LOCUS       sequence-210712-       12274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP reporter of lag-2 driven by 3 kb promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Pekar O, Ow MC, Hui KY, Noyes MB, Hall SE, Hubbard EJA
  TITLE     Linking the environment, DAF-7/TGFbeta signaling and LAG-2/DSL 
            ligand expression in the germline stem cell niche.
  JOURNAL   Development. 2017 Aug 15;144(16):2896-2906. doi: 10.1242/dev.147660.
  PUBMED    28811311
REFERENCE   2  (bases 1 to 12274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2017-08-15- 15"; volume: "144"; 
            issue: "16"; pages: "2896-2906"
COMMENT     Sequence Label: pGC680
FEATURES             Location/Qualifiers
     source          1..12274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1490..1501)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7138..7166)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7914..7934
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(8415..8432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8415..8431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10258..10280
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10318..10336)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10404..10508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10509..10577,10578..11369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10727..10746)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11540..12128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12029..12048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccctgtcg gattatgggc tcttctttta tcctctactt tctttctaga aaatgcaaat
       61 gttgagtcag aaattgctga atggagtaca actgcaatgg gtttagctta tgtttctgga
      121 acaattactg gtggaattgt gattgaaaag taaggttata tttcatttca ataaatgtta
      181 ccacagtata acaaaaacaa gttacattgt tcagagtatg tctgaatgta ttattattat
      241 tgtatttgtg catcagtact gtagttgtac tgaagaaggg tttaaaataa tctcaacgtt
      301 tttaagacga gttagtgaga atacggtcgc agttaggatg tttggacata gttttattag
      361 ataatcgtga actaactcac tataaaaacc cgctaactca ttgaataaga tagttttaaa
      421 cgttcaaaat ttgaagacaa aattgtatga ataagtttac agagttggtc gacgcaagct
      481 tctccttctc ttcacatttc tcaataattt agctctaatt gcattcgtat tcttcgcaaa
      541 aatacgtatt ctgattgacc cgatgaaata cggatgccta gttgcattga tcatctacgg
      601 ttatacttat gggtgaggtt tttcaaatat attttgattt aatcgaataa tgaaatttca
      661 gaactggagt tggtccaatt tcgtggttta tatcctctga actggtacct caaaaacatc
      721 gtagtattgc tcaatctgtg gcatattcaa tgaatactgt aatggtggtt atcagtacat
      781 ttacagtatt acctctatat tccgttattg gtagttatgc tttccttatt ctatattcaa
      841 ttccttcatt tataagtatg cttatattat ttcgctactt accggaaacc aaaggacgag
      901 agattcatga tattgtgaat gaacttaaaa aaaaatgaaa acctttgaaa acgttcatac
      961 ggccgaaatt ttacttgatc gagccaattg aacttttgtt aagacttaca gaaaagtgag
     1021 agattttgtt acaaaaaagt gagaaaaaag cgaaaatgaa caggaaaaag aagaaaaata
     1081 cacccgaagt atcatataaa aattaaaaag attgtttgac ctggcggaac ttttttgcaa
     1141 aaactctgaa tatccatggc acatgcttcg tgcaaaacag gatccaaatg aatatcaaca
     1201 agagattctt gaagacgacg agccacttgc tcagagcatt gcttatctcg aatgagaccc
     1261 ttgttaaaat catttttcaa acattatatg acattgtcga aatgtcctcc tgattccatg
     1321 atggtagcaa actcacttcg tctttgagcc aatttatcca agtccttgta aaaagtattc
     1381 gaaaattgtt aacggataaa atgtttatta taatatcaaa aacaatttgt cagttgacca
     1441 ctttttgata tatttgacag aaaccgggat gaattggctc aaagttaggg cgcccttcta
     1501 ttacagggtt tctgataaca aaccggttat taactcccaa caagggatga tttcaattca
     1561 tcatgctcaa attgacccaa attaagttac atgacaaatt catcgccctt ttcaactctt
     1621 cttggctcat catctgttat tctgttccta ttctgtctgc accctatacc cctttgcata
     1681 ctctctcgtc attcctttct tggatagtgt cttcttctcc ccagctctgc tacttctatg
     1741 acttgcgccc gctgcttttc cgctcgttcc tctcctctct gacgtcttct tctgcttctt
     1801 ttctccttcc tttttccctt ctcaatcttc ctctttttcc catttctgtc aatcattcga
     1861 agaagaagaa gaagaagacc ctcattcatt tcattttttt ttctgtcggt gtgtgctgct
     1921 cggttaagag tgagctctct attccacgtc ttcttctttt tcttcttcga ttcgaatcaa
