LOCUS       sequence-210764-        9222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g01875 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 9222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g01875-bio-His
FEATURES             Location/Qualifiers
     source          1..9222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4386..4403
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4455..4510
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4509..4528)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6746..6764)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6832..6936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6937..7005,7006..7797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7155..7174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7968..8556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8457..8476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8710..8727
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8844..8865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8880..8885,8886..8903,8904..8910)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8918..8934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8923..8945
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8942..8958
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8942..8958
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 ccaccaacga gaacgagaga caggacggct tcgtggacaa gatgttcaag aacggcaacg
     1261 agtggaagcg gttctgcacc ctgttcaagc ccgaggacaa gaagcggccc ttcacccact
     1321 gcagcgtgaa catgcacccc cacgagacag tgggaatcaa gtgcccccgg ttcctgcacc
     1381 ggaactacga catcttccca ctgcaggaaa accacatcta caccagcgag acaagcgtga
     1441 acaccaacga agtgaccgac cccgccatct tcaaccccga ggaactgaac ctgatcggca
     1501 agcacctgag cagcgtgtac gacaaggacg gctacaacgt gatcctgagc aacggcggca
     1561 ccaacgacaa cctgaagtcc ctgtactaca tctgcggcgt gcccagcatg gacaccgacg
     1621 actacgagaa catcgacaac gtgggcatca tgctggccct gatcaacatc gtgctgctgc
     1681 cccccaacca ggaagataac acccggtact acaacatcca cctggacacc aaccccatcg
     1741 tgtccccata tcaggacgtg tccatgatca gagaggtgga ctactacggc atctacaaga
     1801 tcgactgcgg caaagaaacc atcaacgtgc agaaccccgt gtccagcacc gtgcggagcg
     1861 ccagctacgt gcccagcgag aagttcaaca agaacagcct gaacgaggtg gtcaaggccc
     1921 tgaccagagc cggcaacgag aagtccgtgg aaagcaccgc cagcagcagc caccccagca
     1981 acgtgatcac catcaacggc ctggatatcc tgaagtccat caagagcgac cccgacaaga
     2041 tcgtgaagct gaactgccag acccaccaca gcatcgtgtt cgccagcgcc aacgagcagc
     2101 acttcagccc cgagggcatc atcagccaga ccctgctgct gatccccgac aagtcctaca
     2161 ccagaagcaa cagcctgacc ttcagcatcc ccgtgcacct gaaggccccc atctgcagcc
     2221 ccacccccga taagagcccc gagaactaca tctacgacga ccggatcctg tgcaagtaca
     2281 atgccgccac caccaccttc agcaccctgt actgccccag agagacacac accttcgaca
     2341 tcaagacctg caagcggagc ttccagacct ggcagaacta ccggaccaac aagatgatct
     2401 tcgacaagga cttcaacgtg atcgccgtga ccgacgagta ctacatgttc ctgtacaaga
     2461 acctggccgc tcctttcacc cagaagctga tctgcagctg ccagcccaag caggaagtga
     2521 cagaggccaa cgaggtgtcc atcagcatca cccccaagct ggactgcgac ttcaccgacc
     2581 ccaaccccgc cagcgtggga atcatcaaca acgcctgcaa ccgggtcgtg cggcctggcg
     2641 agacaatcgt ggtcatctgc cgcgagggcg acgtgatcta ccccaagaag ggcctggcct
     2701 acttcggcaa caccgtgttc cctttcgacg acatcgactt cgagggcacc aagcggagcg
     2761 acctgaaagt ggtgtccgtg ggcaacgaca agttcctgat caccctgccc agcgatacca
     2821 agatcttcct gggccagcag tacgtgttca gctgcagcag cggcaagggc atccgggaag
     2881 tgggcttccc catcaaggac accggcgtgg tcatcatcac caccggcaac cccaacttcc
     2941 cccagagcat cgacaagaag ctgcctaccc tggtcaagaa gctgaagcag aacgagtacg
     3001 gctactacaa cctgaaactg agctacggcg atctggaagt cgagtgcaag aactacctgc
     3061 acgacaccaa tgagaacaca gccgccctgt accccatcaa ctgggccgac atgtacagcg
     3121 cccagagcaa gcggggcatc gaccccagaa ccatgcacgt ggtgcacccc aaccagttca
     3181 tgggcttcca cggcatcagc ttcaagaaac tgcccgacaa gcagaagctg cccagcatcc
     3241 tgtccgtgtc tatcaagaac gacgccattg tgctgcacgg ccggcagtac cccctgtact
     3301 tcgtgtgcat ggacctgctg gaagatagcg tgtgggacgg cgacaagggc agattcgccg
     3361 tgttcgagat cgagttcgac ctggaccaca aagaaatggt cggatgcggc gtgtacccca
     3421 agatgtttaa ggatggcaga gggaacgtga tgaccaacaa caactgcgag ttcgacatga
     3481 acatccacga caaagtgggc tttcactgcc ccctgagcaa tgagaacccc atcccccccg
     3541 tgtgctacgc cggcgacatc gataagaagt ccggcacccg gaaggtggtg gaaatcttca
     3601 agcggcccac cgacctgtac gagttccggt ccctgtggat cttcaccaag gacatgctga
     3661 agtacgccgc caacggcgcc atcgagtgca gctgcatcag ccagggcgag acactggcca
     3721 ccatcaccat ctctggcgcg ccgtcgacct ccatcacggc ctataagagt gagggggagt
     3781 cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga gagttgagat
     3841 ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta aagaaccaaa
     3901 aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa acgctcccac
     3961 tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac ctgaccctga
     4021 ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc
     4081 ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag atgagactga
     4141 tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg attcaagtgc
     4201 aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag gtcaagatgg
     4261 actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac cacatcctgg
     4321 acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg gcccctctgg
     4381 gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg atcccccgac
     4441 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     4501 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     4561 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     4621 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     4681 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     4741 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     4801 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     4861 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     4921 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     4981 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     5041 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     5101 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     5161 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     5221 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     5281 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     5341 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     5401 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     5461 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     5521 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     5581 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     5641 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     5701 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     5761 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     5821 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     5881 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     5941 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     6001 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     6061 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     6121 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     6181 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6241 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     6301 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     6361 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     6421 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     6481 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     6541 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     6601 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     6661 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     6721 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     6781 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6841 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6901 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6961 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7021 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7081 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7141 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     7201 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7261 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7321 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7381 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7441 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     7501 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7561 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7621 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7681 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7741 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7801 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7861 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7921 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7981 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8041 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8101 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8161 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8221 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8281 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8341 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8401 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8461 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8521 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8581 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8641 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8701 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8761 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8821 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     8881 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8941 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     9001 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     9061 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
     9121 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
     9181 ataatatgta catttatatt ggctcatgtc caatatgacc gc
//
