LOCUS       sequence-210700-       12022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12022)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU67.1-2.2
FEATURES             Location/Qualifiers
     source          1..12022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(264..1358)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1359..1766)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(2061..2079)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2120..3462
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    2452..2499
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        3489..3593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3594..3662,3663..4454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3812..3831)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4625..5213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5114..5133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5550..5570
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5552..5570
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(11489..11512)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctagaacta gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc
       61 atttcgttga gccattagta tcaatttgct tacctgtatt cctttacatc ctcctttttc
      121 tccttcttga taaatgtatg tagattgcgt atatagtttc gtctacccta tgaacatatt
      181 ccattttgta atttcgtgtc gtttctatta tgaatttcat ttataaagtt tatgtacaaa
      241 tatcataaaa aaagagaatc tttttaagca aggattttct taacttcttc ggcgacagca
      301 tcaccgactt cggtggtact gttggaacca cctaaatcac cagttctgat acctgcatcc
      361 aaaacctttt taactgcatc ttcaatggcc ttaccttctt caggcaagtt caatgacaat
      421 ttcaacatca ttgcagcaga caagatagtg gcgatagggt tgaccttatt ctttggcaaa
      481 tctggagcag aaccgtggca tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa
      541 gaggccaagg acgcagatgg caacaaaccc aaggaacctg ggataacgga ggcttcatcg
      601 gagatgatat caccaaacat gttgctggtg attataatac catttaggtg ggttgggttc
      661 ttaactagga tcatggcggc agaatcaatc aattgatgtt gaaccttcaa tgtagggaat
      721 tcgttcttga tggtttcctc cacagttttt ctccataatc ttgaagaggc caaaacatta
      781 gctttatcca aggaccaaat aggcaatggt ggctcatgtt gtagggccat gaaagcggcc
      841 attcttgtga ttctttgcac ttctggaacg gtgtattgtt cactatccca agcgacacca
      901 tcaccatcgt cttcctttct cttaccaaag taaatacctc ccactaattc tctgacaaca
      961 acgaagtcag tacctttagc aaattgtggc ttgattggag ataagtctaa aagagagtcg
     1021 gatgcaaagt tacatggtct taagttggcg tacaattgaa gttctttacg gatttttagt
     1081 aaaccttgtt caggtctaac actaccggta ccccatttag gaccacccac agcacctaac
     1141 aaaacggcat cagccttctt ggaggcttcc agcgcctcat ctggaagtgg aacacctgta
     1201 gcatcgatag cagcaccacc aattaaatga ttttcgaaat cgaacttgac attggaacga
     1261 acatcagaaa tagctttaag aaccttaatg gcttcggctg tgatttcttg accaacgtgg
     1321 tcacctggca aaacgacgat cttcttaggg gcagacatta gaatggtata tccttgaaat
     1381 atatatatat atattgctga aatgtaaaag gtaagaaaag ttagaaagta agacgattgc
     1441 taaccaccta ttggaaaaaa caataggtcc ttaaataata ttgtcaactt caagtattgt
     1501 gatgcaagca tttagtcatg aacgcttctc tattctatat gaaaagccgg ttccggcgct
     1561 ctcacctttc ctttttctcc caatttttca gttgaaaaag gtatatgcgt caggcgacct
