LOCUS       sequence-210722-       12241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP reporter of lag-2 driven by 1 kb of promoter, which lackes both 
            DBS and Cons motifs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12241)
  AUTHORS   Pekar O, Ow MC, Hui KY, Noyes MB, Hall SE, Hubbard EJA
  TITLE     Linking the environment, DAF-7/TGFbeta signaling and LAG-2/DSL 
            ligand expression in the germline stem cell niche.
  JOURNAL   Development. 2017 Aug 15;144(16):2896-2906. doi: 10.1242/dev.147660.
  PUBMED    28811311
REFERENCE   2  (bases 1 to 12241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2017-08-15- 15"; volume: "144"; 
            issue: "16"; pages: "2896-2906"
COMMENT     Sequence Label: pGC684
FEATURES             Location/Qualifiers
     source          1..12241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1490..1501)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7105..7133)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7881..7901
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(8382..8399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8382..8398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10225..10247
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10285..10303)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10371..10475
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10476..10544,10545..11336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10694..10713)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11507..12095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11996..12015
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccctgtcg gattatgggc tcttctttta tcctctactt tctttctaga aaatgcaaat
       61 gttgagtcag aaattgctga atggagtaca actgcaatgg gtttagctta tgtttctgga
      121 acaattactg gtggaattgt gattgaaaag taaggttata tttcatttca ataaatgtta
      181 ccacagtata acaaaaacaa gttacattgt tcagagtatg tctgaatgta ttattattat
      241 tgtatttgtg catcagtact gtagttgtac tgaagaaggg tttaaaataa tctcaacgtt
      301 tttaagacga gttagtgaga atacggtcgc agttaggatg tttggacata gttttattag
      361 ataatcgtga actaactcac tataaaaacc cgctaactca ttgaataaga tagttttaaa
      421 cgttcaaaat ttgaagacaa aattgtatga ataagtttac agagttggtc gacgcaagct
      481 tctccttctc ttcacatttc tcaataattt agctctaatt gcattcgtat tcttcgcaaa
      541 aatacgtatt ctgattgacc cgatgaaata cggatgccta gttgcattga tcatctacgg
      601 ttatacttat gggtgaggtt tttcaaatat attttgattt aatcgaataa tgaaatttca
      661 gaactggagt tggtccaatt tcgtggttta tatcctctga actggtacct caaaaacatc
      721 gtagtattgc tcaatctgtg gcatattcaa tgaatactgt aatggtggtt atcagtacat
      781 ttacagtatt acctctatat tccgttattg gtagttatgc tttccttatt ctatattcaa
      841 ttccttcatt tataagtatg cttatattat ttcgctactt accggaaacc aaaggacgag
      901 agattcatga tattgtgaat gaacttaaaa aaaaatgaaa acctttgaaa acgttcatac
      961 ggccgaaatt ttacttgatc gagccaattg aacttttgtt aagacttaca gaaaagtgag
     1021 agattttgtt acaaaaaagt gagaaaaaag cgaaaatgaa caggaaaaag aagaaaaata
     1081 cacccgaagt atcatataaa aattaaaaag attgtttgac ctggcggaac ttttttgcaa
     1141 aaactctgaa tatccatggc acatgcttcg tgcaaaacag gatccaaatg aatatcaaca
     1201 agagattctt gaagacgacg agccacttgc tcagagcatt gcttatctcg aatgagaccc
     1261 ttgttaaaat catttttcaa acattatatg acattgtcga aatgtcctcc tgattccatg
