LOCUS       sequence-210705-       12004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU65.1-2.2
FEATURES             Location/Qualifiers
     source          1..12004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             511..534
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6435..6455)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6435..6453)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6792..7380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6872..6891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7551..8342,8343..8411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8174..8193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8412..8516)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8543..9885
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9506..9553)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9926..9944
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10239..10646
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10647..11741
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11988..12004)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11988..12004)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttaattaata tctaaaaatt gccttatgat ccgtctctcc ggttacagcc tgtgtaactg
       61 attaatcctg cctttctaat caccattcta atgttttaat taagggattt tgtcttcatt
      121 aacggctttc gctcataaaa atgttatgac gttttgcccg caggcgggaa accatccact
      181 tcacgagact gatctcctct gccggaacac cgggcatctc caacttataa gttggagaaa
      241 taagagaatt tcagattgag agaatgaaaa aaaaaaaaaa aaaaaggcag aggagagcat
      301 agaaatgggg ttcacttttt ggtaaagcta tagcatgcct atcacatata aatagagtgc
      361 cagtagcgac ttttttcaca ctcgaaatac tcttactact gctctcttgt tgtttttatc
      421 acttcttgtt tcttcttggt aaatagaata tcaagctaca aaaagcatac aatcaactat
      481 caactattaa ctatatcgta atacacaatg gattataagg atgatgatga taagactagt
      541 atgaacgcca tctatttcag caacgaattc cccaaggagg atttgaagga tgtcttccga
      601 cagctgcaca aggagagcaa gagcagcatc caccatctgc tcgcacgctt tatgagtgag
      661 gccacgaggg ctgtgaagaa ggagattgag cagctaccgt ccagcctgaa gcagataata
      721 cctccttttg agactctcag tgcttgggcg gagcagaagg aactccgcga aggtgaactg
      781 tgtggcgcta tcgatggcgt tttactagtt ctacttcaga tatcagtata catatgccat
      841 gctgaggccg gtgcagagct cccaactgct ccgcacctat ctgcgcttgg aattggactc
      901 ctcggagccg ccgctgtttc catgtccaag cagatctctg acctgccctc ctacggtgct
      961 caagctatcg ctattgcttt ccggttgggt gttcacgttc agggtgtgtc tcaccagctt
     1021 gaggcccgcg agctcgacga gactcccaag acttgggctt atgttgttca caacatcgac
     1081 cccaaggatg ctcaggctga gctggaggca ttccacgagg cgaacttgac atcaaacact
     1141 gcccgcatat ttatcagcgc cgttagccgt acctccgtta cgatcagtgg cccgccctcg
     1201 aagctcgaga gcctcttcaa gaagtccaag gttttccggg atgcacgatc aatcgccctg
     1261 cccgtctatg gcggcctttg ccatgctcct agtgtctaca gcacaaagga cacacaagcc
     1321 atcatcgacg gcttgaacac gccgacatct gcagctttgt ccagccaagc tccccaagca
     1381 ccgctgtact ctacgagcac cggaaagcca tatgaggcag tcaacgccat cgggctgttc
     1441 tcatgcgtcg tgaccgaact attgaccaaa gccatcgagt gggatcaagt cattgccggt
     1501 ctagtcagtg ctgctaagtc ggctgaagca actgaggtta cgctctcctg cttcggcaac
     1561 tcgattccac tgaacgacct caagacagcg cttcaagctg ccatcgccga tgtcaagatc
     1621 accattcaca gcttcatgac cgttctcaag aagaacgcaa ctggcggtgc tcctccccgc
     1681 ggtacagccc agtcgaagct tgccattgtt ggaatgtcat gcaggcttcc tggtggcgcc
     1741 accgacactg agaagttctg ggagcttctt gaagccggtc tagatgtctc tcgcaagatt
     1801 ccggcttctc gctttgacat tgaaacccat tacgatccag agggtaagga cttgaacaag
     1861 accatgactc aatacggttg ctttatcgat gagcccggca tgttcgatcc cagcttcttc
     1921 aacatgtctc cccgtgaagc catggctgtt gatccccaga tgcgcctgtc tctcgtcact
     1981 gcctatgaag ctttggagaa agctggcttt gttggcaacc gcactctttc caccaagctc
     2041 cagcgcatcg gaacattcta cggacaggca gctgacgatt accgtgaggt gaaccagggt
     2101 caagaggttg gcacttacta catccctggt ggatgccgtg ccttcggacc tggtcgcatc
     2161 aactacttct tcaagtttgc cggacctagc tacagcgttg atactgcttg ctcttctggg
     2221 cttgcagcta tcgaggttgc ttgccaggct ctgtggaacg gcgaggttga catggctgtc
     2281 gctggtggtg tcaacatcct aacaaacccc gatggtttcg ctggactctg caggggtcac
     2341 ttcctcacca agggccagaa cgcttgcaag acttgggatg ccaccgctga tggctactgc
     2401 cgtgctgagg gtgtgggatc tattgtcatc aagaggctcg aagacgcaga ggcggacaac
     2461 gacaacattc ttggtgtcgt cctcgctgcc ggaaccaacc actctgccga agctgtctcc
     2521 attactcacc cacacgctgg acaccaatct tatctgtccc gtcagatcct ccgacaggca
     2581 ggagttgatc ctttggatgt ctcctacatt gagcttcacg gcactggcac ccaggcaggc
     2641 gatttcgagg agctgaaggg tgttctggac gtctatgcac caaacacctc ccctagtcgt