     1621 ctgaaattaa caaaaaattt ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg
     1681 tgttctcgtt atgttgagga aaaaaataat ggttgctaag agattcgaac tcttgcatct
     1741 tacgatacct gagtattccc acagttaaat gcttgctacg atcttgggca taagcctggt
     1801 tcaatacccg cgcttaaagg gtaatttcga aaacagcgag tgtccggtgt cccaaatggt
     1861 tacaaagtgc accctgtacg ctagcacgac ggttgccacc tcatgcccat tcttggatag
     1921 acagaaacag gattccctcg ccaattttcc agcttctttg cgttgtatat tagggataac
     1981 agggtaatgg cgcgccgagg ttttcaccgt catcaccgaa acgcgctcac cgtcatcacc
     2041 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     2101 aataatggtt tcttagtatg atccaatatc aaaggaaatg atagcattga aggatgagac
     2161 taatccaatt gaggagtggc agcatataga acagctaaag ggtagtgctg aaggaagcat
     2221 acgatacccc gcatggaatg ggataatatc acaggaggta ctagactacc tttcatccta
     2281 cataaataga cgcatataag tacgcattta agcataaaca cgcactatgc cgttcttctc
     2341 atgtatatat atatacaggc aacacgcaga tataggtgcg acgtgaacag tgagctgtat
     2401 gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt tgaagttcct
     2461 attccgaagt tcctattctc tagaaagtat aggaacttca gagcgctttt gaaaaccaaa
     2521 agcgctctga agacgcactt tcaaaaaacc aaaaacgcac cggactgtaa cgagctacta
     2581 aaatattgcg aataccgctt ccacaaacat tgctcaaaag tatctctttg ctatatatct
     2641 ctgtgctata tccctatata acctacccat ccacctttcg ctccttgaac ttgcatctaa
     2701 actcgacctc tacatttttt atgtttatct ctagtattac tctttagaca aaaaaattgt
     2761 agtaagaact attcatagag tgaatcgaaa acaatacgaa aatgtaaaca tttcctatac
     2821 gtagtatata gagacaaaat agaagaaacc gttcataatt ttctgaccaa tgaagaatca
     2881 tcaacgctat cactttctgt tcacaaagta tgcgcaatcc acatcggtat agaatataat
     2941 cggggatgcc tttatcttga aaaaatgcac ccgcagcttc gctagtaatc agtaaacgcg
     3001 ggaagtggag tcaggctttt tttatggaag agaaaataga caccaaagta gccttcttct
     3061 aaccttaacg gacctacagt gcaaaaagtt atcaagagac tgcattatag agcgcacaaa
     3121 ggagaaaaaa agtaatctaa gatgctttgt tagaaaaata gcgctctcgg gatgcatttt
     3181 tgtagaacaa aaaagaagta tagattcttt gttggtaaaa tagcgctctc gcgttgcatt
     3241 tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc
     3301 atttttgttt tacaaaaatg aagcacagat tcttcgttgg taaaatagcg ctttcgcgtt
     3361 gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc
     3421 gttgcatttt tgttctacaa aatgaagcac agatgcttcg ttcaggtggc acttttcggg
     3481 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3541 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3601 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3661 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3721 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3781 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3841 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3901 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3961 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4021 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4081 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4141 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4201 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4261 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4321 