     1981 tcactccacc aaaacgcatt cgtttttggg attcaccccg cggttcgcaa taggtttctt
     2041 tttcaaatta ttagcgttat aaatagaaaa atggtggagt ttcaataaaa atgataattt
     2101 acaaagtgta ttttgattac atgtactcaa aaaggttgaa atttttcata ccagttttcc
     2161 ggaaatccat ctgatatcat tatcgtattt ttctttttaa aaatgttttc aaaaaaaaac
     2221 aaaatatagc tggttatttg gcaccctcta attaccattt tcctgtcaca ccacactctt
     2281 ttccttcttc cacttctttc cgttttcagc cgcttccaac caaaccgata tgaaagccga
     2341 gcaacaacga tcgattccac ccggctcggc gaccttcccg tcgcaggtga gactcagaaa
     2401 actagagaaa ccgctcaact aactcttgat atccgatttc attcttttct cttttctttt
     2461 tgttgaactt ttccatattt ccagatgcca cggccaccac caagcaccga acaaggaatc
     2521 acaacggaat cggagcttgc gaagaaagct caaatcactc cgaacgacgt tttagcactt
     2581 ccgggaatca ctcaaggtat cttgctccct ttggatttca gtttaaaaca taaatttagg
     2641 attcttatgc tccccatctg cgaacatcta taacatcgag ttcaccaagt tccaaatccg
     2701 tgatctggac actgagcaag tgcttttcga gatcgccaaa ccggagaacg atcaggagaa
     2761 tgatgagtcg ccacaggagt cggcaagata cgtgcgttat agatttgctc caaacttttt
     2821 gaaactcaaa acggtcggag caactgtgga attcaaagta ggagacatcc caatccatca
     2881 tttccgaatg atcgaacgtc acttcttcaa agatcgcctt ctgaagtgtt ttgactttga
     2941 attcggattc tgtattccga attcacgaaa caactgtgaa catatctatg agttccctca
     3001 actctctcaa caactcagtg agtcattatt ctaaaaagta caattcaaaa gactaatctc
     3061 tttttcagtg gacgacatga tcaacaatcc aaacgagact cgttctgaca gcttctattt
     3121 cgtcgataac aaactcgtca tgcacaacaa agccgactac tcatatgatg cataaatatt
     3181 taatacaaaa atgttctgga taattattct gtcgaataga aaaaaaactc caaatgtgat
     3241 taaattccaa taattcctgt ctagtttgtt ccttcccctt ccccttctca tgttcaatgc
     3301 attcctaagc ttttcagttc cccccttgtt tctatatttt ttcgctgtcc tgtcacactc
     3361 gctaaaaaca ctaatcacac ggaaatctgt tttcaataaa aactccaact ttaactcatt
     3421 ttcaatttca actgaaagat tttttcatta gagaatgtct agaactaggc cgtttactgg
     3481 cgctactcca cctttaaatt gcagttgaat tttttttaaa ttctcatttt tggaaaattt
     3541 ggaaatgcac caaaattttg actgaaaatt atgaaacatt tgcaatactt tgaaatttgt
     3601 gtgattcatt tatttaggac agttattagt ttaccttaaa tttccctgaa ctactctact
     3661 ccactttttc caccaaatta ttattcacga acccaaaact atcttacaca tcttatctta
     3721 caggttatac attctgccac gtcttgtaac cccctcccac ctattctcta atatacaacc
     3781 tccctttcaa acagtccgtt atgactctat tgctcctaat cccccttttg tttccggttc
     3841 ggcctgaaac aagaataaca acaggtgact cgataactgt taggaaaata aattttttgg
     3901 tcccaatatt tcggtatcca tttcaaaaca tcttttaaaa ttgtcaagac agacaattac
     3961 ctctgcttga aatgaaggac acatgtcccc tcaattcctc ataaaacaaa tagaggggtg
     4021 tcgaaatgat aaatgttgca agtgaggtgc agatggcaat gaaatttata tatgtttctt
     4081 tcattccatt cttgtggggc aatttcaaac gtttgataac tcacagtggt ctagatttgc
     4141 agaacatctg tgttaggtca agcagagcga gaatttgagc atttgaactt tagggtcctn
     4201 gggggggggg ggtgtctaga cagtcagcgg cccataaggg ttcatatggt ttgacggtgc
     4261 attgaactat tttttaagga aaaaattgag ataaaggcca tttttccaag aaagcatcta
     4321 gctttttcaa ctgacactac aaatttccct ataactttct gaaattatca gtgaaaagtt
     4381 gcccaaattt gagactttag ctaaaaatag acaatttttt ccaaaacttt gagcggccat
     4441 aacttttttt tgaaaaactt tcggaacgtc tcattacaaa atttggttgc tttgagccat
     4501 tttgaatccg aaaaagtctc aaaaagcaaa gtcttaaatt tcggtactcc acctttaaaa
     4561 aaaatagggt ttccgccgaa atttctgggt tccaataata tctgttacca tttccttgct
     4621 ctgcgacgag cagccaccag acaaaaatct cctaataccc ctcttcatgc ctttgttctt
     4681 ccggtttggc ggtctgaaaa agagcacccc tcccggcggg gggctctcgg tatctgttcc
     4741 gcaaaaaccg tcatatggag ggctatcggg agacgggaag agttggaaat acgggaaatg
     4801 gtgatgaata ggtagatcaa tcgattccgg aacaaataat ttgagttgag cacgcgtgct
     4861 ccacatgtgc tcagccgctt aggtgtgagt gaagatcctt ggatttcaag gccatcagcc
     4921 atttcccctg tccagctaga gcagtcctaa acggaatttt ttgcattctt ttgcttcttt
     4981 acaatttcaa atgttcactc taactaattc aaaaatcttt cttttttacg agctctcctt
     5041 gctcgccgtt gtattccatt cataccttta agaccacccg agtgtacgca tatttaacca
     5101 ggcaccctaa aaacatgaat agtctgtggt ggaggtagac agtgtgtttt ctaagaatgt
     5161 ctccgtaatt tgaaatatat tttagagtca cctgaaattc atttttcaag caatatttct
     5221 taccatatct ttggggataa ttttaaaagt ggagtgaagt cgtttaggga cacgctttta