     2701 cgcactgacc aggttctcca cattggaagc tccaagtcca acgttggcca cggcgagtcg
     2761 gtcgcaggta cgaccgcact gatcaaggtt cttttgatgc ttcagaagaa cgccatcccc
     2821 cgccacattg gtatcaagac tgagatcaac ccgcgtcttc ccactgatct tgatcagagg
     2881 aacgtcgtca ttcccttttc caacacccca tgggctcctg gaaagcgcat tgccgctgtc
     2941 aacaactttg gagcagctgg tggcaacacc atgatgatct tggaagaggc ccctgcccgc
     3001 gaagcacgtc aagagaaaga cccccgtcag actcatgttg tgaccctctc agccaagacc
     3061 aaggcctcgc tccgtggcaa catcgaacgc ctcctcgctc atctcgagag ggacgaaacc
     3121 gtggcattgg aggatcttgc ctacaccacc actgcacgca agtaccacca ctcataccgg
     3181 atagccatct ccgcttccga tattggttcc gtcaagaagc agcttacttc gcagctgaac
     3241 aagattgatg ctttgaagcc tgtcaacaag accaaccccc cgtctgtggc tttcgccttc
     3301 accggtcagg gcgcatctta ccagtcgatg aacctcgagc tctaccgcga tgtccccgtc
     3361 ttccgagagc acatccgcac ccttgacaag ctggctcagg ctcaaggctt cccgtccttc
     3421 attcccgcta tcgacggctc gcaccccaag gaatatgttc atgctcctgt aatcacccag
     3481 ctagcactcg tctgtgtcga gattgcgctc gcgcactact gggcttccct cggcatcaag
     3541 cctgacgttg ttgttggcca ctctctcggc gagtacgcag ctatgcacgt cgctggtgtc
     3601 atcagcgcca acgacgccat cttcatggtc ggttccaggg ccctgatgct catggagaag
     3661 tgccagtcag gcagccactg catgatggct gtacgcgcct ctctggaaaa catcatcgcc
     3721 aactcaggcg gcaagcccca cactgtggcc tgcatcaacg gcccagctga tactgtcttg
     3781 agcggcacaa aggcccagat ggacgacatc gccaaggacc tcgaggctgc tggactccga
     3841 tgcatcaagc ttgatgttgc ctttgccttc cactccgagc agactgaccc cattctcgac
     3901 gaatttgagg ccatcagcaa gagcggtgtc gtgttccatg agcccaagct gcccatcatc
     3961 tcgccccttc tcggcaaggt catcttcgat ggccacactt tgaacgccaa ctatgtgcgc
     4021 accgccaccc gcgaggctgt cgactttgtc tctgccatga ggaacgcttc cagcatttcc
     4081 accatcagcg acgacaccgt ctggattgag attggtcccc accccgtctg cgttggattc
     4141 atcaagacca ccctgccttc tacccgcctt gcagttcctt ctctgcgccg tggagacagc
     4201 aactggaaga ccatgtcgga tagtgttgca gctcttcacc ttgctggtgt caatgtcgac
     4261 tggaacgagc tccaccgtcc tttctctggc caggtccgcc ttcttgagct tccgacctat
     4321 gcttgggacg agaagaacta ctggatgcag tacaacggtg actggtgcct gaccaagggc
     4381 aacactttct acgagaagaa gcagaagacc atcgagcccg ccatcggcaa caccaagcag
     4441 taccagtcat tgggctcttt ggttcagaac attatctcag ttaatgtcga gggcgaggcg
     4501 ggtagtgtgg tcatgcagtc cgacctcatg cagaaggagt tcttggaggc agcttcagga
     4561 catcgtatga acgacaatgc tgtcgtcaca tcgtctattc atgcggatat cgcttacacc
     4621 ctgggtgact acctctaccg caagttttac cccaaggcca agcgcccgga tatggacatg
     4681 gccaaccttg aggtcaccaa ggctctcatt gctaagaaga aaggcatgga tacacctcag
     4741 tctatccaag tcactgccag caccaccaat atccgatctg gtgtcctgga cctgacttgg
     4801 cagaacgtag aatctgatgg cagctgcgct gagccttttg ctacagccca gatcatctat
     4861 ggcgatgcta agcagtggct ctcatcctgg agccccatgt cccatcttat ccaaagccgc
     4921 atcgagtcac ttgagcgtct agctgtcgag ggacaagcta accgtctatc ccgcaacttg
     4981 gcctacactc tattctcctc caacctagtc gactatgccg acaagtaccg cggcatgcaa
     5041 tccgtcgtca tgaacgacct agaagccttt gccgaagtta agcttaccga gaagcccgac
     5101 agcggcacct acgccgtgcc accctactac atcgacagtg tagcgcattt ggcgggattc
     5161 atcatgaact gctccgactc tattgacacc aagaacaact attgcgttac ttctggctgg
     5221 aagtctatgc gcttcgccga gcccatgact ccgggagcta gataccgatc ttatgtcaag
     5281 atgattccta ccaaggagga cccagctgtc ttccttggtg atgtttacat tctcaaggca
     5341 gaagacaact ctattgttgg aatggttggt ggcatccact tccgccgctt ccctcgtatc
     5401 ctgctcaacc gcttcttcag cgcccccgag cctgctaaag cgtcgtccgg tcagcaagcc
     5461 aagccagcac ccgcaaaggc cgcgcctgca cccaagcccg tcgcacaaaa gaccatcgct
     5521 gctgccccca aggcacagga ctttgacgct caatctagca gcagctcgga ggtgactcct
     5581 cctggcaccc cgctgcttgc tgcctcggcc aacacttctt ccactagcat caacgtcatt
     5641 gctgccgagc cagcgaagaa gggcggcaac ggtatcatgg acaaagcgct tgacatcatc
     5701 gccaacgaga cagctattga cctggccgac ctggaagatg acgccgactt tgccaatctt
     5761 ggtattgata gtttgatgtc gcttgtgcta gtagagaagt tccagaagga gcttgatgtc
     5821 aaggttaacg gcagtttgtt tttggattat cctaccattg gcgatttgcg cgagtggttg
     5881 gagaagtact atggttaggg agattgataa gacttttcta gttgcatatc ttttatattt
     5941 aaatcttatc tattagttaa ttttttgtaa tttatcctta tatatagtct ggttattcta
     6001 aaatatcatt tcagtatcta aaaattcccc tcttttttca gttatatctt aacaggcgac
     6061 agtccaaatg ttgatttatc ccagtccgat tcatcagggt tgtgaagcat tttgtcaatg
     6121 gtcgaaatca catcagtaat agtgcctctt acttgcctca tagaatttct ttctcttaac
     6181 gtcaccgttt ggtcttttat agtttcgaaa tctatggtga taccaaatgg tgttcccaat
     6241 tcatcgttac gggcgtattt tttaccaatt gaagtattgg aatcgtcaat tttaaagtat
     6301 atctctcttt tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa gccatcgtta
     6361 ttcgatattg tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc gcggtggagc
