LOCUS       sequence-210745-        8277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_04g06950 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_04g06950-bio-His
FEATURES             Location/Qualifiers
     source          1..8277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3441..3458
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3510..3565
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3564..3583)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5801..5819)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5887..5991
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5992..6060,6061..6852)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6210..6229)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7023..7611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7512..7531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7765..7782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7899..7920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7935..7940,7941..7958,7959..7965)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7973..7989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7978..8000
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7997..8013
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7997..8013
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 ccaagaatat cgagcagggc ggcaagcgga acggcctgtt cggctccgac gaagtgggcg
     1261 agatcttcaa gtggatcgtg cggaaccccg ccgacatgca gtgccacgtg atgctggtca
     1321 tctacatcat gctggccagc gagaagcacg acaagaccgc cctgatgacc aaagaaggcg
     1381 tgctgaatat cgccaaggcc atcccctacc tgcccgagca gatcgacgcc aagctgtacg
     1441 gccagctgaa gggcctgaag taccccgaga tgctgctgga aggcatcgag aagatcggca
     1501 atggcctgga cctgcagacc tacatgtaca acaagttcgc cagcgacagc atctgccccc
     1561 ggttcaagga cctggtggac aagcacaact actacgccct gaagcagagc cagcggctgc
     1621 tgggcaaagc catctatatc gccgccctgg cccagcagaa actgatcaac cccaagaagt
     1681 tcagcaagaa ggccttccgg acaatcgcca gcaacctgtt cagcttctgg accttcatcc
     1741 acgacctgcg gtggcggctg cagctggaca tcagcggcac ccccttcgac gtgatgatga
     1801 tcctgaaagt ggggaagatc accatcaaga agaagaacca gctcaacgac ctgaacaacg
     1861 cctacttcga gagcttctac agcctgtaca tccggctgaa gtatgagagc gccctgggcg
     1921 tgttccagga aagcggccac cccatcttcg gcgagttcga caacgtggcc aatgtggccg
     1981 acaccagctt tatccggctc gtgggcttcc tgcacaagaa caaagagggc gtcatctatg
     2041 agtacaactt cggcgtgggc ctgagcgacg tggtcaagaa gcagctgatc gacaagctgg
     2101 aacccaagaa aaccctgctg cagttcatcc tgctggataa gacccacacc aacagcccct
     2161 tctaccacag caaccagtac ttcgacgccg atatgctgta caagatgatc cggaacgagc
     2221 tgtgcgccga cccttacctg atcaatggcg acaaggtgtc catcgtgttc gccgagaaaa
     2281 catacagcac ccaggaattc ctctacaatt tccagcagga cggcggcctg accctggtgc
     2341 tccaggtgtc ccccacccac tacgtgaaca tcgaggaaac catccaggaa ctgcagcgga
     2401 tcgccgctat ccccctgctg accgagatct atcacaagaa tttcgacccc aagcggatca
     2461 gcctgctggg aaactgccac aaaaacgagt atggcgagtt tatgtgcgtg gggaatttcg
     2521 gcaagttcct gggcatgaac atccggtcca agatcgccaa ctacatcaag cccggcagca
     2581 gcgtggtgca gatcctgatc cagggcgctg gcgatatcaa gaacgacaac tgggatgccc
     2641 tgaactccgc cagcattgcc aaccggatca tcagccggat gaagcccacc tacaccgagc
     2701 tgttcatcaa gagagtgcag attctcaaga ccgtggaaga ggaagagaca accatcaacg
     2761 actgcgacaa gtgcagagtg ggctacatcg gcgcgccgtc gacctccatc acggcctata
     2821 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     2881 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     2941 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     3001 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     3061 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     3121 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     3181 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     3241 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     3301 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggcagcc
     3361 tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg aacctgcctc
     3421 ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca cgtgattaat
     3481 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     3541 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     3601 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     3661 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     3721 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     3781 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     3841 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     3901 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     3961 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     4021 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     4081 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     4141 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     4201 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     4261 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     4321 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     4381 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     4441 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     4501 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     4561 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     4621 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     4681 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     4741 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     4801 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     4861 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     4921 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     4981 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     5041 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     5101 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     5161 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     5221 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     5281 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     5341 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     5401 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     5461 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     5521 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     5581 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     5641 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     5701 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     5761 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     5821 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5881 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5941 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6001 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6061 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6121 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6181 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     6241 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6301 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6361 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6421 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6481 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     6541 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6601 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6661 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6721 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6781 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6841 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6901 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6961 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7021 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7081 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7141 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7201 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7261 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7321 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7381 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7441 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7501 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7561 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7621 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7681 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7741 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7801 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7861 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7921 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7981 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     8041 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     8101 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     8161 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     8221 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgc
//
