatgcctat
      361 cacatataaa tagagtgcca gtagcgactt ttttcacact cgaaatactc ttactactgc
      421 tctcttgttg tttttatcac ttcttgtttc ttcttggtaa atagaatatc aagctacaaa
      481 aagcatacaa tcaactatca actattaact atatcgtaat acacaatgaa gatcctctgc
      541 cttcacggta gaggctccaa caacgagatc tttcgggcgc agacggcagc cttccgtgca
      601 gatctcgatg actttgagtg gacctttgtg caaggcacgg ttcgacatac cgaaggcaac
      661 tggtccctgt acacagcaca attctcgcat cttccacttt acacatacta caaccctttt
      721 gatcctgctt ctatcttgca gactcatgac gaccttgcgc ggattatagt cgaagagggc
      781 ccattcgatg gtattctggg ctattcaggc ggtgctgcgt tagcagcaga gatcttggct
      841 caggcaacct atgatgatcc ctttgccatg gaacgaccgt ttcgatttgc agtcttcatc
      901 aacggagcat ctcccctgcg ctgttttgct atgaaggacg cccaagtcat cgaggacatc
      961 gtcgtagatt ccgcacccat gaccgagcag gcagagaata tgtttcttcg tccgagtgcg
     1021 ctgcgaaaga aggacggagt cagcgaacag gaccaggtcg accatgcaaa actactgtcg
     1081 ttgctcaaga aactcaaggg caagaagttg gtcgatggaa caacgtttct atccaacgga
     1141 acgcatggcc tttgtagatg ggaactaccc gcagacggtg cgccactgat tgacttgccc
     1201 acgctgcaca tccggagcac cgagagcgac gagagtgatc cccacgaggg tctccatttg
     1261 ctacgactct gtgatcccgg ccaagcacgc gagttccatc atggcagtgg gcatgatttc
     1321 cccagaggtc gtaaggaaat gaagcacatt gcgagactga ttcgagaggt cgctgaagct
     1381 acaacaagtc tataagcgga tctcttatgt ctttacgatt tatagttttc attatcaagt
     1441 atgcctatat tagtatatag catctttaga tgacagtgtt cgaagtttca cgaataaaag
     1501 ataatattct actttttgct cccaccgcgt ttgctagcac gagtgaacac catccctcgc
     1561 ctgtgagttg tacccattcc tctaaactgt agacatggta gcttcagcag tgttcgttat
     1621 gtacggcatc ctccaacaaa cagtcggtta tagtttgtcc tgctcctctg aatcgtctcc
     1681 ctcgatattt ctcattttcc ttcgcatgcc agcattgaaa tgatcgaagt tcaatgatga
     1741 aacggtaatt cttctgtcat ttactcatct catctcatca agttatataa ttctatacgg
     1801 atgtaatttt tcacttttcg tcttgacgtc caccctataa tttcaattat tgaaccctca
     1861 cgatccagtt ctccagtgac acagccttta tctggtcaaa cctttctttc taatcaccta
     1921 tgctgatgct taattaaggg atttttgtct ccatcaacgg catgcgccca aaaatgacgt
     1981 tttttttaac ccatagacac gaaactaccc attttccacc ggcctgacct accaccggaa
     2041 caacggccat ctccaacttg caagttgggg aaattaagag catcgcaggt ttaatggaag
     2101 aaaaaaaaaa ggtacagcac agcgcaaatg gagttagttc ccttatgtca cacactcaca
     2161 cacagtcggt cagatcaagc atactgggtg cgtataaata gagtggccat tgccaccctg
     2221 tttatctcaa aatctgtctt gttagtggtc ttctcccttt ttcaggttac aattctcttg
     2281 tttctactta gtatataagt atatcaagct atattaagca tactatcaac tgtcaactct
     2341 atcctcaaaa tacaatacaa aatggctacc atgagcgacg tctgggttgg cctgccatgg
     2401 ccagtacaat tgatcgcgac ctgctttgtc ctcactgcaa ctctgttgat cacgactcgc
     2461 ctcaattcac tctcccattc atggccagcg gcagcaggaa aaggccacga acctccaata
     2521 gcgccctact ggcttccagg aatccagcat ctggcagcct tccttctcga tcctgcccac
     2581 actttccgca cgacccagtc gaaatacaaa gattcgcctt acacattgct catggggaac
     2641 gtgaagttcc acgtttttgg ttctccatct gccgcgaccc atgtctttgc acgctcgcgc
     2701 acgtttgcgt atgaaccggt gaccttgagc atgctggaga atggactgga tctgcccgtg
     2761 gccgaccgag tttactttca aatcggtctg gatcgagcaa atagtgcaga gcatgcgaag
     2821 gggttcgtgg tgcaaaacca taatgtctgg ttgcgatact tgtcaggcga gccgttggac
     2881 gacctgatgc agctattcac acgccacttc caccaaatcc tggagcaaca cctggatatg
     2941 aagacaagag gctggcagac agtcgatcta taccaagtcc tgcgcaaagt cattttcgac
     3001 acctctgtcc tgacgttctt cggtccccat cttgctcaga tatggggtcc taccatgtgg
     3061 aaggacttct gcctgttcaa cgatgccacc tatattggtg tcagaacaaa tttggcgtat
     3121 gtcttgcagc cgcgcgctgg ccgcgcgcgg gagagaatgc tacgcgcctt tgaacagtgg