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4381 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4441 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4501 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4561 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4621 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4681 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4741 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     4801 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4861 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4921 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4981 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5041 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5101 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5161 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5221 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5281 ctgcgttatc ccctgattct gtggataacc gtattaatta atagggataa cagggtaatg
     5341 tgtagatcaa gccctgaatt gcaaacgatt atgaagtgac acgggtgtta cggcttgcgt
     5401 ccagccagag ttttttagga gcatcaacgt tgcccctggg gtgtagtaac tcaccagtgg
     5461 ccgcccacgt gatagtatgc tccataacct ccaactagtt tcgtctgtaa ggcgggcaaa
     5521 atatatcttg tttgagtctc gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     5581 tggagctcca ccgcggtggc ggccgcatag cgccgatcaa agtatttgtt acgacaatat
     5641 cgaataacga tggcttcccc gctatactta agaggatctc gcaggcttta cgtaaaagag
     5701 agatatactt taaaattgac gattccaata cttcaattgg taaaaaatac gcccgtaacg
     5761 atgaattggg aacaccattt ggtatcacca tagatttcga aactataaaa gaccaaacgg
     5821 tgacgttaag agaaagaaat tctatgaggc aagtaagagg cactattact gatgtgattt
     5881 cgaccattga caaaatgctt cacaaccctg atgaatcgga ctgggataaa tcaacatttg
     5941 gactgtcgcc tgttaagata taactgaaaa aagaggggaa tttttagata ctgaaatgat
     6001 attttagaat aaccagacta tatataagga taaattacaa aaaattaact aatagataag
     6061 atttaaatat aaaagatatg caactagaaa agtcttatca atctccctag ccgtaatact
     6121 ccgccagcca cttgcacagg tccccgacgg tagggtagtc cacgaaaagg ccaccaccaa
     6181 ccttgatatc aagttcgact ctgaatttct ccgcgagcac cagcgacatg agactgtcga
     6241 cgcccaggtc cgcgaaccgg acatcatctt ccaggtcagc aatgtccaac gcgccctcat
     6301 tcgcaatgag ctcaagggct ttgctgacga tgctgttatc atcacttgac gtctcggatt
     6361 cgctactgct cggcgagact tccacaacaa cagtggtggc ggctttgaag tccttcggcg
     6421 gtccagggac ggcagtcgga tggatgacat cgtcagcact gaagcgtgtc ggctttggca
     6481 gttgctgctg cgtggtagtc gctggctttc cgtgggctgc ggctgggagt agggcttgcg
     6541 gagctgcaac tttcaccgct ttgtcctcca tcgccgccat cttatctggt ggggaaaaga
     6601 atcggctgag caagatccgc gggtagcggc ggaattggat gccacccacc atgcccatga
     6661 tggcgttgtc ttgcatgatg tacacgtcgc ccaagaagat ggtggggtcc tcagcagtgg
     6721 ggatcatctt gacgtaggac cggtactttg ctcccgggat gagaggcttt gcaaattgca
     6781 tcgacttcca tccaggtgtg acgcagtagt tgttccggac atccatcgcg tcggagcagt
     6841 tcatgatgaa tccagccaga tgggccacgc tgtcgatgaa atagggcgcc acggtccagg
     6901 tgccgctttc cttggtagtc agctgcacgt cggcaaatgc ctccagatcg tgcatcacga
     6961 cggattgcat gccgcgatac ttgtcggcat agtcgacgag attggcggcg aagagtgtgt