     6421 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgagact caaacaagat
     6481 atattttgcc cgccttacag acgaaactag ttggaggtta tggagcatac tatcacgtgg
     6541 gcggccactg gtgagttact acaccccagg ggcaacgttg atgctcctaa aaaactctgg
     6601 ctggacgcaa gccgtaacac ccgtgtcact tcataatcgt ttgcaattca gggcttgatc
     6661 tacacattac cctgttatcc ctattaatta atacggttat ccacagaatc aggggataac
     6721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7201 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7681 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7861 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8161 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8521 tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg
     8581 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     8641 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
     8701 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     8761 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     8821 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     8881 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     8941 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     9001 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     9061 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     9121 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     9181 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     9241 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     9301 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     9361 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     9421 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
     9481 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
     9541 gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc
     9601 gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat
     9661 acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt
     9721 tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta
     9781 tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg
     9841 attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat
     9901 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     9961 tttcggtgat gacggtgagc gcgtttcggt gatgacggtg aaaacctcgg cgcgccatta
    10021 ccctgttatc cctaatatac aacgcaaaga agctggaaaa ttggcgaggg aatcctgttt
    10081 ctgtctatcc aagaatgggc atgaggtggc aaccgtcgtg ctagcgtaca gggtgcactt
    10141 tgtaaccatt tgggacaccg gacactcgct gttttcgaaa ttacccttta agcgcgggta
    10201 ttgaaccagg cttatgccca agatcgtagc aagcatttaa ctgtgggaat actcaggtat
    10261 cgtaagatgc aagagttcga atctcttagc aaccattatt tttttcctca acataacgag
    10321 aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt
    10381 tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg
    10441 tgagagcgcc ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg
    10501 catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg
    10561 gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat
    10621 atatatttca aggatatacc attctaatgt ctgcccctaa gaagatcgtc gttttgccag
    10681 gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg
    10741 atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg
    10801 atgctacagg tgttccactt ccagatgagg cgctggaagc ctccaagaag gctgatgccg
    10861 ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag
    10921 gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg
    10981 catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact
    11041 tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg
    11101 gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa
    11161 gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata
    11221 aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga
    11281 acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag
    11341 ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca
    11401 tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg
    11461 cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc
    11521 cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct gcaatgatgt
    11581 tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg
    11641 ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg
    11701 gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat
    11761 gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa
    11821 atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga
    11881 aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa tggctcaacg
    11941 aaatgacggt atcgataagc ttgatatcga attcctgcag cccgggggat ccactagttc
    12001 taga
//
