LOCUS       sequence-210719-       12266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP reporter of lag-2 driven by 1 kb of promoter, which lackes DBS 
            motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Pekar O, Ow MC, Hui KY, Noyes MB, Hall SE, Hubbard EJA
  TITLE     Linking the environment, DAF-7/TGFbeta signaling and LAG-2/DSL 
            ligand expression in the germline stem cell niche.
  JOURNAL   Development. 2017 Aug 15;144(16):2896-2906. doi: 10.1242/dev.147660.
  PUBMED    28811311
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2017-08-15- 15"; volume: "144"; 
            issue: "16"; pages: "2896-2906"
COMMENT     Sequence Label: pGC682
FEATURES             Location/Qualifiers
     source          1..12266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1490..1501)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7130..7158)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7906..7926
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(8407..8424)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8407..8423)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10250..10272
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10310..10328)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10396..10500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10501..10569,10570..11361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10719..10738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11532..12120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12021..12040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccctgtcg gattatgggc tcttctttta tcctctactt tctttctaga aaatgcaaat
       61 gttgagtcag aaattgctga atggagtaca actgcaatgg gtttagctta tgtttctgga
      121 acaattactg gtggaattgt gattgaaaag taaggttata tttcatttca ataaatgtta
      181 ccacagtata acaaaaacaa gttacattgt tcagagtatg tctgaatgta ttattattat
      241 tgtatttgtg catcagtact gtagttgtac tgaagaaggg tttaaaataa tctcaacgtt
      301 tttaagacga gttagtgaga atacggtcgc agttaggatg tttggacata gttttattag
      361 ataatcgtga actaactcac tataaaaacc cgctaactca ttgaataaga tagttttaaa
      421 cgttcaaaat ttgaagacaa aattgtatga ataagtttac agagttggtc gacgcaagct
      481 tctccttctc ttcacatttc tcaataattt agctctaatt gcattcgtat tcttcgcaaa
      541 aatacgtatt ctgattgacc cgatgaaata cggatgccta gttgcattga tcatctacgg
      601 ttatacttat gggtgaggtt tttcaaatat attttgattt aatcgaataa tgaaatttca
      661 gaactggagt tggtccaatt tcgtggttta tatcctctga actggtacct caaaaacatc
      721 gtagtattgc tcaatctgtg gcatattcaa tgaatactgt aatggtggtt atcagtacat
      781 ttacagtatt acctctatat tccgttattg gtagttatgc tttccttatt ctatattcaa
      841 ttccttcatt tataagtatg cttatattat ttcgctactt accggaaacc aaaggacgag
      901 agattcatga tattgtgaat gaacttaaaa aaaaatgaaa acctttgaaa acgttcatac
      961 ggccgaaatt ttacttgatc gagccaattg aacttttgtt aagacttaca gaaaagtgag
     1021 agattttgtt acaaaaaagt gagaaaaaag cgaaaatgaa caggaaaaag aagaaaaata
     1081 cacccgaagt atcatataaa aattaaaaag attgtttgac ctggcggaac ttttttgcaa
     1141 aaactctgaa tatccatggc acatgcttcg tgcaaaacag gatccaaatg aatatcaaca
     1201 agagattctt gaagacgacg agccacttgc tcagagcatt gcttatctcg aatgagaccc
     1261 ttgttaaaat catttttcaa acattatatg acattgtcga aatgtcctcc tgattccatg
     1321 atggtagcaa actcacttcg tctttgagcc aatttatcca agtccttgta aaaagtattc
