LOCUS       sequence-210777-        7888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation:400 / 490, Emission:468 / 
            511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7888)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Alpha-5-Integrin-12
FEATURES             Location/Qualifiers
     source          1..7888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3891..4607
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    4730..4851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4767..4786)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4821..4840
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4858..5313)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4995..5016)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5207..5226
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5340..5444
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5446..5803
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5446..5637
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5469..5489)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5654..5789
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5838..6632
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5838..6632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5892..5911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6502..6521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6820..6839)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6864..6911
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7240..7828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7729..7748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      661 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccaccatg gggagccgga
      721 cgccagagtc ccctctccac gccgtgcagc tgcgctgggg cccccggcgc cgacccccgc
      781 tgctgccgct gctgttgctg ctgctgccgc cgccacccag ggtcgggggc ttcaacttag
      841 acgcggaggc cccagcagta ctctcggggc ccccgggctc cttcttcgga ttctcagtgg
      901 agttttaccg gccgggaaca gacggggtca gtgtgctggt gggagcaccc aaggctaata
      961 ccagccagcc aggagtgctg cagggtggtg ctgtctacct ctgtccttgg ggtgccagcc
     1021 ccacacagtg cacccccatt gaatttgaca gcaaaggctc tcggctcctg gagtcctcac
     1081 tgtccagctc agagggagag gagcctgtgg agtacaagtc cttgcagtgg ttcggggcaa
     1141 cagttcgagc ccatggctcc tccatcttgg catgcgctcc actgtacagc tggcgcacag
     1201 agaaggagcc actgagcgac cccgtgggca cctgctacct ctccacagat aacttcaccc
     1261 gaattctgga gtatgcaccc tgccgctcag atttcagctg ggcagcagga cagggttact
     1321 gccaaggagg cttcagtgcc gagttcacca agactggccg tgtggtttta ggtggaccag
     1381 gaagctattt ctggcaaggc cagatcctgt ctgccactca ggagcagatt gcagaatctt
     1441 attaccccga gtacctgatc aacctggttc aggggcagct gcagactcgc caggccagtt
     1501 ccatctatga tgacagctac ctaggatact ctgtggctgt tggtgaattc agtggtgatg
     1561 acacagaaga ctttgttgct ggtgtgccca aagggaacct cacttacggc tatgtcacca
     1621 tccttaatgg ctcagacatt cgatccctct acaacttctc aggggaacag atggcctcct
     1681 actttggcta tgcagtggcc gccacagacg tcaatgggga cgggctggat gacttgctgg
     1741 tgggggcacc cctgctcatg gatcggaccc ctgacgggcg gcctcaggag gtgggcaggg
     1801 tctacgtcta cctgcagcac ccagccggca tagagcccac gcccaccctt accctcactg
     1861 gccatgatga gtttggccga tttggcagct ccttgacccc cctgggggac ctggaccagg
     1921 atggctacaa tgatgtggcc atcggggctc cctttggtgg ggagacccag cagggagtag
     1981 tgtttgtatt tcctgggggc ccaggagggc tgggctctaa gccttcccag gttctgcagc
     2041 ccctgtgggc agccagccac accccagact tctttggctc tgcccttcga ggaggccgag
     2101 acctggatgg caatggatat cctgatctga ttgtggggtc ctttggtgtg gacaaggctg
     2161 tggtatacag gggccgcccc atcgtgtccg ctagtgcctc cctcaccatc ttccccgcca
     2221 tgttcaaccc agaggagcgg agctgcagct tagaggggaa ccctgtggcc tgcatcaacc
     2281 ttagcttctg cctcaatgct tctggaaaac acgttgctga ctccattggt ttcacagtgg
     2341 aacttcagct ggactggcag aagcagaagg gaggggtacg gcgggcactg ttcctggcct
     2401 ccaggcaggc aaccctgacc cagaccctgc tcatccagaa tggggctcga gaggattgca
     2461 gagagatgaa gatctacctc aggaacgagt cagaatttcg agacaaactc tcgccgattc
     2521 acatcgctct caacttctcc ttggaccccc aagccccagt ggacagccac ggcctcaggc
     2581 cagccctaca ttatcagagc aagagccgga tagaggacaa ggctcagatc ttgctggact
     2641 gtggagaaga caacatctgt gtgcctgacc tgcagctgga agtgtttggg gagcagaacc
     2701 atgtgtacct gggtgacaag aatgccctga acctcacttt ccatgcccag aatgtgggtg
