LOCUS       sequence-210787-        6087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6087)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Vimentin-C-18
FEATURES             Location/Qualifiers
     source          1..6087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      661..670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             667..1347
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     polyA_signal    2929..3050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2966..2985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3020..3039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3057..3512)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3194..3215)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3406..3425
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3539..3643
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3645..4002
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3645..3836
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3668..3688)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3853..3988
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4037..4831
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4037..4831
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4091..4110)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4701..4720
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5019..5038)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5063..5110
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5439..6027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5928..5947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc
      661 gccaccatgg tgagtgcgat taagccagac atgaggatca aactccgtat ggaaggcaac
      721 gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagcctta tgagggaaaa
      781 cagaccatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg
      841 accactgcat tccattacgg caacagggta ttcgtgaaat atccagacaa catacaagac
      901 tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac
      961 gggggcattt gcaatgccag aaacgacata acaatggaag gggacacttt ctataataaa
     1021 gttcgatttt atggtaccaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg
     1081 aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac gggtgatatt
     1141 gagatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac
     1201 aaagctaagg agaagggtgt caagttacca ggcgcccact ttgtggacca cgccattgag
     1261 attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat
     1321 tctggattgc ctgacaatgc cagacgatcc ggactcagat ctggcagcgg tggaggcagc
     1381 gcatccggcg gaagcggaag ctccaccagg tccgtgtcct cgtcctccta ccgcaggatg
     1441 ttcggcggcc cgggcaccgc gagccggccg agctccagcc ggagctacgt gactacgtcc
     1501 acccgcacct acagcctggg cagcgcgctg cgccccagca ccagccgcag cctctacgcc
     1561 tcgtccccgg gcggcgtgta tgccacgcgc tcctctgccg tgcgcctgcg gagcagcgtg
     1621 cccggggtgc ggctcctgca ggactcggtg gacttctcgc tggccgacgc catcaacacc
     1681 gagttcaaga acacccgcac caacgagaag gtggagctgc aggagctgaa tgaccgcttc
     1741 gccaactaca tcgacaaggt gcgcttcctg gagcagcaga ataagatcct gctggccgag
     1801 ctcgagcagc tcaagggcca aggcaagtcg cgcctggggg acctctacga ggaggagatg
     1861 cgggagctgc gccggcaggt ggaccagcta accaacgaca aagcccgcgt cgaggtggag
     1921 cgcgacaacc tggccgagga catcatgcgc ctccgggaga aattgcagga ggagatgctt
     1981 cagagagagg aagccgaaaa caccctgcaa tctttcagac aggatgttga caatgcgtct
     2041 ctggcacgtc ttgaccttga acgcaaagtg gaatctttgc aagaagagat tgcctttttg
     2101 aagaaactcc acgaagagga aatccaggag ctgcaggctc agattcagga acagcatgtc
     2161 caaatcgatg tggatgtttc caagcctgac ctcacggctg ccctgcgtga cgtacgtcag
     2221 caatatgaaa gtgtggctgc caagaacctg caggaggcag aagaatggta caaatccaag
     2281 tttgctgacc tctctgaggc tgccaaccgg aacaatgacg ccctgcgcca ggcaaagcag
     2341 gagtccactg agtaccggag acaggtgcag tccctcacct gtgaagtgga tgcccttaaa
     2401 ggaaccaatg agtccctgga acgccagatg cgtgaaatgg aagagaactt tgccgttgaa
     2461 gctgctaact accaagacac tattggccgc ctgcaggatg agattcagaa tatgaaggag
     2521 gaaatggctc gtcaccttcg tgaataccaa gacctgctca atgttaagat ggcccttgac
     2581 attgagattg ccacctacag gaagctgctg gaaggcgagg agagcaggat ttctctgcct
     2641 cttccaaact tttcctccct gaacctgagg gaaactaatc tggattcact ccctctggtt
     2701 gatacccact caaaaaggac acttctgatt aagacggttg aaactagaga tggacaggtt
     2761 atcaacgaaa cttctcagca tcacgatgac cttgaataag gatccaccgg atctagataa
     2821 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2881 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2941 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3001 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
     3061 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3121 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3181 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3241 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3301 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3361 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3421 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3481 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3541 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3601 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     3661 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3721 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3781 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3841 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3901 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3961 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     4021 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4081 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4141 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4201 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4261 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4321 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4381 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4441 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4501 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4561 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4621 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     4681 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     4741 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     4801 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     4861 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4921 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4981 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     5041 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     5101 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     5161 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5221 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     5281 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5341 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5581 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6061 gccttttgct ggccttttgc tcacatg
//
