LOCUS       sequence-210703-       13624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13624)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU71.1-2.2
FEATURES             Location/Qualifiers
     source          1..13624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             511..534
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(6824..6835)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8055..8075)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8055..8073)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8412..9000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8492..8511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9171..9962,9963..10031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9794..9813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10032..10136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10163..11505
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11126..11173)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11546..11564
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11859..12266
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12267..13361
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13608..13624)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13608..13624)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttaattaata tctaaaaatt gccttatgat ccgtctctcc ggttacagcc tgtgtaactg
       61 attaatcctg cctttctaat caccattcta atgttttaat taagggattt tgtcttcatt
      121 aacggctttc gctcataaaa atgttatgac gttttgcccg caggcgggaa accatccact
      181 tcacgagact gatctcctct gccggaacac cgggcatctc caacttataa gttggagaaa
      241 taagagaatt tcagattgag agaatgaaaa aaaaaaaaaa aaaaaggcag aggagagcat
      301 agaaatgggg ttcacttttt ggtaaagcta tagcatgcct atcacatata aatagagtgc
      361 cagtagcgac ttttttcaca ctcgaaatac tcttactact gctctcttgt tgtttttatc
      421 acttcttgtt tcttcttggt aaatagaata tcaagctaca aaaagcatac aatcaactat
      481 caactattaa ctatatcgta atacacaatg gattataagg atgatgatga taagactagt
      541 atgatgcagc gacatcctac cgccgaggcc acaaccgcgg gtggctgcgt gcatcacctc
      601 tttgaacatg ctgttaaggg ctacccagac aacattgctc tgatcggtgg cacgcatgag
      661 ctatccttcc agcagcttaa cacgctcgct aaccattttg ccagtgctct ggtcaggcga
      721 ggagtagggc gcggcgatct ggtcgctata gcgctggatc gatctgtgga tctggtcgtc
      781 gccttgttag gagtccttaa gtcgggggcg gcatacgtgc ccgtagatat gggtttccct
      841 attcggcgga tcaggcagat gatggatgat gcgggcccga agtttgttat atgtcgagat
      901 agtacactgg atgcactgtc atcctggaga gatatatgcc tgagtgttga tgacatacga
      961 aacaacaacg gcgagaacca ctccttgtcc ggccctgatg gaggcaactt ggacctggac
     1021 gtgtgcgccg aggatctcgc atatatcatc tatacatcag gttcgactgg aaagcccaag
     1081 ggcgtagaaa tcagccatgg agcggtatca aatcttctct cgtcgatgcg acgtgaaccg
     1141 ggatgcgacc aaacggatcg actactagcc ataacaacca tttctttcga tatagccgtt
     1201 ctagagcttt ttctgccact cgtctgcggc gcaaagacgg tcattgccca ggcgcatcaa
     1261 gtccgcgacg cgggagctct aatcaaatta ttgaaacacc acgcaatcac catgatgcaa
     1321 ggaaccccag ccacctggca attgttgctg gattcgggct ggcaaggcaa cccccggtta
     1381 ggaaaagttt tatgtggtgg cgaggcgctt tctcgtcgac tggccgacag gctgctttct
     1441 tgcgccgact cggtatggaa catgtacggg ccaaccgaag ccactgtgtg ggcgagtgtt
     1501 tggcgggtgg gccttggtgg tgatgtgctc attgggaggc caatagataa catgcaattc
     1561 tacatactcg gcgaaggcct atcgccagtg ccgcaaggga gtcctggtga gctgtacatt
     1621 ggaggtgctg gcgtcgcccg cggctatcat cgcaaccctg agcttactcg atctcgcttc
     1681 atcgagagcc ctcgtcatgg cggcattctg taccaaacag gggacttagc ccgttgtctg
     1741 gcgcccgacg agctcgtggt gctgggccgg gtggatgacc aggtaaagat ccggggccat
     1801 cggattgagc taggcgatat cgaagcggca cttgctgccc acgaggatgt ctccaaggcc
     1861 gtggtcgtca gtcgtgccga ccaactggtc gcctactgtg tccggaaact gcctcaggtc
     1921 actgatgagg accaggcccg cagcctggca gagtggactg ccacgtggga ccatgcgtac
     1981 gaaaccacag atcaagacgc cgtgagccag gatgccatcc tcaacctggc cgggtggagg
     2041 aatagctacg acggcctccc gttcagcacc aatgaaatgc gtgactggca gtcaagttcc
     2101 gtcagacgca tcttgtccta cggcccggaa cgagtcatgg agattggctc gggcaccggg
     2161 atgatgcttt tcagtatcgc accgcactct agtatctact acgctatcga ttcttcgaga