     1381 gaaaattgtt aacggataaa atgtttatta taatatcaaa aacaatttgt cagttgacca
     1441 ctttttgata tatttgacag aaaccgggat gaattggctc aaagttaggg cgcccttcta
     1501 ttacagggtt tctgataaca aaccggttat taactcccaa caagggatga tttcaattca
     1561 tcatgctcaa attgacccaa attaagttac atgacaaatt catcgccctt ttcaactctt
     1621 cttggctcat catctgttat tctgttccta ttctgtctgc accctatacc cctttgcata
     1681 ctctctcgtc attcctttct tggatagtgt cttcttctcc ccagctctgc tacttctatg
     1741 acttgcgccc gctgcttttc cgctcgttcc tctcctctct gacgtcttct tctgcttctt
     1801 ttctccttcc tttttccctt ctcaatcttc ctctttttcc catttctgtc aatcattcga
     1861 agaagaagaa gaagaagacc ctcattcatt tcattttttt ttctgtcggt gtgtgctgct
     1921 cggttaagag tgagctctct attccacgtc ttcttctttt tcttcttcga ttcgaatcaa
     1981 tcactccacc aaaacgcatt cgtttttggg attcaccccg cggttcgcaa taggtttctt
     2041 tttcaaatta ttagcgttat aaatagaaaa atggtggagt ttcaataaaa atgataattt
     2101 acaaagtgta ttttgattac atgtactcaa aaaggttgaa atttttcata ccagttttcc
     2161 ggaaatccat ctgatatcat tatcgtattt ttctttttaa aaatgttttc aaaaaaaaac
     2221 aaaatatagc tggttatttg gcaccctcta attaccattt tcctgtcaca ccacactctt
     2281 ttccttcttc cacttctttc cgttttcagc cgcttccaac caaaccgata tgaaagccga
     2341 gcaacaacga tcgattccac ccggctcggc gaccttcccg tcgcaggtga gactcagaaa
     2401 actagagaaa ccgctcaact aactcttgat atccgatttc attcttttct cttttctttt
     2461 tgttgaactt ttccatattt ccagatgcca cggccaccac caagcaccga acaaggaatc
     2521 acaacggaat cggagcttgc gaagaaagct caaatcactc cgaacgacgt tttagcactt
     2581 ccgggaatca ctcaaggtat cttgctccct ttggatttca gtttaaaaca taaatttagg
     2641 attcttatgc tccccatctg cgaacatcta taacatcgag ttcaccaagt tccaaatccg
     2701 tgatctggac actgagcaag tgcttttcga gatcgccaaa ccggagaacg atcaggagaa
     2761 tgatgagtcg ccacaggagt cggcaagata cgtgcgttat agatttgctc caaacttttt
     2821 gaaactcaaa acggtcggag caactgtgga attcaaagta ggagacatcc caatccatca
     2881 tttccgaatg atcgaacgtc acttcttcaa agatcgcctt ctgaagtgtt ttgactttga
     2941 attcggattc tgtattccga attcacgaaa caactgtgaa catatctatg agttccctca
     3001 actctctcaa caactcagtg agtcattatt ctaaaaagta caattcaaaa gactaatctc
     3061 tttttcagtg gacgacatga tcaacaatcc aaacgagact cgttctgaca gcttctattt
     3121 cgtcgataac aaactcgtca tgcacaacaa agccgactac tcatatgatg cataaatatt
     3181 taatacaaaa atgttctgga taattattct gtcgaataga aaaaaaactc caaatgtgat
     3241 taaattccaa taattcctgt ctagtttgtt ccttcccctt ccccttctca tgttcaatgc
     3301 attcctaagc ttttcagttc cccccttgtt tctatatttt ttcgctgtcc tgtcacactc
     3361 gctaaaaaca ctaatcacac ggaaatctgt tttcaataaa aactccaact ttaactcatt
     3421 ttcaatttca actgaaagat tttttcatta gagaatgtct agaactaggc cgtttactgg
     3481 cgctactcca cctttaaatt gcagttgaat tttttttaaa ttctcatttt tggaaaattt
     3541 ggaaatgcac caaaattttg actgaaaatt atgaaacatt tgcaatactt tgaaatttgt
     3601 gtgattcatt tatttaggac agttattagt ttaccttaaa tttccctgaa ctactctact
     3661 ccactttttc caccaaatta ttattcacga acccaaaact atcttacaca tcttatctta
     3721 caggttatac attctgccac gtcttgtaac cccctcccac ctattctcta atatacaacc
     3781 tccctttcaa acagtccgtt atgactctat tgctcctaat cccccttttg tttccggttc
     3841 ggcctgaaac aagaataaca acaggtgact cgataactgt taggaaaata aattttttgg
     3901 tcccaatatt tcggtatcca tttcaaaaca tcttttaaaa ttgtcaagac agacaattac
