LOCUS       sequence-210763-        8469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g02875 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g02875-bio-His
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3633..3650
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3702..3757
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3756..3775)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5993..6011)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6079..6183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6184..6252,6253..7044)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6402..6421)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7215..7803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7704..7723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7957..7974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8091..8112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8127..8132,8133..8150,8151..8157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8165..8181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8170..8192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8189..8205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8189..8205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 tcggcggcag cttcatccag agcaacagcg acaagaccaa gaaccggtgc aagggcctgg
     1261 aatacctggg catcggctac gacgccatcg tgcccaagct gatcgccgag gacgagatca
     1321 acaccgacct gggctacaga taccccatcc tgcggcagca ctacggctcc gactacagct
     1381 acgccagcac cggctgcgtg taccccgtgg acgcctacta ccggcccttc ctgagctgcc
     1441 agcggcagga aagcttctcc cagatcggca gcgtggccgc ctatgagaac gcattcggga
     1501 tccaggccaa gaccgacttc aaagtgaagt ccgtggaatt cgccgccagc gcccagttcg
     1561 agaagtccag caagaacctg gaagagaaca agtaccgcga gatcatccac atcatcaact
     1621 gcttccaggc cgagatgggc ctgcccacca acctgcagtg gggcatcaag cccgagttca
     1681 acaaggccgt gctgaagctg accgaggcct tcggcatcag cgccaccgcc agcaacgacg
     1741 gcagcatctg cacccccaag agctacaaga accccggcag acactgccgg gaagtgcggc
     1801 ggtggatccg gctgatcaag tcctacggca cccactacgt gcacaagatc cggatcggcg
     1861 ggaagctggt gcagagcgtg cggatcagcc agatctctat cgccaacatg gaagaacagg
     1921 gcatcgacgt ggaaggcgag atccgggccg aaatgctgag catcacaggc agcggcggag
     1981 gcaagtccaa caagctgaca gatgagatcg acaagaacgc catccggaag atcagagtga
     2041 tcggaggcat catgcctaag ctgccactga ccccccaggc cttcaagaag tgggcccgga
     2101 ccctggacag caaccccatg cccatctatg tggaagtgga ccccctgtac aagatcatcg
     2161 agcagagcct gaacaacgac aaccctatcc ccttcaacat caagcggcag tacgacatgg
     2221 ccctggactt ctacgccgag ctgaagggcg ccacctacaa gcagatcatg gaaatgaacg
     2281 gcaacatccc cagcatcagc cacctgatcc ggaacagcgc catggtcacc aacaacgccc
     2341 tgaagcggag caccctgagg tgcaagaacg atgccaacat cgtggccggc gtgaccatct
     2401 tcttcgacga cgccggcaag ggcatccacc tgttcccttg caagagcggc atgaacgagt
     2461 gcaacgtgcc cgagatcagc gaggacgtgg cctacacttg ggcctgggcc ctgtgcagcc
     2521 acagactgct gcccagcatt gagcaggtcg tgcggggcga tttcaaaaag ggggagatca
     2581 agtgccccga gggctataag atgggcttcg gcctggcctt taaagaaaac atggcctact
     2641 atatcaacgg ctacatctcc gccatctctg ccgacgacaa caatgccgcc gtgtccctgc
     2701 tggacggcaa cgccggctgg atcacctgtt tcgacaagga catggtgcag gacatgtaca
     2761 ccgtggtcaa catcgaccac agcaacaagc gggtggtggc cgagtgcccc agcgacagca
     2821 tcatcatcgg gggcgtgaag atctccaagg cccctcccgg gggaggcgag gaaggggatc
     2881 atctggccag agccgaggaa tgcgacaagt tcgtgaccag ctgcaccgcc ggacccccag
     2941 ccggcttcgt gtggctgatc tgcggcgata aggccgacga gggcgcgccg tcgacctcca
     3001 tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc aaccttggag
     3061 aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct tcccagtcct
     3121 ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact agcaacccca
     3181 agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc tcccttcagt
     3241 ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat caggaagtga
     3301 acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt gaggtgatgg
     3361 gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag gccagggtct
     3421 ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg caatgtctac
     3481 tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc aaagggttga
     3541 attccggcag cctgcaccac atcctggacg cccagaagat gctgtggaac cacagggaca
     3601 ggaacctgcc tcctctggcc cctctgggcc cccaccacca tcaccaccat tgagtctaga
     3661 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     3721 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     3781 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     3841 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     3901 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     3961 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     4021 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     4081 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     4141 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     4201 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     4261 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     4321 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     4381 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     4441 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     4501 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     4561 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     4621 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     4681 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     4741 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     4801 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     4861 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     4921 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     4981 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     5041 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     5101 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     5161 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     5221 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     5281 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     5341 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     5401 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     5461 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     5521 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     5581 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
     5641 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
     5701 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
     5761 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     5821 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     5881 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     5941 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
     6001 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6061 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6121 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6181 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6241 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6301 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6361 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6421 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6481 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6541 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6601 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6661 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6721 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6781 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6841 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6901 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6961 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7021 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7081 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7141 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7321 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7981 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8041 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8101 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8161 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     8221 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     8281 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     8341 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     8401 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     8461 tatgaccgc
//
