LOCUS       sequence-210716-        2142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty plasmid of the pD864-LacZ construct. This is high copy (pUC), 
            AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, 
            Awan AR, Gilbert C, Stan GB, Ellis T
  TITLE     Burden-driven feedback control of gene expression.
  JOURNAL   Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635.
  PUBMED    29578536
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635."
COMMENT     Sequence Label: pD864-LacZ backbone
FEATURES             Location/Qualifiers
     source          1..2142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..97
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      194..782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     683..702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(914..941)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(965..1756,1757..1825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1588..1607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1826..1916)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1925..1955)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(2051..2069)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2065..2112
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcaagctt gatatcattc aggacgagcc tcagactcca gcgtaactgg actgcaatca
       61 actcactggc tcaccttcac gggtgggcct ttcttcggta gaagtcttct catgaccaaa
      121 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      181 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      241 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      301 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      361 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      421 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      481 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      541 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      601 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      661 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      721 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      781 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      841 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      901 ctgataccgc tcgcgcagaa aggcccaccc gaaggtgagc caggtgatta catttgggcc
      961 ctcattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1081 gccccagcgc tgcgatgata ccgcgagaac cacgctcacc ggctccggat ttatcagcaa
     1141 taaaccagcc agcaggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1261 gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1801 cgacacggaa atgttgaata ctcatagctc ctgaaaatct cgataactca aaaaatacgc
     1861 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaagaa
     1921 gacggtcaaa agcctccggt cggaggcttt tgactttctg ctatggaggt caggtatgat
     1981 ttaaatggtc agtattgagc gatatctaga gaattcgtcg ctcttcaggt tagaggtctc
     2041 gcggccgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     2101 aggggttttt tgctgaaagg aggaactata tccgggtaac ga
//