     1321 atggtagcaa actcacttcg tctttgagcc aatttatcca agtccttgta aaaagtattc
     1381 gaaaattgtt aacggataaa atgtttatta taatatcaaa aacaatttgt cagttgacca
     1441 ctttttgata tatttgacag aaaccgggat gaattggctc aaagttaggg cgcccttcta
     1501 ttacagggtt tctgataaca aaccggttat taactcccaa caagggatga tttcaattca
     1561 tcatgctcaa attgacccaa attaagttac atgacaaatt catcgccctt ttcaactctt
     1621 cttggctcat catctgttat tctgttccta ttctgtctgc accctatacc cctttgcata
     1681 ctctctcgtc attcctttct tggatagtgt cttcttctcc ccagctctgc tacttctatg
     1741 acttgcgccc gctgcttttc cgctcgttcc tctcctctct gacgtcttct tctgcttctt
     1801 ttctccttcc tttttccctt ctcaatcttc ctctttttcc catttctgtc aatcattcga
     1861 agaagaagaa gaagaagacc ctcattcatt tcattttttt ttctgtcggt gtgtgctgct
     1921 cggttaagag tgagctctct attccacgtc ttcttctttt tcttcttcga ttcgaatcaa
     1981 tcactccacc aaaacgcatt cgtttttggg attcaccccg cggttcgcaa taggtttctt
     2041 tttcaaatta ttagcgttat aaatagaaaa atggtggagt ttcaataaaa atgataattt
     2101 acaaagtgta ttttgattac atgtactcaa aaaggttgaa atttttcata ccagttttcc
     2161 ggaaatccat ctgatatcat tatcgtattt ttctttttaa aaatgttttc aaaaaaaaac
     2221 aaaatatagc tggttatttg gcaccctcta attaccattt tcctgtcaca ccacactctt
     2281 ttccttcttc cacttctttc cgttttcagc cgcttccaac caaaccgata tgaaagccga
     2341 gcaacaacga tcgattccac ccggctcggc gaccttcccg tcgcaggtga gactcagaaa
     2401 actagagaaa ccgctcaact aactcttgat atccgatttc attcttttct cttttctttt
     2461 tgttgaactt ttccatattt ccagatgcca cggccaccac caagcaccga acaaggaatc
     2521 acaacggaat cggagcttgc gaagaaagct caaatcactc cgaacgacgt tttagcactt
     2581 ccgggaatca ctcaaggtat cttgctccct ttggatttca gtttaaaaca taaatttagg
     2641 attcttatgc tccccatctg cgaacatcta taacatcgag ttcaccaagt tccaaatccg
     2701 tgatctggac actgagcaag tgcttttcga gatcgccaaa ccggagaacg atcaggagaa
     2761 tgatgagtcg ccacaggagt cggcaagata cgtgcgttat agatttgctc caaacttttt
     2821 gaaactcaaa acggtcggag caactgtgga attcaaagta ggagacatcc caatccatca
     2881 tttccgaatg atcgaacgtc acttcttcaa agatcgcctt ctgaagtgtt ttgactttga
     2941 attcggattc tgtattccga attcacgaaa caactgtgaa catatctatg agttccctca
     3001 actctctcaa caactcagtg agtcattatt ctaaaaagta caattcaaaa gactaatctc
     3061 tttttcagtg gacgacatga tcaacaatcc aaacgagact cgttctgaca gcttctattt
     3121 cgtcgataac aaactcgtca tgcacaacaa agccgactac tcatatgatg cataaatatt
     3181 taatacaaaa atgttctgga taattattct gtcgaataga aaaaaaactc caaatgtgat
     3241 taaattccaa taattcctgt ctagtttgtt ccttcccctt ccccttctca tgttcaatgc
     3301 attcctaagc ttttcagttc cccccttgtt tctatatttt ttcgctgtcc tgtcacactc
     3361 gctaaaaaca ctaatcacac ggaaatctgt tttcaataaa aactccaact ttaactcatt
     3421 ttcaatttca actgaaagat tttttcatta gagaatgtct agaactaggc cgtttactgg
     3481 cgctactcca cctttaaatt gcagttgaat tttttttaaa ttctcatttt tggaaaattt
     3541 ggaaatgcac caaaattttg actgaaaatt atgaaacatt tgcaatactt tgaaatttgt
     3601 gtgattcatt tatttaggac agttattagt ttaccttaaa tttccctgaa ctactctact
     3661 ccactttttc caccaaatta ttattcacga acccaaaact atcttacaca tcttatctta
     3721 caggttatac attctgccac gtcttgtaac cccctcccac ctattctcta atatacaacc
     3781 tccctttcaa acagtccgtt atgactctat tgctcctaat cccccttttg tttccggttc
     3841 ggcctgaaac aagaataaca acaggtgact cgataactgt taggaaaata aattttttgg
     3901 tcccaatatt tcggtatcca tttcaaaaca tcttttaaaa ttgtcaagac agacaattac