     7021 atgccatgtt ccgcgaaaat cggctggctt ggccttttgc cgccatctgc tccagcgcct
     7081 cgatccggct gtgaatcaag tgcgtcagag ggagccagga gctgagccac gcagaagcat
     7141 cgccgtagac gatcttggcc gtggcaaatg gctcatgcac gtcaccggcg ttgtcaacgt
     7201 tttgccaact caattcaaca acaccggatt cgatgttggc cgtggcggcg gtgacctgga
     7261 tcaactgagg cagcttcttg ttactttggg cgacgaggcc ctttgtgacc accaggtccg
     7321 cgatgttcat gttgacgtcc tttgacttgg gatagaattt gcggtacaaa tagctaccga
     7381 gggtataagc gatatcggca tggatggacg atgtgacaac gccgcaatcg ttcatgcgat
     7441 gaccatgagc tgcagcaaga aaatccggtt gcatgagatc ggattgcatg atcactgtac
     7501 cagcggagcc atcaacgacc tccctgacga cttgctgtac tgtcgaagtc tgcaagtcgc
     7561 tgacaggcag tggttttgag atcgtctgtc ctgttgcagc cgatttctcg gccgcgtaaa
     7621 aagtattgcc cttggtcaaa caccagtcgc cgttgtactg gagccagaag ttcttctcgt
     7681 tccaggcgta tgtaggcaag tctaggaggc ggagtctgct ttcgaagggg tgatgaaact
     7741 cgttccagcc aacggtgaca cccgcgacgt gcaatgtggc tattgcttct gccatggtag
     7801 cccagttgtc ctcgccgcgc cggaaggagg ggacggcgac ggctgtggat gggatggagg
     7861 ttttgacaaa attggtgcaa acaggatgag ggccaatctc tacccagata gtctcgtcat
     7921 tgatggtcga aacctcttgt gcgttctcca gtgcagggaa gaatctcacg gcttctctcg
     7981 tggctcggcg gacgtagttc gcattgaagg tcttgtcgtc gaaaacgact ctgccgagca
     8041 agggcgagat gatggggact ttgggctcct ggaaaatcac acccgtcttc accgttgcct
     8101 cgaactcgtc caggataggg tcagtctgtt cagaatggaa tgcaaacgcc acgtcgagct
     8161 taatgcagcg caatccagcc ttctcgagtg gcacagcgac ctcatccatc tgctgcttgg
     8221 tgccactgag cactgtgtcc gaaggtccat tgacacaagc aacggtatac ggcttattcg
     8281 tcgagttctg ggcaatggtg gcaagagagg ctcggacggc catcatggtg tggcttccaa
     8341 ttgtgcattt ttcttgcagc atctgggccc ggcgtcctac catgaatatg gtgtcagcgg
     8401 cagagacgac gccggccacg tgcattgcgg cgtattcgcc caaactgtga ccgaggacga
     8461 catccggctt gacgcccaga gcagcccagt atttagccag cgccatctcg gagcacacca
     8521 gagcaagctg agtcacaacc ggggaatgtg catgatcctt cggatgtgat ccgtcgagag
     8581 ccggaatgaa agatggaaag ccttggcctt gggccagatg atccaaatgc tgaatatgtt
     8641 cgcggaagat cggaacgtcg cggtacaatt cgaggtccat ggacttgttc gaagcgcctt
     8701 ggccggtgaa ggtgaaagca atcaatggtg gatcggactg tgccagggcc ttgacggagt
     8761 caatcttcgc cagacgggaa gtgagctgtg actttaaatg agcggcgtcc ggggcggtca
     8821 ctgtcagacg gttgcggtag tggtagcgac gtgcggtggt cgtgtatgag agatcagcga
     8881 ggttgagctg tggattggca tcgatgtaag cgatcaggcg ctcaatgttt cctgccaatg
     8941 atgccttgct tttcgccgac acggcaatga cctgggtagg gcgtggatct ctccctgttg
     9001 ttgtgcgagt cggtgcctct tccaaaatca tggttgtatt gccaccagcg gcaccaaagt
     9061 tgttgagagc aacgacacgc tttgtgctgg gtttctgctt ccactgtgtc atctcgaacg
     9121 ggatatggac gttgcgcttg tcaaaatcct tgggaagacg agggttgatc tccgttttaa
     9181 tgccgatatg tggtggaatg ccgttcttct gcaacatcaa gagcaccttg atcaaagctg
     9241 tcgtccccgc aacggactcg ccatgaccga catttgcctt gacagcgcca atgtgcagag
     9301 gctgatcctt gctgcgccgc ttgacgagcg gagcgtacac gtccattatg ccttgcatct
     9361 cctcgtagtc gccagcctgg gtgccggtgc catggagctc aacataagaa acatccaatg
     9421 gatcaacacc agattgacga agcacctgtc gggccaagta tgcttgatga ccggcgtgag
     9481 gatgagtgat agaaacagcc tcggcagaat gattggttcc ggcacccagt attactccca