     2221 aaagcagtcg agtttgtgcg ccgtcacctg gggttgttgc cccatgtcac gtgcgagcat
     2281 cgtgaagctc acgcactgcc cgtgatagag caggagttcg acacggtcat cgtcaattct
     2341 gtcatccagt atttccccag tatcgactat ctagtcttgt tcctcgaatg ggcggttcag
     2401 gcagtcggca aaggccgtat atatcttggc gacgtgcgca acctggcgct gctaaacgtc
     2461 ttccatggcg atatattgca ttcccgcata aagggccaaa tccccgccgt tgacttagct
     2521 agccgggccg cagaggcggc aaaagccgat cgtgaattgg tactttcccc tgatttcttc
     2581 gcctctttgg ccaatgtctt cccgcggata tctcatgttg atatcacgtt gcgcgatggt
     2641 gattacgata atgaaatgac cctctatcgc tttgatgtca ccttgcatat tggagagcgt
     2701 gacgagattg gtacgaggcc gagagaggtg gattggcgga ctaaggtcga tttgtccgct
     2761 ctaaaggatg agctaatgga tgctgcgaaa ggcaacaata tgctgcgttt aaacaatgtc
     2821 cctaatggga ggctgcgtaa tgtccacagt cgactcggga ctattctcgg ctacccgtca
     2881 tacgatagct cgtcctgggt ccatccgcgt attctccatg ctgtcgctcg agatgttggt
     2941 atgcagtcgg cacttttgcc cagccggtca aacgatgttt ggagtttcga cgcggtgttt
     3001 tggcgggctg gccagacccc agatctcaca ctgcaaccct ccaaagcaat ggacaagagc
     3061 gcatgggctg aatacgcgaa tgtacctaac atcggaaaag cgatcagtcc ggctaccctc
     3121 gacagagtgc tccgaccatg gctagcagac cggttaccga catatatgat gccatcgttt
     3181 ttcgtggtac tcgacgcctt gccactgaca cccaatggca agatcgaccg caagaggcta
     3241 ccagatccat tagtaagaga cagggcaatt caaaagccag ccacgcaact agaggccgat
     3301 atactggccg tttggtcgcg ggtgctgggc cagaatggta tcgatgtcaa agataacttc
     3361 ttccaggtcg gaggggactc gttacgtgtg actagggtgc aaaccgagtt ggagcaattg
     3421 ctcaggcggc cgatcttgct cgccaaacta ttcgaacatt acaccgtcaa gacactcgcg
     3481 gcatacttag ctgaagatat gagcaatgat gtgaaaacag acccgaaaga ccatgtttct
     3541 gttagccaca atgacgaaga cattgctgta gtatctatgg catgccggct ccctggtgga
     3601 gtcaccaccc ctgaagagtt ttggaagctg cttgaaagcg gaggtgatgc aatcagcgaa
     3661 gtacccaagg ctcggtggga tgcagaggct atctataatg cggatccaga cgcaaaggga
     3721 acatcgtact gtcgtcaagg tgggtttctc gacaccccga ttgactgcta tgatacaggg
     3781 ttttttggaa tttcgccccg ggaagcacgg tcgttggatc caatgcaata catgatgtta
     3841 gagacgtgct gggaatcgtt tgagcgagct gggtatacaa gcgaacagct ccgtggcagc
     3901 cagacaggag tatacgtagg ggtcagtaat attccggcat actcaaaccc taacggcagc
     3961 ggaaaagctc tagaagacct agacggatat gcagggacgg ggtcggctgc cgcgaccatg
     4021 tctgggcgtg tttcgtacac gtttgggctc gaaggtccga gcatgacggt ggacacggcg
     4081 tgctcatctt cgttagtcac gacccatcta gcatgtactg ccttgcgcca aggtgagtgt
     4141 gacatggcag tgtctgctgg ggttagtctg atgctctcgc cgggggtgca tgttgaattt
     4201 agtcgactca ggggaatggc gagagacgga cgatgtcgag ctttcgcggc cgacactgaa
     4261 ggcacaggat ggagcgaggg gtctgtggta atggttctca agcgcctatc tgacgcccag
     4321 cgagatggtg atcgcatcct ggcggtggtg cgtggctcag cagttaatca tggcggacgc
     4381 agcagcgcct ccctcacgac gccgagcgga ccggctcaga aacggctcat tcggactgcg
     4441 ctggccgcgt ctaggttgca ggcgagcgac atcgattaca tagaagctca tgggacaggc
     4501 actaagcttg gtgaccccat cgaaggggtg gcactggcag aggttttcaa ggacggtacc
     4561 cgtgaggagc cgttgtgggt tgggtcagcc aagtccaaca tcggtcatac ccaagcagcc
     4621 gcgggtttgg ctgggatgtt gaaggtggtg ctggccatgc agcacgccat tctaccgcag
     4681 acgttgcatg tatcgaacaa cccgacgcca gcagtggagt gggagagagc aaacatggca
     4741 ctggtgcagg agaagcggtc gtggcggcga cgaaccgcgc ggcgaaaggc aggtatcagc
     4801 gcctttggta tcggtggcac caatgcccat gtaatccttg aggagtggcc gtcgcttgca
     4861 ccagcggtgg ccaatcttga tcctcgttgt gatcctcacg gtaaagatgg tggtattgct
     4921 acacctcctt tgaaaccgct gctgctcctg tcgggccaca gcaatgctgc gctgcgagca
     4981 caggccaaaa aacttttatc ccatttgact aataacagta cggcgcatta ttctctgtac
     5041 gatgtggcct actcactggc aactacacgc aatcatttcc gaagacgaaa ggtgctgatt
     5101 gcgcaggata agagggatct ggcaagccag ctggcaacca ttgctgccgc agagtccact
     5161 aacgcgccag cagaggatat ggtgcagcca tccagaggcc aggtggccat gatattctcc
     5221 ggtcagggta gccaatttct ggatatgggc agagatcttg cacaacactt ccctgttttt
     5281 cgcgacgcct tggaggagat tactgcgcac tttcatggat atttagagcg gccactgttg
     5341 gatgtcatgt ggagtggtga tgctgacagc aggaatgatg ccctgctgca aaggactgac
     5401 tacgcccaac ccgccctgtt ttctttagag gtggcgctgt ggcgtctgtg gaccagctgg