     3181 ttaacgcacc attcagaggg agactggccg gacaaggaca aatattggaa cgaggaatgg
     3241 ggcgcaaaaa tgaattggga acgtgaccat ctggcgcgac aattcggcct ctccctccgt
     3301 ggacgggcgg ccatgcaggc gagcttcctg tttgcaatcg ttctgaacgc cgcgccgatg
     3361 agttcctggt ttacctgggt ggcggcgagt aattcagatc gcctggcaaa gtgtcgagtc
     3421 gagtcgtcca agttcttgcg accgagcaaa tcaagccgct tcgatcacga attggacatc
     3481 gacgccacgc aaatgagagg cagcaacttc atgcaggcgt tgtggaagga agcgttacgc
     3541 ataggcacgg cagcctcgcc agcacgtgta gtcatggaag acacagagct ggaagggtat
     3601 gtcgtaagga agggcagtgt gatcctcatg cccactgcgt tgctccacca tagcgacttg
     3661 ttcccgagag cggaccaggt tgacacggaa aggtggacag gcgaggatgc catagtccaa
     3721 cagcggagta aaagtattcg cacatttggt ggaggtatgg gcatgtgttc tggacggcat
     3781 gcggccgagc aggaggtcat cggtctggtc tcgaagctta ttacgctgtt tgatatcgag
     3841 ttcgaagact cgtggaagaa caagttccac ttcgacccaa gaagtctcgg aattatgcat
     3901 cctgcaggtc ctctaatggt acgcctgaag cggagagtat tatgaacaaa tcgctcttaa
     3961 atatatacct aaagaacatt aaagctatat tataagcaaa gatacgtaaa ttttgcttat
     4021 attattatac acatatcata tttctatatt tttaagattt ggttatataa tgtacgtaat
     4081 gcaaaggaaa taaattttat acattattga acagcgtcca agtaactaca ttatgtgcac
     4141 taatagttta gcgtcgtgaa gactttattg tgtcgcgaaa agtaaaaatt ttaaaaatta
     4201 gagcaccttg aacttgcgaa aaaggttctc atcaactgtt taaaaggagg atatcaggtc
     4261 ctatttctga caaacaatat acaaatttag tttcaaagat gaatcagtgc gcgaaggaca
     4321 taactcattc aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttcct
     4381 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
     4441 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
     4501 ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct
     4561 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
     4621 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
     4681 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
     4741 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
     4801 aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag
     4861 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
     4921 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
     4981 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
     5041 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
     5101 caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg
     5161 ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc
     5221 tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct
     5281 aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc
     5341 cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
     5401 gcttcaattt aattatatca gttattaccc tatgcggtgt gaaataccgc acagatgcgt
     5461 aaggagaaaa taccgcatca ggataggaaa aaaccgagct tcctttcatc cggcgcggct
     5521 gtgttctaca tatcactgaa gctccgggta ttttaagtta tacaagggaa agatgccggc
     5581 tagactagca agttttaggc tgcttaacat tatggatagg cggataaagg gcccaaacag
     5641 gattgtaaag cttagacgct tctggttgga caatggtacg tttgtgtatt aagtaaggct
     5701 tggctgggga tagcaacatt gggcagagta tagaagacca caaaaaaaag gtatataagg
     5761 gcagagaagt ctttgtaatg tgtgtaactt ctcttccatg tgtaatcagt atttctactt
     5821 acttcttaaa tatacagaag taagacagat aaccaacagc ctttcccaga tatacatata
     5881 tatctttatt tcagcttaaa caataattat atttgtttaa ctcaaaaata aaaaaaaaaa
     5941 accaaactca cgcaactaat tattccataa taaaataaca acatgctctc ttccgaccgc
     6001 acccaatata ctctggcact ctggctgacg cccgtcgtcc tctatgtcgt gtacgcgtgg
     6061 ctcaaagtca tcaccggcgc cttctttggc cctttgagca aaattcccgg ccccaagctc
     6121 tgggccatct cgcgccttcc tcgtatgtac atggaatggc agggtgagga agcagctgga
