LOCUS       sequence-210767-        8091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_01g00425 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_01g00425-bio-His
FEATURES             Location/Qualifiers
     source          1..8091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3255..3272
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3324..3379
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3378..3397)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5615..5633)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5701..5805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5806..5874,5875..6666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6024..6043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6837..7425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7326..7345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7579..7596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7713..7734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7749..7754,7755..7772,7773..7779)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7787..7803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7792..7814
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7811..7827
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7811..7827
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 tgcacatcga gtgccacacc ctgagaaccc tgaacgacgc caaccccaag aacaccgcca
     1261 accccgccag acccagcacc ctggaaaccg gcaacgacaa ccccgtgaac aacgtgcccg
     1321 tgtccgacga ccccgccgac aacgcccctg ccaacaactt cggcaacacc gccaccggca
     1381 ccctgcgcga gcacgtggaa aaccggcgga aggccatgat cggcagcgac agcaccaaca
     1441 gcgccgacga cgagcagcag atgcacagcc ccctgaacag cccccggtac aagagccggt
     1501 tcagcgccgt gcgcaagacc ctgaatgacg agctgggcat catcctgaac aacaagctga
     1561 agcagcacaa gcagggcgac ccccgcgagg tggtggacca gaaccaggtg cccgacaaca
     1621 gcctgacccc tgccgataac tccatcaagc tgggcgaccg ggatgccggc gagtctgcca
     1681 cagtgcctgc caaggacgcc accaacatcg acgtgaacaa tgccctggaa ctgggcagat
     1741 tcagcatgta ccccctgtac aagctgtact tcgccgacga ctacgagaag aacaccaacc
     1801 tgaacggcga caccctgaag tccctggacc tggtggaaat gcaggtcaag tacaacatcg
     1861 agcagctcga gaacatgcgc gagctgatca ttaagctgga cgagagcctg cagaagctga
     1921 acgccaagat ccccgacgac ggcaccatcc ggcagttcaa cgccttctgc aacaagatca
     1981 agaagttcgg caagccctgg atcaacagct acgagctgac caagggcaag ctggtgcagc
     2041 tgaacggccg gatcaagctg atcaaagagt ctatcgccga gggcagagtg ggcatcaaga
     2101 acatgatctt ccagctgttc aagagcgtgg gaatcaacct ggtggacggc ggcatcctgg
     2161 ccgacggcaa cgccatcctg aacgatgaga acatcgacaa gttcaccgac tacctgaaca
     2221 agctgatccg cggcaagctg gaaatcgaca gcctgaagat gaactgctac gccatccaga
     2281 acacccaggt ggaactgtac cagctgctga gcacccactt tctggacagc cccgtggacc
     2341 ccgagaaccc tctggtgcct gccggcctga tccccgagag caacaccgac agcggcaaga
     2401 acaaggccaa caacctgaag aaaaagagca tgatccgcga gcggagagag atgcagatca
     2461 acggcaatct gaagaacagc ggcagcgact ccgacagcga cgacagcatc ggctaccagc
     2521 ccgagctgaa gcggctgacc cagctgcaga gctacgagga caacaacgac gccctggacc
     2581 agagcgagga cagcgacagc tctggcgcgc cgtcgacctc catcacggcc tataagagtg
     2641 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     2701 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     2761 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     2821 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     2881 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     2941 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     3001 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     3061 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     3121 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc agcctgcacc
     3181 acatcctgga cgcccagaag atgctgtgga accacaggga caggaacctg cctcctctgg
     3241 cccctctggg cccccaccac catcaccacc attgagtcta gacacgtgat taattaagga
     3301 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     3361 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     3421 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     3481 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     3541 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     3601 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3661 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3721 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     3781 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     3841 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     3901 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     3961 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     4021 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     4081 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     4141 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     4201 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     4261 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     4321 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     4381 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     4441 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     4501 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     4561 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     4621 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4681 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4741 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     4801 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     4861 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     4921 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     4981 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     5041 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     5101 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     5161 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     5221 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     5281 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     5341 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     5401 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     5461 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5521 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     5581 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5641 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5701 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5761 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5821 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5881 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5941 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6001 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     6061 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6121 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6181 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6241 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6301 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     6361 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6421 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6481 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6541 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6601 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6661 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6721 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6781 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6841 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6901 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6961 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7021 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7081 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7141 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7201 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7261 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7321 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7381 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7441 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7501 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7561 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7621 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7681 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7741 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7801 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     7861 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     7921 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     7981 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     8041 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg c
//