     9541 agatgttgtc gttgtcagcc tctgcgtctt gaagccgttt gatgacgaca gatcccacac
     9601 cgtctgcgcg acagtatcca tcagcggtgg cgtcccaggt tttgcatgca ttatgccctt
     9661 tggtcaggaa gtgaccgtgg cccaggccag caaagccatc aggatttgtg agcacattga
     9721 cgccgcccgc gacagccata tcgacatcac cttgccaaag cgcttgacaa gcgacctcaa
     9781 tagccgccag tccagacgag caggccgtat caatgctgta actagggcca gcaaacttga
     9841 agaagtagtt gatgcgacca ggaccgaatg cacgacaacc tcctggaata tagtaagtgc
     9901 tgacctcttg cccttggttg acctctcgat aatcgtcagc ggcctggccg tagtaggtgc
     9961 cgatacgttc tagcttggtg gacgaagtgc ggttgccgac gtaaccagca cgctcgagcg
    10021 cttcgaatgc cgtgactaaa gccagacgca tctgcggatc ggcaacttgg gcttcacgtg
    10081 gagacatgtt gaagaaaggc gcgtcgaaca agccgggctc atcgatgaag catccgtact
    10141 gcgtcatgct cttgttcatt tgcttgccag tcgggtcata gtgcgtgtca atgtcgaagc
    10201 gatctgccgg gattcgtcta gagacgtcga gcccctgttc gaggatttcc cagaacttct
    10261 ctgtgttcgt agctccgccc gggagcctgc atgacatgcc cacaatggcg agcttggatt
    10321 gcgccgagcc tcgcggtgac gccgcgggaa atgcagcgtc gaaaatccat ggtactagat
    10381 tgatgatgga tgcttcgagt tgaggaaggc tggattccaa ggatgctttg aggtcgttga
    10441 gggggattga gttaccgaag gaggaaagcg ctacctggct ggcaccagca ctttggagtt
    10501 gagcaaccac gccccggatg acttggtccc agcagatcgc atgcgtgagt aactcggaga
    10561 taacacattc gaataattct cccgcattcg aggcactgaa tggcagaccg gtactggtgg
    10621 aataaattgg ggtgatggga caggattttg cgaggtttgc gttcaacgaa ctaccccaga
    10681 caatgctttg aacgtcccga gagccgtaga catgaggcgc gtggcaaagt cctccataga
    10741 cgggcagagc aatagatctg gagtcccgga agaattctga gcgactgaag agcgccttta
    10801 atctcgaggg tggtccactg actgtgactg acgttcggct gacagcactg aggaatatct
    10861 tgctggtgtc aggtatcttt tctcgcgatt ggattgcatc gagcgcctgc tgcgccgcga
    10921 caggatcgac gttgtgaatc acgtaggccc atgtctctgg agctgctgcg agatcgcgag
    10981 attccaagtt ctcggaaacg gcgagaacat ggcagcccat acggaaagcg agacgcaccg
    11041 catctgcacc agccagcggc aaatccacca cggcggagga gagcgacacg gcagtcagtg
    11101 ccagtaagcc gatgcctagg cccgccagtg atgtctgttc taatctcgcc agctcttccg
    11161 gctgattctc cacataacca atgtatgttg caagctggac gactgtgagc agaactccgt
    11221 cgacagcacc gcaaaggagc ccttcgcgca gctcgggatt ctcagcccag gtaagaatat
    11281 cttcgaacgg tgggaataac tgtttcagct ctgttgagag gtgtcggatt tcatccttga
    11341 cggctagagt agcctcatgg agaaactgag cgagaatggt atgtcgtctg tccttgctgt
    11401 ggttgtggag gcaacgaaag aggtactgga gatcctcctt cggaaactca ttgctgaaat
    11461 agaggagctt catgtttccc atactagtct tatcatcatc atccttataa tccattgtgt
    11521 attacgatat agttaatagt tgatagttga ttgtatgctt tttgtagctt gatattctat
    11581 ttaccaagaa gaaacaagaa gtgataaaaa caacaagaga gcagtagtaa gagtatttcg
    11641 agtgtgaaaa aagtcgctac tggcactcta tttatatgtg ataggcatgc tatagcttta
    11701 ccaaaaagtg aaccccattt ctatgctctc ctctgccttt tttttttttt ttttttcatt
    11761 ctctcaatct gaaattctct tatttctcca acttataagt tggagatgcc cggtgttccg
    11821 gcagaggaga tcagtctcgt gaagtggatg gtttcccgcc tgcgggcaaa acgtcataac
    11881 atttttatga gcgaaagccg ttaatgaaga caaaatccct taattaaaac attagaatgg
    11941 tgattagaaa ggcaggatta atcagttaca caggctgtaa ccggagagac ggatcataag
    12001 gcaattttta gatattaatt aa
//