     3961 ctctgcttga aatgaaggac acatgtcccc tcaattcctc ataaaacaaa tagaggggtg
     4021 tcgaaatgat aaatgttgca agtgaggtgc agatggcaat gaaatttata tatgtttctt
     4081 tcattccatt cttgtggggc aatttcaaac gtttgataac tcacagtggt ctagatttgc
     4141 agaacatctg tgttaggtca agcagagcga gaatttgagc atttgaactt tagggtcctg
     4201 gggggggggg ggtgtctaga cagtcagcgg cccataaggg ttcatatggt ttgacggtgc
     4261 attgaactat tttttaagga aaaaattgag ataaaggcca tttttccaag aaagcatcta
     4321 gctttttcaa ctgacactac aaatttccct ataactttct gaaattatca gtgaaaagtt
     4381 gcccaaattt gagactttag ctaaaaatag acaatttttt ccaaaacttt gagcggccat
     4441 aacttttttt tgaaaaactt tcggaacgtc tcattacaaa atttggttgc tttgagccat
     4501 tttgaatccg aaaaagtctc aaaaagcaaa gtcttaaatt tcggtactcc acctttaaaa
     4561 aaaatagggt ttccgccgaa atttctgggt tccaataata tctgttacca tttccttgct
     4621 ctgcgacgag cagccaccag acaaaaatct cctaataccc ctcttcatgc ctttgttctt
     4681 ccggtttggc ggtctgaaaa agagcacccc tcccggcggg gggctctcgg tatctgttcc
     4741 gcaaaaaccg tcatatggag ggctatcggg agacgggaag agttggaaat acgggaaatg
     4801 gtgatgaata ggtagatcaa tcgattccgg aacaaataat ttgagttgag cacgcgtgct
     4861 ccacatgtgc tcagccgctt aggtgtgagt gaagatcctt ggatttcaag gccatcagcc
     4921 atttcccctg tccagctaga gcagtcctaa acggaatttt ttgcattctt ttgcttcttt
     4981 acaatttcaa atgttcactc taactaattc aaaaatcttt cttttttacg agctctcctt
     5041 gctcgccgtt gtattccatt cataccttta agaccacccg agtgtacgca tatttaacca
     5101 ggcaccctaa aaacatgaat agtctgtggt ggaggtagac agtgtgtttt ctaagaatgt
     5161 ctccgtaatt tgaaatatat tttagagtca cctgaaattc atttttcaag caatatttct
     5221 taccatatct ttggggataa ttttaaaagt ggagtgaagt cgtttaggga cacgctttta
     5281 agttttagga aatggttttt ttttaattca taacttaaaa aaatctataa aaattgttac
     5341 attttttatg attttctcaa ttttgttgaa tgtttgaatt ttttttttga gatgtattgt
     5401 ttgaattgga gcttttttgt gagttttttt ttaattttgc aattaaataa aaagtagcta
     5461 aattagtttt cgaattcccg ctcaaataaa tgtcccagaa tttacatttt tgagttttcc
     5521 ggctaaattt ttgccgtttt ttgaatatga tttgtaatat ttggtctgaa ccatggtttt
     5581 tccaattttc agaatctttc tcgcaaaaaa tctccgtatc ccgcaaaata attgttgaag
     5641 cgtcaaaaat tggcctaaaa gtcgataaat taacattgat ttcgcttttt ttttaggaaa
     5701 aaagtctaaa agtatttttt aaactataca aaatcattaa attttgacaa gaaaacgctt
     5761 aaattacaat tttttttatt ttttaaaaat ttaaaaaaat tttaaaaaaa cattaaaatt
     5821 cagataaatt gcacaatttt tattgcacaa gtcgttcgtc gttcgtgttt tttaaaatgt
     5881 tggcaaagat tgtgaagtcc cctgtagttt aacactctaa gttactccaa agacttctac
     5941 ctgcctacct gcctgcctat ctataaccta gtgcctatca cctactcgct gcatagtttg
     6001 atgtacctat ataacagtca ctctcaagta ttcttacacg tacttatttt gtacaaatcc
     6061 ttgtcagtcg ctgcaagaac atacacatcg aagaggcgca atcgaaacaa caggtgttcg
     6121 cccgttgatc gtctccccgc cccgcctgtt gggcgggacg ggtgtcggtc accaccacca
     6181 catcatattg ttggacacac tttgcacatc cggttcacac ccgattaccg catcggggtc
     6241 ttgatctggg gcggctattg gatcttttgt ttatgtagat ttttttctcg ccgttatgat
     6301 tttggattat tttttctctt atcttgcagt cgacttgtaa tctttgcctg attgctagct
     6361 agccaaaact ttcacttgtt cttttttttc ctctaagtat tttcccgaac ttatagtcga
     6421 ccttgagctt tgctagaagc caataacact tttcaaattt gcccgcggat ccccatctgc
     6481 agaattcgcc cttaaatgtt cactccagaa cagttcacaa aagtgatgtc ccaactgggt
