LOCUS       sequence-210774-        5906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5906)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..5906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        163..366
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     317..337
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      405..414
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             411..1091
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     polyA_signal    2757..2878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2794..2813)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2848..2867
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2885..3340)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3022..3043)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3234..3253
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3367..3471
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3496..3516)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3540..3750
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3562..3758
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3609..3744
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3793..4587
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3793..4587
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3847..3866)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4457..4476
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4775..4794)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4819..4866
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5195..5783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5684..5703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      121 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      181 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      241 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      301 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      361 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagtg
      421 cgattaagcc agacatgagg atcaaactcc gtatggaagg caacgtaaac gggcaccact
      481 ttgtgatcga cggagatggt acaggcaagc cttatgaggg aaaacagacc atggatcttg
      541 aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt
      601 acggcaacag ggtattcgtg aaatatccag acaacataca agactatttt aagcagtcgt
      661 ttcctaaggg gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcaatg
      721 ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga ttttatggta
      781 ccaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca
      841 ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga tattgagatg gctttgttgc
      901 ttgaaggaaa tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg
      961 gtgtcaagtt accaggcgcc cactttgtgg accacgccat tgagatttta agccatgaca
     1021 aagattacaa caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca
     1081 atgccagacg atccggaagt gctgggagcg caagcggaag cggaggctcc ggttttaact
     1141 acgaacgtcc aaaacacttc atccagtccc aaaacccatg tggctccaga ttgcagcctc
     1201 ctggaccaga aacctccagc ttctctagcc agaccaaaca gtcttccatt atcatccagc
     1261 cccgccagtg tacagagcaa agattttctg cctcctcaac actgagctct cacatcacca
     1321 tgtcctcctc tgctttccct gcttctcccc agcagcatgc tggctccaac ccaggccaaa
     1381 gggttacaac cacctataac cagtccccag ccagcttcct cagctccata ttaccatcac
     1441 agcctgatta caatagcagt aaaatccctt ccgctatgga ttccaactat caacagtcct
     1501 cagctggcca acctataaat gcaaagccat cccaaactgc aaatgctaag cccataccaa
     1561 gaactcctga tcatgaaata caaggatcaa aagaagcttt gattcaagat ttggaaagaa
     1621 agctgaaatg caaggacacc cttcttcata atggaaatca acgtctaaca tatgaagaga
     1681 agatggctcg cagattgcta ggaccacaga atgcagctgc tgtgtttcaa gctcaggatg
     1741 acagtggtgc acaagactcg cagcaacaca actcagaaca tgcgcgactg caagttccta
     1801 catcacaagt aagaagtaga tcaacctcaa ggggagatgt gaatgatcag gatgcaatcc
     1861 aggagaaatt ttacccacca cgtttcattc aagtgccaga gaacatgtcg attgatgaag
     1921 gaagattctg cagaatggac ttcaaagtga gtggactgcc agctcctgat gtgtcatggt
     1981 atctaaatgg aagaacagtt caatcagatg atttgcacaa aatgatagtg tctgagaagg
     2041 gtcttcattc actcatcttt gaagtagtca gagcttcaga tgcaggggct tatgcatgtg
     2101 ttgccaagaa tagagcagga gaagccacct tcactgtgca gctggatgtc cttgcaaaag
     2161 aacataaaag agcaccaatg tttatctaca aaccacagag caaaaaagtt ttagagggag
     2221 attcagtgaa actagaatgc cagatctcgg ctatacctcc accaaagctt ttctggaaaa
     2281 gaaataatga aatggtacaa ttcaacactg accgaataag cttatatcaa gataacactg
     2341 gaagagttac tttactgata aaagatgtaa acaagaaaga tgctgggtgg tatactgtgt
     2401 cagcagttaa tgaagctgga gtgactacat gtaacacaag attagacgtt acggcacgtc
     2461 caaaccaaac tcttccagct cctaagcagt tacgggttcg accaacattc agcaaatatt
     2521 tagcacttaa tgggaaaggt ttgaatgtaa aacaagcttt taacccagaa ggagaatttc
     2581 agcgtttggc agctcaatct ggactctatg aaagtgaaga actttaagga tccaccggat
     2641 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     2701 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     2761 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     2821 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     2881 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2941 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3001 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3061 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3121 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3181 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3241 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3301 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     3361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3421 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     3481 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3541 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3601 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3661 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     3721 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     3781 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     3841 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     3901 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     3961 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4021 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4081 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4141 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4201 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4261 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4321 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4381 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4441 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4501 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4561 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     4621 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     4681 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     4741 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     4801 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     4861 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     4921 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     4981 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     5041 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5101 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5161 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5221 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5281 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5341 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5401 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5461 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5521 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5581 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5641 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5701 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5761 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5821 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5881 gtattaccgc catgcattag ttatta
//
