LOCUS       sequence-210707-        7044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose inducible VioB-mCherry expression (weaker RBS). Used to 
            test burden response in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, 
            Awan AR, Gilbert C, Stan GB, Ellis T
  TITLE     Burden-driven feedback control of gene expression.
  JOURNAL   Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635.
  PUBMED    29578536
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635."
COMMENT     Sequence Label: pSB1C3-H3
FEATURES             Location/Qualifiers
     source          1..7044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      10..67
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(91..108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(262..850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(342..361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1033..1127)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1148..1807)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1722..1741
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1969..1987
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1991..2034)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2037..2058
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(2059..2937)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2964..3248
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3171..3190
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(6488..6506)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6756..6775
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(7033..7044,1..9)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
       61 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
      121 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      181 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      241 aaaggccgcg ttgctggcct ttttccacag gctccgcccc cctgacgagc atcacaaaaa
      301 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      361 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      421 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      481 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      601 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      661 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      721 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      781 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      841 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      901 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      961 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1021 ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1081 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1141 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1201 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1261 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1321 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1381 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1441 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1501 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1561 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     1621 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1681 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1741 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1801 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     1861 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg
     1921 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1981 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
     2041 tcgcggccgc ttctagagtt atgacaactt gacggctaca tcattcactt tttcttcaca
     2101 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata
     2161 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct
     2221 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc
     2281 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
     2341 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
     2401 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa
     2461 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
     2521 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
     2581 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
     2641 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
     2701 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg
     2761 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
     2821 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac
     2881 cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact
     2941 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg
     3001 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag
     3061 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