     6541 aacttttccc agttgggaca aatgttccaa cccgatcccc gggattggcc aaaggaccca
     6601 aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggagg
     6661 gtaccggtcc acgggctcca ggatgacccg gaccttcagg cccttctgaa gggcagccag
     6721 cttctgaagg tgaagtccag ctcgtggcgt agggaacgct tctacaagct acaggaggac
     6781 tgcaagacca tctggcagga atctcgaaag gtcatgaggt ccccggagtc gcagctgttc
     6841 tccatcgagg acattcagga ggtacggatg ggacaccgca cagaaggcct ggagaagttt
     6901 gcccgagaca tccccgagga tcgatgcttc tccattgtct tcaaggacca gcgcaacacc
     6961 ctagacctca ttgccccatc accagctgac gctcagcact gggtgcaggg cctgcgcaag
     7021 atcatccacc actccggctc catggaccag cggcagaagg gaccggtaga aaaaatgagt
     7081 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     7141 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     7201 acccttaaat ttatttgcac tactggaaaa ctacctgttc catgggtaag tttaaacata
     7261 tatatactaa ctaaccctga ttatttaaat tttcagccaa cacttgtcac tactttctgt
     7321 tatggtgttc aatgcttctc gagataccca gatcatatga aacggcatga ctttttcaag
     7381 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     7441 tacaagacac gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
     7501 ggtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     7561 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     7621 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttgtaag tttaaacatg
     7681 attttactaa ctaactaatc tgatttaaat tttcagaact tcaaaattag acacaacatt
     7741 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     7801 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     7861 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     7921 ggcatggatg aactatacaa ataaggatga tcgacgccaa cgtcgttgaa ttttcaaatt
     7981 ttaaatactg aatatttgtt ttttttccta ttatttattt attctctttg tgtttttttt
     8041 cttgctttct aaaaaattaa ttcaatccaa atctaaacat ttttttttct ctttccgtct
     8101 cccaattcgt attccgctcc tctcatctga acacaatgtg caagtttatt tatcttctcg
     8161 ctttcatttc attaggacgt ggggggaatt ggtggaaggg ggaaacacac aaaaggatga
     8221 tggaaatgaa ataaggacac acaatatgca acaacattca attcagaaat atggaggaag
     8281 gtttaaaaga aaacataaaa atatatagag gaggaaggaa aactagtaaa aaataagcaa
     8341 agaaattagg cgaacgatga gaattgtcct cgcttggaaa cactggccgt cgttttacac
     8401 tcgagacgta cggtgcgcgc gatgcattcg aagatctgcc cactagtgag tcgtattata
     8461 agtgcaagta agatcagtgt ttgtttcgat atcagtctgt ttcgtaacgg tcttctgtat
     8521 aactacaaaa aagaataaaa aaccgtatca caactgagaa aaaagacaaa acttattgaa
     8581 cagtgttcct ttcaaaaaat tattttgtaa aaaatcgagt ttttctccaa aataacgcac
     8641 gcaatcaaaa acaagaaaac taacgtgttc tcctgggcgt ggaactcctt atcaagatta
     8701 ccattattgt ctgtcagaat tgatatttca attatttcat atctagacaa acccgggtgt
     8761 cttgcattta ttagtactgt gagtttacat acaaacgagg cttactcaga taacatagag
     8821 tcacctctaa ttagcaaaaa cccatttcta attagctatt tcgaccagtt ctaaagtcac
     8881 cgtaattaga aatggttttt gtagcttttc taattagaaa tgaccccgag ataagtgaaa
     8941 tatggcattt tctgggtttt ctagtaagaa atgatcccgc acgtagtgat tccgcgttac
     9001 aagccaaatt caaattaaaa aagtatatta cacaattcaa gttacattat tttcaggaaa
     9061 caatggcgaa gagcaaagca gagattcaaa aggaggaaag agacaacaga tatgaacgaa
     9121 gacttcttga aaagagaaag agacatgagg tgagtaacca tgataataaa taatcaagta
     9181 agggctatag atttataaat tctggagtta acagtttcga aaaaaagtag ttttcatatg