     6181 gtcgccgcgt tgcacaagaa gtacggcccg gtcgtgcggc tggcgcccaa cgaagtgtcg
     6241 tttgctggtg gtgctcaggc ttggaaagac atttatggct accgaaagaa cgtgggccaa
     6301 gagaagcatc catatcgttc gtcggcgtcg tattggcctc cggccaacgg agccccgagc
     6361 atgattcagg ccatccgtga cgaagaccac cagcgactgc gcaagagtct ggccgtctcg
     6421 ttcagtgaga agtccatgaa gctgatggaa ccacgcgtca agggctgggc tcggcttctg
     6481 cagaccaagt tggctgagcg cggtgatgct gcggtggata tggtcgagtt gtttagatgt
     6541 gcaactttcg atgccatggg tgacctcgcg ctaggggacg acctgcaaat gctgagaaat
     6601 ggccgtctct ccgactacgt ccatgcagct tttagtggca ttcgaaccat catccgcctt
     6661 cgggtcctct ccacgtacaa ttccttcacc cgctggctcg ttcaagactg tttcttccaa
     6721 agcgcattcg tccggaaggc cgccatggaa aatcttcgct actccgcgga ccgtgtggat
     6781 cgtcgcctgc aagaaccaga ccgagaccct cccgatctct ggacaaaacc cctgactggt
     6841 atcgacggca agatgtccct ggacgaatat cactccaccg cacagcttct catgactgcg
     6901 ggcaccgaca cttcggcgat aggcttgtcc ggcacactat attatcttct taccaacccc
     6961 gactgcctcg ccaaggtcac tgacgaggtt cgctccgcct tctcatctat ggacgacgta
     7021 acctacgaaa ccctcgccca actgaagtac ttggacgccg cgctgcaaga aggcctgaga
     7081 ctctacccac cggtgcctac cggcgcacct cgcgtcactc cgatgcctgg atggtccatc
     7141 ggcggctact ggatcccagg tggtgtgaac gtccacgcgc cccactatgc cacctctcgc
     7201 cttcccgccc acttcaccgc tcccgaatct ttccacccag agcgctggct gggcgacgag
     7261 aaattccaaa acgaccggct tgatgccgtc cagccgttca acctcggccc tcagaactgc
     7321 ttgggtcagg cttttgccat gaacgagatg cgcctcttca tggccgctgt gttgctccaa
     7381 ttcgatctac agttgtgcga ggacccccga gcctggttgg atcaacgagt gttctccatg
     7441 tgggagacga agccgttgat gtgtgtcgcg accaagacga gctgaagtgc ttttaactaa
     7501 gaattattag tcttttctgc ttattttttc atcatagttt agaacacttt atattaacga
     7561 atagtttatg aatctattta ggtttaaaaa ttgatacagt tttataagtt actttttcaa
     7621 agactcgtgc tgtctattgc ataatgcact ggaaggagaa aaaaaaggtg cacacgcgtg
     7681 gctttttctt gaatttgcag tttgaaaaat aactacatgg atgataagaa aacatggagt
     7741 acagtcactt tgagaacctt caatcagctg gtaacgtctt cgttaattgg atactcaaaa
     7801 aagatggata gcatgaatca caagatggaa ggaaatgcgg gccacgacca cagtgatatg
     7861 catatgggag atggagatga tacctccatt gggccgatga agttagtcga cggatagaag
     7921 cggttgtccc ctttcccggc gagccggcag tcgggccgag gttcggataa attttgtatt
     7981 gtgttttgat tctgtcatga gtattactta tgttctcttt aggtaacccc aggttaatca
     8041 atcacagttt cataccggct agtattcaaa ttatgacttt tcttctgcag tgtcagcctt
     8101 acgacgatta tctatgagct ttgaatatag tttgccgtga ttcgtatctt taattggata
     8161 ataaaatgcg aaggatcgat gacccttatt attatttttc tacactggct accgatttaa
     8221 ctcatcttct tgaaagtata taagtaacag taaaatatac cgtacttctg ctaatgttat
     8281 ttgtccctta tttttctttt cttgtcttat gctatagtac ctaagaataa cgactattgt
     8341 tttgaactaa acaaagtagt aaaagcacat aaaagaatta agaaaatgtc ccacaacggc
     8401 gagcccgacc cgctctaccg cgagccctat ccccttaacc gcgacttgct cgcctccgcc
     8461 cgcctgcacc tccagcacgc cgtcttcgtc aacggtctag gctacctgct gcacccggcc
     8521 gtccctatcg ggcccgcctc gcacgtggct gacatcgcca ccggcaccgg catcttcgcc
     8581 ctcgccctcg ccgcggccca ccccgaagcc cgcgtcgaag cctccgacat ctccctcgcc
     8641 caggtccccg cccagcactg gtggcccgcc aatgtgacct ttgcggtcct cgacatcttc
     8701 caggacccct tgccggcctc actgctcgcg cgctacgacg tcctctgcct ccgccacttc
     8761 gtctccctgc agcccggcga tccgcgcccg ctgatcgccc gcttgctggc catgctgcgg
     8821 cccgggggct atctgcactg gcaggaatgg gacctgacca ccgccgccgt cttcgcccca
     8881 gacggcacct ccacccctaa gatgcaggcg ctgatcgatt acatcaaaca tccctcccga
     8941 cacaccgccc aggccacctg ggttggcgac ttgcatcaga gggtcgccgc cggcgaggtg
