LOCUS       sequence-210711-        9706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose inducible expression of the Vibrio Fischery Lux operon. 
            Used to test burden response in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, 
            Awan AR, Gilbert C, Stan GB, Ellis T
  TITLE     Burden-driven feedback control of gene expression.
  JOURNAL   Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635.
  PUBMED    29578536
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635."
COMMENT     Sequence Label: pSB1C3-Lux
FEATURES             Location/Qualifiers
     source          1..9706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      4..61
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(85..102)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(256..844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(336..355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1027..1121)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1142..1801)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1716..1735
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1963..1981
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1985..2028)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2031..2052
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(2053..2931)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2958..3242
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3165..3184
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    join(9689..9706,1..3)
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
       61 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      121 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      181 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      241 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
      301 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      361 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      421 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      481 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      541 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      601 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      661 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      721 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      781 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      841 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      901 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      961 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     1021 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1081 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1141 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1201 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1261 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1321 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     1381 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1441 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1501 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1561 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     1621 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1681 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1741 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1801 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1861 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct
     1921 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     1981 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     2041 ccgcttctag agttatgaca acttgacggc tacatcattc actttttctt cacaaccggc
     2101 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
     2161 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
     2221 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
     2281 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
     2341 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
     2401 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
     2461 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
     2521 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc
     2581 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
     2641 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
     2701 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt
     2761 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
     2821 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
     2881 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
     2941 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg
     3001 cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct
     3061 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg
     3121 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt
     3181 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
     3241 ataccgtttt tttgggctag cagggaaaat aatgaataaa tgtattccaa tgataattaa
     3301 tggaatgatt caagattttg ataattatgc atataaagaa gtaaaactaa ataatgataa
     3361 tagagtaaaa ttatctgtca ttactgaaag ttcagtctca aaaacattaa atatcaaaga
     3421 tagaattaat ctaaatttaa atcagattgt gaatttttta tataccgttg gtcaacgatg
     3481 gaaaagtgaa gaatataatc ggcgacgaac ctatatccgt gagttaaaaa catatcttgg
     3541 ttattctgat gaaatggcaa gattagaagc gaattggatt gcaatgttat tgtgctctaa
     3601 aagtgctttg tatgacattg ttaattatga tttgggctct atacacgtat tagatgaatg
     3661 gcttccacgt ggtgattgct atgttaaagc acaaccgaaa ggtgtttctg ttcacttgtt
     3721 agctggtaat gttccattat caggagtgac atctattttg cgtgctattt taacaaaaaa
     3781 tgagtgcatt attaaaacct cgtcttcaga tccttttact gcaaacgctt tagtttccag
     3841 ttttattgat gttaatgcag accatccaat aaccaaatca atgtctgtta tgtattggcc
     3901 gcatgatgaa gatatgactc tatctcaaag aataatgaat catgccgacg tggttattgc
     3961 ttggggtgga gacgaggcga ttaaatgggc ggtaaaatat tcaccaccgc atgtcgatat
     4021 tctgaaattt ggaccaaaga aaagcttaag tattattgaa gctcctaaag atatagaagc
     4081 cgcagcaatg ggggttgctc atgatatttg tttctatgac cagcaagcct gcttctctac
     4141 tcaagacgtt tattatatag gagataattt acctttattt ttaaatgaac ttgaaaaaca
     4201 gctagatcga tacgcgaaaa ttttaccaaa aggttcaaat agttttgatg aaaaagcggc
     4261 gtttactctt actgaaaaag aaagtctatt tgctggatat gaagtgagaa agggagataa
     4321 gcaagcttgg ttaatagtcg tatcacctac aaatagcttt ggaaatcaac cgctatcacg
     4381 aagtgtgtat gttcatcaag tatctgatat taaagagata attccttttg ttaataaaaa
     4441 tagaacacaa actgtttcta tttatccttg ggaagcgtca ttaaaatatc gagataaatt
     4501 agcaagaagt ggagttgaaa gaattgttga atcaggcatg aataatattt tcagagttgg
     4561 aggggctcat gattcattat ctcctctcca gtacctagtt aggtttgtat cgcatgagag
     4621 accatttaat tatacgacaa aagatgttgc ggttgaaatc gaacaaacac gttacttaga
     4681 ggaagataag tttttagttt ttgtcccata gttaaaggaa attatatgaa agatgaaagt
     4741 gcttttttta cgattgatca catcatcaag cttgataatg gtcagtctat ccgagtttgg
     4801 gaaacactcc ctaaaaagaa cgtaccagag aaaaaacata caatacttat tgcttcgggt
     4861 tttgctagaa gaatggatca ttttgcaggt cttgctgagt atttatctac taacggtttt
     4921 catgtcattc gctacgattc tttgcatcat gttggattaa gcagtggatg tataaatgaa
     4981 tttacgatgt cgattggaaa aaatagcctg cttacagtcg tagattggct tacagatcat
     5041 ggtgtcgaac gaatagggct gattgctgct agtttgtcag cgagaatcgc ctatgaggta
     5101 gtaaataaaa ttaaattatc atttttaatt acggccgtag gtgtcgttaa tcttagagat
     5161 acattagaaa aagcattgga gtatgactat ttgcaattac ctatttcaga gttaccagaa
     5221 gatcttgact tcgaaggtca taatttagga tcggaggtct ttgttacaga ttgctttaaa
     5281 cataattggg acacgttaga ctcgacactt aatagtgtta aaggattagc gattccattt
     5341 attgctttta ctgcaaacga tgatagttgg gtaaagcaaa gtgaagttat agagctcatt
     5401 gatagcattg aatctagtaa ttgtaagctc tattcgctaa ttggaagttc acatgatctt
     5461 ggggaaaatt tggttgtatt aagaaatttt tatcaatcag tgacgaaggc agccttagca
     5521 ttagatgatg gtttattgga tttagagata gacattattg aacctcgatt tgaggacgtt
     5581 acaagtatta ctgttaagga gcgtagatta aaaaatgaaa ttgaaaatga attattagaa
     5641 ttggcttaaa taaacagaat caccaaaaag gaatagagta tgaagtttgg aaatatttgt
     5701 ttttcgtatc aaccaccagg tgaaactcat aagcaagtaa tggatcgctt tgttcggctt
     5761 ggtatcgcct cagaagaggt agggtttgat acatattgga ccttagaaca tcattttaca
     5821 gagtttggtc ttacgggaaa tttatttgtt gctgcggcta acctgttagg aagaactaaa
     5881 acattaaatg ttggcactat gggggttgtt attccgacag cacacccagt tcgacagtta
     5941 gaagacgttt tattattaga tcaaatgtcg aaaggtcgtt ttaattttgg aaccgttcga
     6001 gggctatacc ataaagattt tcgagtattt ggtgttgata tggaagagtc tcgagcaatt
     6061 actcaaaatt tctaccagat gataatggaa agcttacaga caggaaccat tagctctgat
     6121 agtgattaca ttcaatttcc taaggttgat gtatatccca aagtgtactc aaaaaatgta
     6181 ccaacctgta tgactgctga gtccgcaagt acgacagaat ggctagcaat acaagggcta