     5281 agttttagga aatggttttt ttttaattca taacttaaaa aaatctataa aaattgttac
     5341 attttttatg attttctcaa ttttgttgaa tgtttgaatt ttttttttga gatgtattgt
     5401 ttgaattgga gcttttttgt gagttttttt ttaattttgc aattaaataa aaagtagcta
     5461 aattagtttt cgaattcccg ctcaaataaa tgtcccagaa tttacatttt tgagttttcc
     5521 ggctaaattt ttgccgtttt ttgaatatga tttgtaatat ttggtctgaa ccatggtttt
     5581 tccaattttc agaatctttc tcgcaaaaaa tctccgtatc ccgcaaaata attgttgaag
     5641 cgtcaaaaat tggcctaaaa gtcgataaat taacattgat ttcgcttttt ttttaggaaa
     5701 aaagtctaaa agtatttttt aaactataca aaatcattaa attttgacaa gaaaacgctt
     5761 aaattacaat tttttttatt ttttaaaaat ttaaaaaaat tttaaaaaaa cattaaaatt
     5821 cagataaatt gcacaatttt tattgcacaa gtcgttcgtc gttcgtgttt ttttcgctgt
     5881 caaaatgttg gcaaagattg tgaagtcccc tgtagtttaa cactctaagt tactccaaag
     5941 acttctacct gcctacctgc ctgcctatct ataacctagt gcctatcacc tactcgctgc
     6001 atagtttgat gtacctatat aacagttcat aaatgaattt tgtcaaaatt ccactctcaa
     6061 gtattcttac acgtacttat tttgtacaaa tccttgtcag tcgctgcaag aacatacaca
     6121 tcgaagaggc gcaatcgaaa caacaggtgt tcgcccgttg atcgtctccc cgccccgcct
     6181 gttgggcggg acgggtgtcg gtcaccacca ccacatcata ttgttggaca cactttgcac
     6241 atccggttca cacccgatta ccgcatcggg gtcttgatct ggggcggcta ttggatcttt
     6301 tgtttatgta gatttttttc tcgccgttat gattttggat tattttttct cttatcttgc
     6361 agtcgacttg taatctttgc ctgattgcta gctagccaaa actttcactt gttctttttt
     6421 ttcctctaag tattttcccg aacttatagt cgaccttgag ctttgctaga agccaataac
     6481 acttttcaaa tttgcccgcg gatccccatc tgcagaattc gcccttaaat gttcactcca
     6541 gaacagttca caaaagtgat gtcccaactg ggtaactttt cccagttggg acaaatgttc
     6601 caacccgatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
     6661 aacatagaac attttcagga ggacccttgg agggtaccgg tccacgggct ccaggatgac
     6721 ccggaccttc aggcccttct gaagggcagc cagcttctga aggtgaagtc cagctcgtgg
     6781 cgtagggaac gcttctacaa gctacaggag gactgcaaga ccatctggca ggaatctcga
     6841 aaggtcatga ggtccccgga gtcgcagctg ttctccatcg aggacattca ggaggtacgg
     6901 atgggacacc gcacagaagg cctggagaag tttgcccgag acatccccga ggatcgatgc
     6961 ttctccattg tcttcaagga ccagcgcaac accctagacc tcattgcccc atcaccagct
     7021 gacgctcagc actgggtgca gggcctgcgc aagatcatcc accactccgg ctccatggac
     7081 cagcggcaga agggaccggt agaaaaaatg agtaaaggag aagaactttt cactggagtt
     7141 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     7201 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     7261 aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc tgattattta
     7321 aattttcagc caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac
     7381 ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     7441 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtaagtt taaacagttc
     7501 ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat
     7561 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     7621 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     7681 aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta
     7741 aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     7801 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     7861 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     7921 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataagga
     7981 tgatcgacgc caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc
     8041 ctattattta tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc
     8101 caaatctaaa catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc
     8161 tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga
     8221 attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat
     8281 gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata
     8341 gaggaggaag gaaaactagt aaaaaataag caaagaaatt aggcgaacga tgagaattgt
     8401 cctcgcttgg aaacactggc cgtcgtttta cactcgagac gtacggtgcg cgcgatgcat
     8461 tcgaagatct gcccactagt gagtcgtatt ataagtgcaa gtaagatcag tgtttgtttc
     8521 gatatcagtc tgtttcgtaa cggtcttctg tataactaca aaaaagaata aaaaaccgta
     8581 tcacaactga gaaaaaagac aaaacttatt gaacagtgtt cctttcaaaa aattattttg
