LOCUS       sequence-210743-        8787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_04g08630 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_04g08630-bio-His
FEATURES             Location/Qualifiers
     source          1..8787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3951..3968
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4020..4075
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4074..4093)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6311..6329)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6397..6501
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6502..6570,6571..7362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6720..6739)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7533..8121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8022..8041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8275..8292
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8409..8430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8445..8450,8451..8468,8469..8475)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8483..8499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8488..8510
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8507..8523
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8507..8523
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 acacccccca gaaagtgaac gagcagaaga acgactactt ctacgtggac ggcgtgaagt
     1261 accggaaccc cgccaacgag caccccgcct gcagaggcaa cgtggacctg gtcatcatcg
     1321 tgaagtggcc tggcggcctg gacaagtact cttggagcga gaagatcgcc gagtacctgc
     1381 ccaagttcgt gtcccagctg gaaatcagcg agaaccggtt cagagtgggc ctgatcatga
     1441 gccataccta cgacaaggac ttcctggtgg acctgaacgg cgagcactcc ctggacaaga
     1501 ccaagctgct ggaagaggtg gaaaagtccg tgaacgtggc caacttcgtg atcggcacca
     1561 agctggaaaa gatgctgaat gtggcctacc tgatgttcaa gaagtccaag cggctgaaca
     1621 ccgtgaagat gatctacctg atcagcgacg gcatgtacat cagcgacgaa gaggtcgaga
     1681 aggtgctgaa caaagtgcgg gcatacccaa tccaggtgta cgtgcagggc atcggcgaga
     1741 caagcaagct gtggctgaag ccccacatgg gctgcaccct ggacaacagc tacccctgcc
     1801 ccaacttcat gtacagcaag atcgacgagc ccctggaacc caaggacgcc tacacccgga
     1861 tgtgcctggg catgccccag aacgccgtgt gcttcatcca gtacggcgag ttcaacagct
     1921 gcaagctgcc ctgcagcccc tacagcgtga agtccgccct ggccaccagc tacaccaccc
     1981 tgagaggccc caccttcggc tacgtgggct tcaagcccgg cgtggactgc aagatgcagg
     2041 cccacttcaa ggacatgaag tttaagaagt gcaccctgaa ggactgcatg accgacgagg
     2101 aatacgccga gtatctgaac aacaagaaga agaacaaccg gatcgacatc aagaatgccg
     2161 tgacccccat catggacaag gacaagggca gcaagagcac catcgacgag aagtccccca
     2221 ccggcgagga aaccgacacc agcgacaaga gccagaccga gacaggcgag acagtggaag
     2281 agatcgacga aaccatcacc gacggcaccg aagagacaga cacctccgac aagtcccaga
     2341 cagaaaccgg ggaaaccgtg gaagaaatcg atgagacaat cacagatggg accgaggaaa
     2401 ctgatacctc tgataagagt cagactgaaa ccggcgagac tgtcgaggaa attgacgaga
     2461 caattaccga cgggacagaa gagactgata ccagcgataa gtcccaggcc gagactggaa
     2521 agaccgtgga aaccaccacc aacaacgccg ccgacaccat caacaccgct ggcggcaccg
     2581 ataagagcca ggcagaaaca ggcaaaaccg tcgagacaac cacaaacaat gccgctgata
     2641 caatcaatac tgccggcgga acagacaagt cacagactga gactggcaaa acagtcgaaa
     2701 cgacaactaa caacgctgct gataccatta acacagcagg gggcaccgac aagtctcagg
     2761 ccgaaactgg gaaaactgtc gagactacta ccaacaatgc tgccgacact attaacactg
     2821 ccgggggaac tgataagatc ggccccaacc accccatcaa caacaacgcc ttcaagaccc
     2881 ccatccacaa ggtggaccac aagagccccg agaacgtgga agagagcaaa gagaagcacg
     2941 aggccaacaa gcacgccgac tccaccaagg acaccaacgg cgagaacaag ggcaacgagg
     3001 ccaagaacaa gttcctgagc accgccaaca aagtgaagaa cgccctgttc tacaccgcca
     3061 atacccggaa gctggtgtcc gacaagcccg aaaccatcga gagcaacgtg gccgtgaagt
     3121 ccaccaacca cgccaacaag aacgtggccg gcgacaacaa tgccaacccc aagcacaaca
     3181 acgctgacga agtgatcaag atcaacaacg gccacagcga ggcccacacc gacgacttcg
     3241 agagcagcaa cgacgtgcac accatgagca tcggcaacca gaacgtgaag attgccggtg
     3301 gcgcgccgtc gacctccatc acggcctata agagtgaggg ggagtcagcg gagttctcct
     3361 tcccactcaa ccttggagag gaaagcctgc agggagagtt gagatggaag gcagagaagg
     3421 ctccttcttc ccagtcctgg atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga
     3481 agtctactag caaccccaag ttccagctgt ccgaaacgct cccactcacc cttcagatac
     3541 cccaggtctc ccttcagttt gctggttctg gcaacctgac cctgactctg gacagaggga
     3601 tactgtatca ggaagtgaac ctggtggtga tgaaagtgac tcagcccgac agcaacactt
     3661 tgacctgtga ggtgatggga cccacctcac ccaagatgag actgatcttg aagcaggaga
     3721 atcaggaggc cagggtctcc aggcaggaga aagtgattca agtgcaggcc cctgaagcag
     3781 gggtgtggca atgtctactg agtgaaggtg aagaggtcaa gatggactcc aagatccagg
     3841 ttttatccaa agggttgaat tccggcagcc tgcaccacat cctggacgcc cagaagatgc
     3901 tgtggaacca cagggacagg aacctgcctc ctctggcccc tctgggcccc caccaccatc
     3961 accaccattg agtctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta
     4021 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     4081 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg
     4141 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     4201 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     4261 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     4321 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     4381 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     4441 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     4501 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     4561 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     4621 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     4681 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     4741 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     4801 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     4861 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     4921 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     4981 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     5041 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     5101 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     5161 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     5221 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     5281 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     5341 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     5401 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     5461 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     5521 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     5581 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     5641 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     5701 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     5761 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     5821 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     5881 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     5941 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     6001 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6061 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6121 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     6181 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     6241 tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga
     6301 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6361 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6421 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6481 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6541 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6601 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6661 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6721 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     6781 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6841 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6901 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6961 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7021 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     7081 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7141 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7201 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7261 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7321 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7381 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7441 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7501 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7561 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7621 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7681 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7741 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7801 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7861 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7921 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7981 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8041 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8101 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8161 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8221 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8281 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8341 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8401 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8461 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8521 gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat
     8581 catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga
     8641 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
     8701 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
     8761 atattggctc atgtccaata tgaccgc
//