     6241 ccaatggttc ttagttggat tattggtact aatgaaaaaa aagcacagat ggaactctat
     6301 aatgaaattg cgacagaata tggtcatgat atatctaaaa tagatcattg tatgacttat
     6361 atttgttctg ttgatgatga tgcacaaaag gcgcaagatg tttgtcggga gtttctgaaa
     6421 aattggtatg actcatatgt aaatgcgacc aatatcttta atgatagcaa tcaaactcgt
     6481 ggttatgatt atcataaagg tcaatggcgt gattttgttt tacaaggaca tacaaacacc
     6541 aatcgacgtg ttgattatag caatggtatt aaccctgtag gcactcctga gcagtgtatt
     6601 gaaatcattc aacgtgatat tgatgcaacg ggtattacaa acattacatg cggatttgaa
     6661 gctaatggaa ctgaagatga aataattgct tccatgcgac gctttatgac acaagtcgct
     6721 cctttcttaa aagaacctaa ataaattact tatttgatac tagagataat aaggaacaag
     6781 ttatgaaatt tggattattt tttctaaact ttcagaaaga tggaataaca tctgaagaaa
     6841 cgttggataa tatggtaaag actgtcacgt taattgattc aactaaatat cattttaata
     6901 ctgcctttgt taatgaacat cacttttcaa aaaatggtat tgttggagca cctattaccg
     6961 cagctggttt tttattaggg ttaacaaata aattacatat tggttcatta aatcaagtaa
     7021 ttactaccca tcaccctgta cgtgtagcag aagaagccag tttattagat caaatgtcag
     7081 agggacgctt cattcttggt tttagtgact gcgaaagtga tttcgaaatg gaatttttta
     7141 gacgtcatat ctcatcaagg caacaacaat ttgaagcatg ctatgaaata attaatgacg
     7201 cattaactac aggttattgt catccccaaa acgactttta tgattttcca aaggtttcaa
     7261 ttaatccaca ctgttacagt gagaatggac ctaagcaata tgtatccgct acatcaaaag
     7321 aagtcgtcat gtgggcagcg aaaaaggcac tgcctttaac atttaagtgg gaggataatt
     7381 tagaaaccaa agaacgctat gcaattctat ataataaaac agcacaacaa tatggtattg
     7441 atatttcgga tgttgatcat caattaactg taattgcgaa cttaaatgct gatagaagta
     7501 cggctcaaga agaagtgaga gaatacttaa aagattatat cactgaaact taccctcaaa
     7561 tggacagaga tgaaaaaatt aactgcatta ttgaagagaa tgcagttggg tctcatgatg
     7621 actattatga atcgacaaaa ttagcagtgg aaaaaacagg gtctaaaaat attttattat
     7681 cctttgaatc aatgtccgat attaaagatg taaaagatat tattgatatg ttgaaccaaa
     7741 aaatcgaaat gaatttacca taataaaatt aaaggcaatt tctatattag attgcctttt
     7801 taaatttctg ttgatattag gtattactgg agagggtatg actgtccata ctgaatataa
     7861 aagaaatcaa atcattgcta gttcagaaat tgatgatctt atctttatga cgaaaccaca
     7921 agagtggtca tttgaagagc aaaaagaaat acgggataaa ttagttcgtg aggcttttta
     7981 ttttcactac aatagaaatg aagaatatag aaattattgt atcaatcagc atgtgagtga
     8041 taatttacac actattgatg aaatacccgt gtttccaaca tctgttttta aatataagaa
     8101 attacatact gtcacagccg aggacattga aaattggtat acaagtagtg gaactcgtgg
     8161 agtaaaaagt catattgcac gtgatcgtct tagcattgaa cgcttgcttg gttctgtcaa
     8221 cttcggaatg aaatacgttg gagattggtt tgagcatcaa atggaattga taaatttagg
     8281 accagataga ttcaatacaa ataatatttg gtttaaatat gtcatgagtt tggtcgagtt
     8341 actttatccg actgaattta cagttgataa tgacaaaata gattttgaaa aaacagtaaa
     8401 acatctattt agaattaaga atagtaaaaa agacatttgc ttaattgggc caccattttt
     8461 tgtgtatctt ttgtgccaat atatgaaaga aaacaatatt gaatttaaag gaggagatag
     8521 agtacatatt attactggtg gaggatggaa atctaatcag aatgactctt tagatcgtgc
     8581 tgattttaat caattattaa tggatacttt ccaactcgac aagattaatc aaattagaga
     8641 tacctttaat caagttgagc ttaatacttg tttttttgaa gatgaatttc aaagaaaaca
     8701 tgttccaccg tgggtatatg ctcgggctct tgatcctgaa accttgaaac ccgtagcaga
     8761 tggtgagatc gggttgttaa gttatatgga cgcctcatca actgcttacc ctgcttttat
     8821 tgttactgat gatatcggta ttgtaaaaga aattagagaa ccagatcctt acccaggggt
     8881 aactgttgag attgttcggc gcttaaatac acgtgcgcaa aaaggatgcg cgctctctat
     8941 ggctaatgtc atacaaaaga atatcaagga ttaagttatg attgttgatg gtagagtttc
     9001 aaaaatagta ttagcgtcaa taagaaataa tatatataag gtatttatta ctgtaaattc
     9061 accaataaag ttcatcgctg gacaatttgt aatggtcacg attaatggga aaaaatgccc
     9121 tttttcaatt gcgaattgcc cgacaaaaaa ttacgaaata gaattgcata ttggtagttc
     9181 gaatagagac tgctcattgg atattatcga atattttgtc gatgctcttg ttgaggaagt
     9241 cgcaattgag ttagatgctc cccatggaaa cgcttggtta cggtctgaaa gtaataaccc
     9301 attgctatta attgcgggag gtacaggttt atcatatata aatagcattc taacaaattg
     9361 cttaaatagg aatatacctc aagatattta tctttactgg ggagtaaaag acagttctct
     9421 tttgtatgaa gatgaagagt tactaaactt atcactaaac aacaaaaact ttcattatat
     9481 tcctgttatt gaagataaaa gtgaagaatg gatagggaga aaaggcactg ttcttgatgc
     9541 tgtcatggaa gattttactg atcttactta ttttgatatt tatgtttgtg gacccttcat
     9601 gatggctaaa acagcaaaag aaaaattaat tgaagagaaa aaagcaaagt cagaacagat
     9661 gtttgccgat gcttttgcat acgtataata ctagtagcgg ccgctg
//