     3961 ctctgcttga aatgaaggac acatgtcccc tcaattcctc ataaaacaaa tagaggggtg
     4021 tcgaaatgat aaatgttgca agtgaggtgc agatggcaat gaaatttata tatgtttctt
     4081 tcattccatt cttgtggggc aatttcaaac gtttgataac tcacagtggt ctagatttgc
     4141 agaacatctg tgttaggtca agcagagcga gaatttgagc atttgaactt tagggtcctg
     4201 gggggggggg ggtgtctaga cagtcagcgg cccataaggg ttcatatggt ttgacggtgc
     4261 attgaactat tttttaagga aaaaattgag ataaaggcca tttttccaag aaagcatcta
     4321 gctttttcaa ctgacactac aaatttccct ataactttct gaaattatca gtgaaaagtt
     4381 gcccaaattt gagactttag ctaaaaatag acaatttttt ccaaaacttt gagcggccat
     4441 aacttttttt tgaaaaactt tcggaacgtc tcattacaaa atttggttgc tttgagccat
     4501 tttgaatccg aaaaagtctc aaaaagcaaa gtcttaaatt tcggtactcc acctttaaaa
     4561 aaaatagggt ttccgccgaa atttctgggt tccaataata tctgttacca tttccttgct
     4621 ctgcgacgag cagccaccag acaaaaatct cctaataccc ctcttcatgc ctttgttctt
     4681 ccggtttggc ggtctgaaaa agagcacccc tcccggcggg gggctctcgg tatctgttcc
     4741 gcaaaaaccg tcatatggag ggctatcggg agacgggaag agttggaaat acgggaaatg
     4801 gtgatgaata ggtagatcaa tcgattccgg aacaaataat ttgagttgag cacgcgtgct
     4861 ccacatgtgc tcagccgctt aggtgtgagt gaagatcctt ggatttcaag gccatcagcc
     4921 atttcccctg tccagctaga gcagtcctaa acggaatttt ttgcattctt ttgcttcttt
     4981 acaatttcaa atgttcactc taactaattc aaaaatcttt cttttttacg agctctcctt
     5041 gctcgccgtt gtattccatt cataccttta agaccacccg agtgtacgca tatttaacca
     5101 ggcaccctaa aaacatgaat agtctgtggt ggaggtagac agtgtgtttt ctaagaatgt
     5161 ctccgtaatt tgaaatatat tttagagtca cctgaaattc atttttcaag caatatttct
     5221 taccatatct ttggggataa ttttaaaagt ggagtgaagt cgtttaggga cacgctttta
     5281 agttttagga aatggttttt ttttaattca taacttaaaa aaatctataa aaattgttac
     5341 attttttatg attttctcaa ttttgttgaa tgtttgaatt ttttttttga gatgtattgt
     5401 ttgaattgga gcttttttgt gagttttttt ttaattttgc aattaaataa aaagtagcta
     5461 aattagtttt cgaattcccg ctcaaataaa tgtcccagaa tttacatttt tgagttttcc
     5521 ggctaaattt ttgccgtttt ttgaatatga tttgtaatat ttggtctgaa ccatggtttt
     5581 tccaattttc agaatctttc tcgcaaaaaa tctccgtatc ccgcaaaata attgttgaag
     5641 cgtcaaaaat tggcctaaaa gtcgataaat taacattgat ttcgcttttt ttttaggaaa
     5701 aaagtctaaa agtatttttt aaactataca aaatcattaa attttgacaa gaaaacgctt
     5761 aaattacaat tttttttatt ttttaaaaat ttaaaaaaat tttaaaaaaa cattaaaatt
     5821 cagataaatt gcacaatttt tattgcacaa gtcgttcgtc gttcgtgttt tttaaaatgt
     5881 tggcaaagat tgtgaagtcc cctgtagttt aacactctaa gttactccaa agacttctac
     5941 ctgcctacct gcctgcctat ctataaccta gtgcctatca cctactcgct gcatagtttg
     6001 atgtacctat ataacagttc ataaatgaat tttgtcaaaa ttccactctc aagtattctt
     6061 acacgtactt attttgtaca aatccttgtc agtcgctgca agaacataca catcgaagag
     6121 gcgcaatcga aacaacaggt gttcgcccgt tgatcgtctc cccgccccgc ctgttgggcg
     6181 ggacgggtgt cggtcaccac caccacatca tattgttgga cacactttgc acatccggtt
     6241 cacacccgat taccgcatcg gggtcttgat ctggggcggc tattggatct tttgtttatg
     6301 tagatttttt tctcgccgtt atgattttgg attatttttt ctcttatctt gcagtcgact
     6361 tgtaatcttt gcctgattgc tagctagcca aaactttcac ttgttctttt ttttcctcta
     6421 agtattttcc cgaacttata gtcgaccttg agctttgcta gaagccaata acacttttca
     6481 aatttgcccg cggatcccca tctgcagaat tcgcccttaa atgttcactc cagaacagtt
     6541 cacaaaagtg atgtcccaac tgggtaactt ttcccagttg ggacaaatgt tccaacccga
