LOCUS       sequence-210753-        8160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g01156 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g01156-bio-His
FEATURES             Location/Qualifiers
     source          1..8160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3324..3341
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3393..3448
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3447..3466)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5684..5702)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5770..5874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5875..5943,5944..6735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6093..6112)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6906..7494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7395..7414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7648..7665
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7782..7803
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7818..7823,7824..7841,7842..7848)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7856..7872
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7861..7883
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7880..7896
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7880..7896
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 ctctggccaa tgccagaccc gcccaccgga agatcgtgca caagcacaag aacagcggcg
     1261 ccaagccccg gcaccaggcc aagaaacacg tgaacattgc cgaagaggac gacgaggacg
     1321 gcgaggtgta cgaggaagat gaagaggacg attacgagga caccgactac gaggaactgg
     1381 aattcaagcc catcggcgac gagcgggaca acccctggga gagctacatg gaaaagttca
     1441 acatccccaa ggtgcacggc agcggcgtgt acgtggacct gggcaagaac ggcgccttca
     1501 acggcaagaa ataccggatg gtggccggca agtgccccgt gttcggcaag atcatcgagt
     1561 tcagctccgg cgtggactac ctgagccccg ccaacgatgc cgccaacccc gccttcggct
     1621 tcccctatac agtgcctgcc ggcaagacca tccggaccaa cgagatcccc aagaccagcc
     1681 ggggcagaat cagcatcagc aagagcgccg tgcagaccga cctgatcagc cccgtgaccg
     1741 ccaagaccct gaaggcctac gagtacgacg gcgacgacat cttcaactgc gccagctacg
     1801 ccagcgagct gatgatgagc agcgacagaa agagcgacta caagtacccc ttcgccttcg
     1861 acctgaaaac aaagacctgc cacatcctgt acagccccct gcagctgatc cagggcccca
     1921 agtactgcga caacgacggc aaggtggaca gcggcagcag cagcatgccc tgcatcaagc
     1981 ccgtgaagga catgagccag gaaatggtct acggcagcag cttcatctac cgggactgga
     2041 agaacaagtg ccccaacgcc gccgtggccg acgccatctt tggcacctgg aatggcgccg
     2101 cttgcgtgcc catccagaac cggcggctgt tcaaggccag cacccccgag atctgcggcc
     2161 agatcgtgtt caagtacagc gccagcgacg cccccgagaa ctacgagaca aagcggagcg
     2221 agggcagcaa gttcgccaac gccatcagca gcggcgatct gggcgccgtg gccaagatca
     2281 tcatgcccgt gaccaacagc agagcccacc acagcaaggg ctggggcttc aactgggcca
     2341 attacgaccg gaacaagaga gagtgcggcc tgatcgacga ggtgcccaac tgcctggtgt
     2401 tcaagatggg caatatcgcc ttcaacagcc tgggcagccc cctggaagat gacatggaaa
     2461 acttcccttg cgagatcaag agcttcggct acatcaccaa gggcaacccc aacgacatca
     2521 acagctacct gatctccagc acccaccggg acatgatccc caacgacgac ggcatcgaga
     2581 cactgaacgc cagaagctgc agcggctact acggcaagat cggagccaaa gagagcgaaa
     2641 ccctgaacca ggcccccctg accttctacg gtggcgcgcc gtcgacctcc atcacggcct
     2701 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     2761 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     2821 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     2881 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     2941 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     3001 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     3061 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     3121 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     3181 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggca
     3241 gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac aggaacctgc
     3301 ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag acacgtgatt
     3361 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     3421 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     3481 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     3541 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     3601 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     3661 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     3721 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     3781 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     3841 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     3901 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     3961 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     4021 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     4081 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     4141 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     4201 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     4261 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     4321 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     4381 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     4441 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     4501 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     4561 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     4621 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     4681 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     4741 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     4801 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     4861 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     4921 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     4981 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     5041 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     5101 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     5161 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     5221 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     5281 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     5341 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     5401 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     5461 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     5521 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     5581 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     5641 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     5701 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5761 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5821 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5881 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5941 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6001 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6061 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     6121 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6181 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6241 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6301 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6361 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     6421 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6481 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6541 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6601 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6661 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6721 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6781 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6841 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6901 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6961 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7021 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7081 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7141 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7201 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7261 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7321 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7381 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7441 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7501 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7561 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7621 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7681 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7741 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7801 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7861 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     7921 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     7981 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     8041 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     8101 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
//