     8641 taaaaaatcg agtttttctc caaaataacg cacgcaatca aaaacaagaa aactaacgtg
     8701 ttctcctggg cgtggaactc cttatcaaga ttaccattat tgtctgtcag aattgatatt
     8761 tcaattattt catatctaga caaacccggg tgtcttgcat ttattagtac tgtgagttta
     8821 catacaaacg aggcttactc agataacata gagtcacctc taattagcaa aaacccattt
     8881 ctaattagct atttcgacca gttctaaagt caccgtaatt agaaatggtt tttgtagctt
     8941 ttctaattag aaatgacccc gagataagtg aaatatggca ttttctgggt tttctagtaa
     9001 gaaatgatcc cgcacgtagt gattccgcgt tacaagccaa attcaaatta aaaaagtata
     9061 ttacacaatt caagttacat tattttcagg aaacaatggc gaagagcaaa gcagagattc
     9121 aaaaggagga aagagacaac agatatgaac gaagacttct tgaaaagaga aagagacatg
     9181 aggtgagtaa ccatgataat aaataatcaa gtaagggcta tagatttata aattctggag
     9241 ttaacagttt cgaaaaaaag tagttttcat atgtgcgaag gaatatcaaa aataggggaa
     9301 tagcctaaac gagtgagaaa attaaatgtt tgttattttt tcaaaaatgt ctgcagggcc
     9361 gttatccttt caaatgcact ctatcaaaca tagatcatat ccatcatatc ttatccagaa
     9421 tttatatcat atccaagatt gagagcgttt caaggaaaat ctcaaacaac catacaattt
     9481 tgtagatcga caacaaggcc tccacttctt catcgatggc ctctgcagca cctgcaccac
     9541 caagtttgta tttttcatat taatataaag tgagccacgc atagtcgttt ttaggatttt
     9601 tgccagatcg atgtcgcgca aacagactgt ttttttttct ttgaaagccg tgcgtgacat
     9661 gggccacgga ttgtaacgcc gcgctggatg gataaaaatt tattcagttt tttagccttt
     9721 acaaaatatg gttcgactac tttctttatt tttacatttt tataggtgct cattgagctg
     9781 agatattttg ggtataatcg gaaaatcgct gattttggtc atttttctgc actaaagatt
     9841 acacaaaaag ttcagtattt agattcattg ttgaaaattg gggatgatct acagatacat
     9901 atcgccgtgt tcaaaaacac acgtgctgtg acgtcgtgac tggggaaaac cctggcgtta
     9961 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    10021 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
    10081 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagtg
    10141 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
    10201 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    10261 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
    10321 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    10381 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10441 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10501 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10561 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10621 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10681 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10741 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10801 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10861 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10921 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10981 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    11041 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    11101 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    11161 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    11221 gtgggtctcg cggtatcatt gcagcactgg ggtcagatgg taagccctcc cgtatcgtag
    11281 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11341 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    11401 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11461 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11521 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11581 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11641 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11701 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11761 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11821 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11881 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11941 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    12001 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    12061 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    12121 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggtcttttgc tggccttttg
    12181 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    12241 agtgagctga taccagcgga taacaatttc acag
//