     9001 cctggcgtgg agctggtcgc tcacgaccgt gtccgcgccg ctaaggccgc cgtcgtcgct
     9061 atgcagcagg aactcatggc gctgggcacc aaggaggtag tcgccagtct gcgggcccgc
     9121 gagggcgaag cgagcgtcga ggccgctaag ttcgagaaga tcgcgcacga cgccgtcgac
     9181 gagtgtctga gcttggggag gggccctgtg atcgatcagg agatggtgac gtgggtgctg
     9241 aggaagaagt gaggagattg ataagacttt tctagttgca tatcttttat atttaaatct
     9301 tatctattag ttaatttttt gtaatttatc cttatatata gtctggttat tctaaaatat
     9361 catttcagta tctaaaaatt cccctctttt ttcagttata tcttaacagg cgacagtcca
     9421 aatgttgatt tatcccagtc cgattcatca gggttgtgaa gcattttgtc aatggtcgaa
     9481 atcacatcag taatagtgcc tcttacttgc ctcatagaat ttctttctct taacgtcacc
     9541 gtttggtctt ttatagtttc gaaatctatg gtgataccaa atggtgttcc caattcatcg
     9601 ttacgggcgt attttttacc aattgaagta ttggaatcgt caattttaaa gtatatctct
     9661 cttttacgta aagcctgcga gatcctctta agtatagcgg ggaagccatc gttattcgat
     9721 attgtcgtaa caaatacttt gatcggcgct atgcggccgc caccgcggtg gagctccagc
     9781 ttttgttccc tttagtgagg gttaattgcg cgcttggcga gactcaaaca agatatattt
     9841 tgcccgcctt acagacgaaa ctagttggag gttatggagc atactatcac gtgggcggcc
     9901 actggtgagt tactacaccc caggggcaac gttgatgctc ctaaaaaact ctggctggac
     9961 gcaagccgta acacccgtgt cacttcataa tcgtttgcaa ttcagggctt gatctacaca
    10021 ttaccctgtt atccctatta attaatacgg ttatccacag aatcagggga taacgcagga
    10081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10561 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    10621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10681 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    11041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11881 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
    11941 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
    12001 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
    12061 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
    12121 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
    12181 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
    12241 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
    12301 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
    12361 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
    12421 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
    12481 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
    12541 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
    12601 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
    12661 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
    12721 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
    12781 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
    12841 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
    12901 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
    12961 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
    13021 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
    13081 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
    13141 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
    13201 ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa ccattattat
    13261 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
    13321 tgatgacggt gagcgcgttt cggtgatgac ggtgaaaacc tcggcgcgcc attaccctgt
    13381 tatccctaat atacaacgca aagaagctgg aaaattggcg agggaatcct gtttctgtct
    13441 atccaagaat gggcatgagg tggcaaccgt cgtgctagcg tacagggtgc actttgtaac
    13501 catttgggac accggacact cgctgttttc gaaattaccc tttaagcgcg ggtattgaac
    13561 caggcttatg cccaagatcg tagcaagcat ttaaatgacg gtatcgataa gcttgatatc
    13621 gaattcctgc agcccgggg
//