     6601 tccccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga
     6661 acattttcag gaggaccctt ggagggtacc ggtccacggg ctccaggatg acccggacct
     6721 tcaggccctt ctgaagggca gccagcttct gaaggtgaag tccagctcgt ggcgtaggga
     6781 acgcttctac aagctacagg aggactgcaa gaccatctgg caggaatctc gaaaggtcat
     6841 gaggtccccg gagtcgcagc tgttctccat cgaggacatt caggaggtac ggatgggaca
     6901 ccgcacagaa ggcctggaga agtttgcccg agacatcccc gaggatcgat gcttctccat
     6961 tgtcttcaag gaccagcgca acaccctaga cctcattgcc ccatcaccag ctgacgctca
     7021 gcactgggtg cagggcctgc gcaagatcat ccaccactcc ggctccatgg accagcggca
     7081 gaagggaccg gtagaaaaaa tgagtaaagg agaagaactt ttcactggag ttgtcccaat
     7141 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     7201 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     7261 tgttccatgg gtaagtttaa acatatatat actaactaac cctgattatt taaattttca
     7321 gccaacactt gtcactactt tctgttatgg tgttcaatgc ttctcgagat acccagatca
     7381 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     7441 tatatttttc aaagatgacg ggaactacaa gacacgtaag tttaaacagt tcggtactaa
     7501 ctaaccatac atatttaaat tttcaggtgc tgaagtcaag tttgaaggtg atacccttgt
     7561 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     7621 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     7681 aatcaaagtt gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca
     7741 gaacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
     7801 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
     7861 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     7921 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataag gatgatcgac
     7981 gccaacgtcg ttgaattttc aaattttaaa tactgaatat ttgttttttt tcctattatt
     8041 tatttattct ctttgtgttt tttttcttgc tttctaaaaa attaattcaa tccaaatcta
     8101 aacatttttt tttctctttc cgtctcccaa ttcgtattcc gctcctctca tctgaacaca
     8161 atgtgcaagt ttatttatct tctcgctttc atttcattag gacgtggggg gaattggtgg
     8221 aagggggaaa cacacaaaag gatgatggaa atgaaataag gacacacaat atgcaacaac
     8281 attcaattca gaaatatgga ggaaggttta aaagaaaaca taaaaatata tagaggagga
     8341 aggaaaacta gtaaaaaata agcaaagaaa ttaggcgaac gatgagaatt gtcctcgctt
     8401 ggaaacactg gccgtcgttt tacactcgag acgtacggtg cgcgcgatgc attcgaagat
     8461 ctgcccacta gtgagtcgta ttataagtgc aagtaagatc agtgtttgtt tcgatatcag
     8521 tctgtttcgt aacggtcttc tgtataacta caaaaaagaa taaaaaaccg tatcacaact
     8581 gagaaaaaag acaaaactta ttgaacagtg ttcctttcaa aaaattattt tgtaaaaaat
     8641 cgagtttttc tccaaaataa cgcacgcaat caaaaacaag aaaactaacg tgttctcctg
     8701 ggcgtggaac tccttatcaa gattaccatt attgtctgtc agaattgata tttcaattat
     8761 ttcatatcta gacaaacccg ggtgtcttgc atttattagt actgtgagtt tacatacaaa
     8821 cgaggcttac tcagataaca tagagtcacc tctaattagc aaaaacccat ttctaattag
     8881 ctatttcgac cagttctaaa gtcaccgtaa ttagaaatgg tttttgtagc ttttctaatt
     8941 agaaatgacc ccgagataag tgaaatatgg cattttctgg gttttctagt aagaaatgat
     9001 cccgcacgta gtgattccgc gttacaagcc aaattcaaat taaaaaagta tattacacaa
     9061 ttcaagttac attattttca ggaaacaatg gcgaagagca aagcagagat tcaaaaggag
     9121 gaaagagaca acagatatga acgaagactt cttgaaaaga gaaagagaca tgaggtgagt
     9181 aaccatgata ataaataatc aagtaagggc tatagattta taaattctgg agttaacagt
     9241 ttcgaaaaaa agtagttttc atatgtgcga aggaatatca aaaatagggg aatagcctaa
