LOCUS       sequence-210765-        8562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g00435 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g00435-bio-His
FEATURES             Location/Qualifiers
     source          1..8562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3726..3743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3795..3850
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3849..3868)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6086..6104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6172..6276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6277..6345,6346..7137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6495..6514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7308..7896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7797..7816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8050..8067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8184..8205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8220..8225,8226..8243,8244..8250)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8258..8274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8263..8285
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8282..8298
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8282..8298
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 acatcggcgc ctatggctct ggccccgacg aggacaccca cgacgccacc cagcccgaga
     1261 acaagaacat cgacctgagc gacggcgaga acctgcacac cgagctgaga cacggccagg
     1321 ccgagcccga gggcagcaac ctgcagaccg acgacagcat ctacaccagc acccccgagc
     1381 agcccagcga cgtgtccggc agccctaagg tggacgacac cgagagcgac tacgccgtga
     1441 aggccggcca gagcgacagc gtgaacgcca actacgaccc caccaacgac ttcaccctgg
     1501 gcagcgcccc tgagatcaag atcgagtgcg acaacaacct ggaatacggc gtggtcaact
     1561 acttcccact gaccaagctg atgaagttct acattatgta cgagaaagag ctggaaaacg
     1621 tcatcatcag ccagaagctg accagcagct ctatcgccga gcccttctgc aagaagctga
     1681 tcgagctgac cacctgtctg ctgaagcaga tcgagttcaa catggaccgg atcgacagct
     1741 accgggccca cctgaaccgg atcatcgacg agaacaacgc cgccatcgac gagttcccca
     1801 tcagcgccga cttcatcaag gcccgggtgt ccgccttcca cggcttcttc atcatcggcg
     1861 agcggacctg gaagctgctg aacctggcca agaaccaggc caccatcctg cggagagtga
     1921 ccagcatcct gagcagcaga ctgctgaaaa ccagcgccag cagcctgtac cggaccatca
     1981 acctggaaca tctgctgctg cccagcagca acttcgccct ggacatcagc agcctggccc
     2041 atctgaagaa agaactggac gataccgtgg ccaagaagta cctgctgaag atcatctacg
     2101 acgcctacaa ccgggccgtg gacttcgaga acaacttcaa gaccttctcc agcttcctgc
     2161 ggatcagcaa acaggtgctg agcgacaccg acaaagaaca cttccacaag gtggcctacg
     2221 acctgatcat cgacttcgag cagctgatca gagtgcggaa cctgggcaac tgcctgaagt
     2281 ccaccctggg cgaggaaggc aacgacgaga acggcgctcc cctgagcgag atcgacgcct
     2341 accccatcag caccttcggc gactgcccca acaagctgga acccctggac gagtacgcca
     2401 tcaagaacag catcgaggac ttcaccggcc tgctggaaat ctgcctgaaa acccccacca
     2461 gccccagcga caccaccctg tccgaggacg ccaatctggg caaggacgcc ggcatcggca
     2521 aggacaccaa cctgcgggaa gtgaacaaca tcggaaagga tgccaacctg ggaaaagatg
     2581 ccggaatcga taaggacaag acactgagcg aggacaagaa tatcgggaaa gacgctaacc
     2641 tggggaagga tgcaggcatt gacaaggata agaccctctc agaggataag aacattggca
     2701 aggatgctaa tcttgggaag gacgcaggga tcgacaaaga caaaaccctc agtgaagata
     2761 agaatattgg aaaggacgca aacctcggca aagacgcagg tatagacaag gacaaaactc
     2821 tgtctgagga caaaaacatt ggtaaagatg caaatctcgg caaagatgct gggatagaca
     2881 aagataagac actgtcagaa gataagaaca tcggaaaaga cgccaatctc ggtaactacg
     2941 ccaacctgcc caacagcatc tatctgcagg ccaagaaaac cgtgcgggac ctgatcggca
     3001 gcgacagcgc caagatcaac gccctgctgc acttcctggt gccatttcaa gtcgggatgc
     3061 tgatcatgag cctgggcgcg ccgtcgacct ccatcacggc ctataagagt gagggggagt
     3121 cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga gagttgagat
     3181 ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta aagaaccaaa
     3241 aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa acgctcccac
     3301 tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac ctgaccctga
     3361 ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc
     3421 ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag atgagactga
     3481 tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg attcaagtgc
     3541 aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag gtcaagatgg
     3601 actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac cacatcctgg
     3661 acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg gcccctctgg
     3721 gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg atcccccgac
     3781 ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     3841 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga tccctcggag
     3901 atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     3961 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     4021 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     4081 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     4141 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     4201 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     4261 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     4321 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     4381 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     4441 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     4501 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     4561 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     4621 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     4681 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     4741 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     4801 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     4861 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     4921 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     4981 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     5041 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     5101 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     5161 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     5221 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     5281 aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg ccaagatagg
     5341 ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag cgccaagcac
     5401 agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta atgttgccat
     5461 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     5521 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     5581 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     5641 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     5701 agcatatgct atcctaatag agattagggt agtatatgct atcctaattt atatctgggt
     5761 agcatatact acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca
     5821 tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca
     5881 tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca
     5941 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     6001 tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag ctgtcaaaca
     6061 tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     6121 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6181 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6241 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6301 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6361 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6421 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6481 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     6541 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6601 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6661 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6721 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6781 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     6841 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6901 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6961 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7021 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7081 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7141 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7201 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7321 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7381 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7441 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7501 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7561 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7621 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7681 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7741 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7801 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7861 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7921 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7981 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8041 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8101 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8161 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     8221 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8281 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga ccaattctca
     8341 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
     8401 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
     8461 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
     8521 ataatatgta catttatatt ggctcatgtc caatatgacc gc
//