     9241 tgcgaaggaa tatcaaaaat aggggaatag cctaaacgag tgagaaaatt aaatgtttgt
     9301 tattttttca aaaatgtctg cagggccgtt atcctttcaa atgcactcta tcaaacatag
     9361 atcatatcca tcatatctta tccagaattt atatcatatc caagattgag agcgtttcaa
     9421 ggaaaatctc aaacaaccat acaattttgt agatcgacaa caaggcctcc acttcttcat
     9481 cgatggcctc tgcagcacct gcaccaccaa gtttgtattt ttcatattaa tataaagtga
     9541 gccacgcata gtcgttttta ggatttttgc cagatcgatg tcgcgcaaac agactgtttt
     9601 tttttctttg aaagccgtgc gtgacatggg ccacggattg taacgccgcg ctggatggat
     9661 aaaaatttat tcagtttttt agcctttaca aaatatggtt cgactacttt ctttattttt
     9721 acatttttat aggtgctcat tgagctgaga tattttgggt ataatcggaa aatcgctgat
     9781 tttggtcatt tttctgcact aaagattaca caaaaagttc agtatttaga ttcattgttg
     9841 aaaattgggg atgatctaca gatacatatc gccgtgttca aaaacacacg tgctgtgacg
     9901 tcgtgactgg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     9961 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    10021 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
    10081 cacaccgcat atggtgcact ctcagtgcaa tctgctctga tgccgcatag ttaagccagc
    10141 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
    10201 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
    10261 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
    10321 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
    10381 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    10441 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10501 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    10561 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    10621 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    10681 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    10741 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10801 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10861 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10921 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10981 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    11041 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    11101 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    11161 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggt
    11221 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    11281 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    11341 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    11401 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    11461 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    11521 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    11581 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    11641 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    11701 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    11761 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11821 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11881 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11941 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    12001 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    12061 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    12121 ggttcctggt cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    12181 ctgtggataa ccgtattacc gcctttgagt gagctgatac cagcggataa caatttcaca
    12241 g
//