     2761 agggtggcgc ctatgaggct gagcttcggg tcaccgcccc tccagaggct gagtactcag
     2821 gactcgtcag acacccaggg aacttctcca gcctgagctg tgactacttt gccgtgaacc
     2881 agagccgcct gctggtgtgt gacctgggca accccatgaa ggcaggagcc agtctgtggg
     2941 gtggccttcg gtttacagtc cctcatctcc gggacactaa gaaaaccatc cagtttgact
     3001 tccagatcct cagcaagaat ctcaacaact cgcaaagcga cgtggtttcc tttcggctct
     3061 ccgtggaggc tcaggcccag gtcaccctga acggtgtctc caagcctgag gcagtgctat
     3121 tcccagtaag cgactggcat ccccgagacc agcctcagaa ggaggaggac ctgggacctg
     3181 ctgtccacca tgtctatgag ctcatcaacc aaggccccag ctccattagc cagggtgtgc
     3241 tggaactcag ctgtccccag gctctggaag gtcagcagct cctatatgtg accagagtta
     3301 cgggactcaa ctgcaccacc aatcacccca ttaacccaaa gggcctggag ttggatcccg
     3361 agggttccct gcaccaccag caaaaacggg aagctccaag ccgcagctct gcttcctcgg
     3421 gacctcagat cctgaaatgc ccggaggctg agtgtttcag gctgcgctgt gagctcgggc
     3481 ccctgcacca acaagagagc caaagtctgc agttgcattt ccgagtctgg gccaagactt
     3541 tcttgcagcg ggagcaccag ccatttagcc tgcagtgtga ggctgtgtac aaagccctga
     3601 agatgcccta ccgaatcctg cctcggcagc tgccccaaaa agagcgtcag gtggccacag
     3661 ctgtgcaatg gaccaaggca gaaggcagct atggcgtccc actgtggatc atcatcctag
     3721 ccatcctgtt tggcctcctg ctcctaggtc tactcatcta catcctctac aagcttggat
     3781 tcttcaaacg ctccctccca tatggcaccg ccatggaaaa agctcagctc aagcctccag
     3841 ccacctctga tgccacggta ccgcgggccc gggatccacc ggtcgccacc atgagcaagg
     3901 gcgccgagct gttcaccggc atcgtgccca tcctgatcga gctgaatggc gatgtgaatg
     3961 gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4021 tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg gtggccaccc
     4081 tgagctacgg cgtgcagtgc ttctcacgct accccgatca catgaagcag cacgacttct
     4141 tcaagagcgc catgcctgag ggctacatcc aggagcgcac catcttcttc gaggatgacg
     4201 gcaactacaa gacgcgcgcc gaggtgaagt tcgagggcga taccctggtg agtcgcatcg
     4261 agctgaccgg cactgatttc aaggaggatg gcaacatcct gggcaataag atggagtaca
     4321 actacaacgc caccaatgtg tacatcgtgg ccgacaaggc caggaatggc atcaaggtga
     4381 acttcaaggt ccgccacaac atcaaggatg gcagcgtgca gctggccgac cactaccagc
     4441 agaatacccc catcggcgat ggccctgtgc tgctgcccga taaccactac ctgtccaccc
     4501 agagcgccct gtccaaggac cccaacgaga agcgcgatca catgatctac ctcgagttcg
     4561 tgaccgccgc cgccatcacc cacggcatgg atgagctgta caagtaaagc ggccgcgact
     4621 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     4681 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     4741 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4801 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     4861 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4921 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4981 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     5041 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     5101 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5161 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5221 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5281 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     5341 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5401 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     5461 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5521 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5581 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5641 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5701 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5761 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     5821 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     5881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6001 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6361 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     6661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     6721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6781 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     6841 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     6901 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     6961 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     7021 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     7081 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7381 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7861 ggccttttgc tggccttttg ctcacatg
//