     3121 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
     3181 atttttatcc ataagattag cggatcctac ctgacacttt ttatcgcaac tctctataat
     3241 ttctccatac ccgttttttt gggctagcat tacccgcatc acaaggaaaa gcccaatatg
     3301 agcattctgg atttcccgcg tatccacttc cgtggctggg cccgtgtcaa tgcgccgacc
     3361 gcgaaccgcg atccgcacgg ccacatcgat atggccagca ataccgtggc gatggcgggt
     3421 gagccgttcg acctggcacg ccatcctacg gagttccacc gtcacctgcg ctccctgggt
     3481 ccgcgcttcg gcttggatgg tcgtgctgac ccggaaggcc cgttcagcct ggccgagggc
     3541 tacaacgctg ccggtaacaa ccacttttcg tgggagagcg caaccgttag ccacgtgcaa
     3601 tgggatggcg gtgaggcgga tcgtggtgac ggtctggtcg gtgctcgttt ggcactgtgg
     3661 ggtcactaca atgattatct gcgtaccacc ttcaatcgtg ctcgttgggt cgacagcgac
     3721 ccgacgcgcc gtgacgctgc acaaatctat gcgggccaat tcaccattag cccggctggt
     3781 gccggtccgg gtacgccgtg gctgtttacg gcagacattg atgatagcca tggtgcacgt
     3841 tggacgcgtg gcggccacat tgcagagcgt ggcggccact tcttggatga agagtttggt
     3901 ctggcacgcc tgtttcagtt ctctgtgccg aaagatcacc cacattttct gtttcacccg
     3961 ggtccgtttg attccgaggc ctggcgtcgt ctgcaattgg ctctggagga tgacgacgtt
     4021 ctgggtctga ccgtgcaata tgcgttgttc aatatgagca ccccgcctca gccgaacagc
     4081 ccggtttttc acgatatggt cggtgttgtc ggtctgtggc gtcgtggtga actggcgagc
     4141 tacccggctg gtcgtctgct gcgtccgcgt caaccgggtc tgggtgacct gaccctgcgc
     4201 gtcaacggtg gtcgcgttgc gctgaatttg gcgtgtgcca ttccgttcag cactcgtgcc
     4261 gcgcagccaa gcgcaccgga ccgcctgacc ccggacctgg gtgccaaact gccgctgggc
     4321 gatctgctgc tgcgtgatga ggacggcgca ctgttggcac gtgtgccgca ggctctgtac
     4381 caagactatt ggacgaatca cggtattgtg gacctgccgc tgctgcgcga accgcgtggt
     4441 agcttgaccc tgagcagcga actggcggag tggcgtgagc aagactgggt cacccaaagc
     4501 gacgcgtcta acctgtacct ggaggcaccg gatcgccgtc acggtcgctt tttccctgag
     4561 agcatcgcgc tgcgcagcta ctttcgcggt gaagcgcgtg cgcgtccgga tatcccgcat
     4621 cgtatcgagg gcatgggcct ggtcggcgtc gaatctcgtc aggatggcga cgctgcggaa
     4681 tggcgtctga cgggtctgcg tccgggtccg gcacgcattg ttctggacga tggtgccgag
     4741 gcgatccctc tgcgtgttct gcctgacgat tgggcgctgg atgacgcgac cgtcgaagaa
     4801 gtggattacg cctttttgta ccgccacgtt atggcgtatt acgagctggt gtatccattc
     4861 atgagcgaca aggtgttttc cctggctgat cgttgcaaat gtgaaacgta cgcacgtctg
     4921 atgtggcaga tgtgtgatcc gcagaaccgc aacaagtcct attacatgcc gagcacccgc
     4981 gaactgtcgg caccgaaagc tcgtttgttc ttgaagtatc tggcccacgt ggaaggccag
     5041 gcacgcctgc aagcacctcc gccagcgggt ccggcacgca ttgaatctaa agcccagttg
     5101 gcggcagagc tgcgtaaagc cgtcgacctg gagctgtctg tgatgctgca atacctgtac
     5161 gcggcgtata gcattccgaa ctatgcacag ggccaacaac gtgttcgtga cggtgcgtgg
     5221 accgccgagc agctgcaact ggcgtgcggt agcggtgacc gtcgccgtga tggcggtatt
     5281 cgtgcagcac tgctggaaat tgctcatgaa gaaatgattc attacctggt cgttaacaac
     5341 ctgctgatgg ccctgggcga gccgttctac gcgggtgtcc cgctgatggg cgaagcggca
     5401 cgtcaggcgt ttggcctgga caccgagttc gctctggaac cgtttagcga aagcacgctg
     5461 gcacgttttg ttcgtctgga atggccgcac tttatcccag caccgggcaa atccatcgcg
     5521 gactgctatg ccgccattcg tcaggcgttt ttggatctgc cggacttgtt tggtggcgag
     5581 gcaggtaagc gtggcggtga acaccacctg ttcctgaatg agctgaccaa ccgtgcgcat
     5641 ccgggttatc aactggaagt tttcgatcgc gactcggcgc tgtttggtat tgcatttgtg
     5701 accgatcagg gcgaaggtgg cgctctggac agcccgcact acgaacatag ccattttcaa
     5761 cgtctgcgtg aaatgagcgc gcgtatcatg gctcaaagcg caccgttcga accggcgctg
     5821 ccggcgttgc gtaatccggt tctggatgag agcccgggtt gccaacgtgt cgcagacggt
     5881 cgtgcgcgtg cgctgatggc attgtaccaa ggcgtttatg agctgatgtt tgcgatgatg
     5941 gcgcagcact tcgccgtgaa accgctgggt agcttgcgtc gcagccgcct gatgaacgca
     6001 gcaatcgatc tgatgaccgg tctgttgcgt ccgctgagct gcgcgctgat gaacctgcca
     6061 agcggcatcg ccggtcgcac ggccggtccg ccgctgccgg gtccggttga cacccgtagc
     6121 tatgacgact acgcgctggg ctgtcgcatg ctggcacgcc gttgcgagcg tctgctggag
     6181 caggcgagca tgctggaacc gggttggctg ccggatgcgc agatggagct gctggatttc
     6241 tatcgtcgcc aaatgctgga cttggcgtgc ggcaaactga gccgcgaggc cgccgaagct
     6301 gcagcaaagg aggccgcagc taaagaggct gcagcgaaag cgatggtgag caagggcgag
     6361 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     6421 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     6481 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     6541 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     6601 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     6661 ggcggcgtgg tgaccgtgac ccaggactcc tccttgcagg acggcgagtt catctacaag
     6721 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     6781 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     6841 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     6901 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     6961 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     7021 tccacctaat aatactagta gcgg
//