     5461 ggcgtgaggc cagacgtgct gctaggccac agcattggtg aactggcagt agctcacgtc
     5521 gccggcgtgc taaacctgag tgatgctgcc cgtttggttg ctgctcgtgg tcggttgatg
     5581 caggcagtag atactcctgg ggagatggta tcgctagaag ccagtgcttc cgaagtgtct
     5641 gcagccattg acggtttagg gcttggtatg aaggtcgata ttgcctgtca caatgccccc
     5701 tcacaaaccg tcgcctctgg tgatatagat gccgtcaaag ggctacgcac ccactttgcc
     5761 actcggggcc gtaaattcca ttctctaagc gtctcccatg ccttccattc tcaccatatg
     5821 gaggcaatac tggcggatta ccgcgccgta gcagagacag ttgatttcag ccctcctaat
     5881 atacccatcg tcagcagtct aacgggtcag agagtggctc ctggccagct agagcagcct
     5941 gcatactggg tacagcaggc gcgcaacgct gtccgtttca gtgatggaat acaaaccctt
     6001 ctgatggatc acgatatcaa tatcttcttg gaggtagggc cgcaaacggt tttgtgcggc
     6061 atgggtgccg cctgtgtcga tgatattcgc cattccacca ttgcgcctac ctggttgccg
     6121 tcaattattc gggaccggga cgctagctcc gttattcaac agagtcttgc cgaaatacac
     6181 attcaacatg tgtctatcga ttggtcggct tactttaaag ctgccagctg tcaacgtgtc
     6241 gacctgccga cctacgcttt tcaacgggag cctgtgtaca ctataaacag ggacctcaaa
     6301 ttcaagcgta gccccacttc taaccaggtg actagcgtcg caacagctgc tgctcttcat
     6361 ggaacagatc ctctcgatgg gctgcgcagc tccttgatcg tggctgctcc ggcagagcaa
     6421 ctgagaatcg tattatccat gattcgagaa actgtggcca agatcttgga ccatttgtcg
     6481 cccgacgatg tcgacctcga cctccccttg caagacctgg gaattgattc actcactgca
     6541 ttgctgatgc ggaaccagct tacagcgcag actggtttga atttcccggc tatggtgaca
     6601 ctgcatcatc ccaacttaag aagtctgggt cagttcctct tacaccaggc attacaggaa
     6661 acgtcgtcag cttcatcacc tagcagccca aaatcttcta tagcaacgcc tgcttcctca
     6721 ggttcaccac agttgtcacc gacagcggca caagcgatca atggtcattc tgaaccgttg
     6781 cagcacacca aaatgccttc tatcgccccc cttcatcgcg tcttctcccc tctattgggc
     6841 ttgaatcctc caaatacgcc tccacacctg ttctcgtctg ccatgagcta cttcagcaat
     6901 tttgattggt gctcccgcct tatttacgat tcctcgccta cctcatcatc tagtgtgcta
     6961 ccaggttacg gacaggctat tccctttatc ccacaatgct tcaatcccgc tagtccgcgg
     7021 catgatcaat ttatcggcaa ctggctttca aaccacaaga aaacgatcgg tagcgacaat
     7081 gatacaaaac aaagtcctcc tcttcgccac atgctctccc tcttccgacc ccgcgaccct
     7141 tatcacattg aagatccctt gcgccatatc cctcgtgttg cttccctgtt tgctctaggg
     7201 gagggaacgt cgggatacga gggcgttata catgggggct ttacagctac ccttctcgat
     7261 gagagcatca gcatagttca tgagatcaat accgcgctag gcaagacggg caaagtcttc
     7321 gccgccatca atgtcacggc ttctctcagc gtcaagttct ttgctccggt agccgcgacc
     7381 gaagaagtag tatgtgtgac agcgtggata gaaggcatac aagggaacaa tacaacaatg
     7441 aaggcggagc tcacagatct cgcaggaaat aagctagctg aagcagagag cgtgtttgtt
     7501 gccgtgaatc aaaagtgagg agattgataa gacttttcta gttgcatatc ttttatattt
     7561 aaatcttatc tattagttaa ttttttgtaa tttatcctta tatatagtct ggttattcta
     7621 aaatatcatt tcagtatcta aaaattcccc tcttttttca gttatatctt aacaggcgac
     7681 agtccaaatg ttgatttatc ccagtccgat tcatcagggt tgtgaagcat tttgtcaatg
     7741 gtcgaaatca catcagtaat agtgcctctt acttgcctca tagaatttct ttctcttaac
     7801 gtcaccgttt ggtcttttat agtttcgaaa tctatggtga taccaaatgg tgttcccaat
     7861 tcatcgttac gggcgtattt tttaccaatt gaagtattgg aatcgtcaat tttaaagtat
     7921 atctctcttt tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa gccatcgtta
     7981 ttcgatattg tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc gcggtggagc
     8041 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgagact caaacaagat
     8101 atattttgcc cgccttacag acgaaactag ttggaggtta tggagcatac tatcacgtgg
     8161 gcggccactg gtgagttact acaccccagg ggcaacgttg atgctcctaa aaaactctgg
     8221 ctggacgcaa gccgtaacac ccgtgtcact tcataatcgt ttgcaattca gggcttgatc
     8281 tacacattac cctgttatcc ctattaatta atacggttat ccacagaatc aggggataac
     8341 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8401 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8581 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8641 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8701 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8761 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8821 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8881 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8941 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9001 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9061 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10141 tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg
    10201 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
    10261 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
    10321 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
    10381 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
    10441 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
    10501 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
    10561 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
    10621 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
    10681 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
    10741 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
    10801 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
    10861 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
    10921 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
    10981 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
    11041 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
    11101 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
    11161 gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc
    11221 gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat
    11281 acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt
    11341 tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta
    11401 tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg
    11461 attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat
    11521 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    11581 tttcggtgat gacggtgagc gcgtttcggt gatgacggtg aaaacctcgg cgcgccatta
    11641 ccctgttatc cctaatatac aacgcaaaga agctggaaaa ttggcgaggg aatcctgttt
    11701 ctgtctatcc aagaatgggc atgaggtggc aaccgtcgtg ctagcgtaca gggtgcactt
    11761 tgtaaccatt tgggacaccg gacactcgct gttttcgaaa ttacccttta agcgcgggta
    11821 ttgaaccagg cttatgccca agatcgtagc aagcatttaa ctgtgggaat actcaggtat
    11881 cgtaagatgc aagagttcga atctcttagc aaccattatt tttttcctca acataacgag
    11941 aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt
    12001 tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg
    12061 tgagagcgcc ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg
    12121 catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg
    12181 gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat
    12241 atatatttca aggatatacc attctaatgt ctgcccctaa gaagatcgtc gttttgccag
    12301 gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg
    12361 atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg
    12421 atgctacagg tgttccactt ccagatgagg cgctggaagc ctccaagaag gctgatgccg
    12481 ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag
    12541 gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg
    12601 catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact
    12661 tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg
    12721 gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa
    12781 gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata
    12841 aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga
    12901 acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag
    12961 ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca
    13021 tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg
    13081 cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc
    13141 cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct gcaatgatgt
    13201 tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg
    13261 ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg
    13321 gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat
    13381 gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa
    13441 atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga
    13501 aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa tggctcaacg
    13561 aaatgacggt atcgataagc ttgatatcga attcctgcag cccgggggat ccactagttc
    13621 taga
//