     9301 acgagtgaga aaattaaatg tttgttattt tttcaaaaat gtctgcaggg ccgttatcct
     9361 ttcaaatgca ctctatcaaa catagatcat atccatcata tcttatccag aatttatatc
     9421 atatccaaga ttgagagcgt ttcaaggaaa atctcaaaca accatacaat tttgtagatc
     9481 gacaacaagg cctccacttc ttcatcgatg gcctctgcag cacctgcacc accaagtttg
     9541 tatttttcat attaatataa agtgagccac gcatagtcgt ttttaggatt tttgccagat
     9601 cgatgtcgcg caaacagact gttttttttt ctttgaaagc cgtgcgtgac atgggccacg
     9661 gattgtaacg ccgcgctgga tggataaaaa tttattcagt tttttagcct ttacaaaata
     9721 tggttcgact actttcttta tttttacatt tttataggtg ctcattgagc tgagatattt
     9781 tgggtataat cggaaaatcg ctgattttgg tcatttttct gcactaaaga ttacacaaaa
     9841 agttcagtat ttagattcat tgttgaaaat tggggatgat ctacagatac atatcgccgt
     9901 gttcaaaaac acacgtgctg tgacgtcgtg actggggaaa accctggcgt tacccaactt
     9961 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    10021 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
    10081 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tgcaatctgc
    10141 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
    10201 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    10261 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
    10321 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
    10381 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
    10441 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
    10501 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
    10561 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
    10621 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
    10681 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
    10741 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
    10801 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    10861 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
    10921 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    10981 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    11041 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    11101 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    11161 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    11221 cgcggtatca ttgcagcact ggggtcagat ggtaagccct cccgtatcgt agttatctac
    11281 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    11341 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    11401 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    11461 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    11521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    11581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    11641 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    11701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    11761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    11821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    11881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    11941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    12001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    12061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    12121 aacgccagca acgcggcctt tttacggttc ctggtctttt gctggccttt tgctcacatg
    12181 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    12241 gataccagcg gataacaatt tcacag
//
