LOCUS       sequence-210794-       11673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gateway destination vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11673)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UBC-Myc-gate-pGK-HYG
FEATURES             Location/Qualifiers
     source          1..11673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    224..341
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        433..1644
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1481..1500
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1690..1699
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1699..1728
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1750..1874
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1899..1904,1905..1922,1923..1929)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1983..2663
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(2049..2068)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2983..3288
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3167..3186
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3329..3453)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3597..4096
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3632..3655)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4029..4048
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4114..5139
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4114..5139
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(5170..5203)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5259..5847
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5312..5332)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5730..5741)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5850..5866)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5851..5867)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6056..6289
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6361..6482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6398..6417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6452..6471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6522..6657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6677..6696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6678..6696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6719..7077)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6759..6780)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6971..6990
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7104..7208
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7210..7567
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7210..7401
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7233..7253)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7418..7553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7602..8396
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7602..8396
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7656..7675)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8266..8285
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8584..8603)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8628..8675
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9004..9592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9493..9512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9767..10070
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10072..10270
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10225..10245
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10288..10468
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10515..10640
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11137..11370
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11555..11599
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
       61 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
      121 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
      181 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
      241 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      301 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
      361 gcagagatcc agtttggtta gtaccgggcc cgctctagcc ctagatttaa cccaacagaa
      421 ggctcgacgc gtggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
      481 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
      541 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
      601 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
      661 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
      721 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
      781 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
      841 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
      901 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
      961 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     1021 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     1081 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     1141 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     1201 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     1261 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     1321 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     1381 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     1441 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     1501 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     1561 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     1621 ccgtttttgg cttttttgtt agacgctaga ctgccggatc gaattgtcga cgaattcgga
     1681 tccagatctg ccaccatgga gcagaagctg atctctgagg aggatctggg aggcgctcga
     1741 ggttatcaaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     1801 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     1861 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     1921 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     1981 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     2041 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     2101 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     2161 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     2221 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     2281 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     2341 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     2401 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     2461 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     2521 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     2581 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag gcggggcgta
     2641 atctagagga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     2701 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     2761 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     2821 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     2881 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     2941 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     3001 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     3061 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     3121 ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     3181 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     3241 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     3301 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     3361 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     3421 cgtttctcgt tcagctttct tgtacaaagt ggttcgataa cgaattccgc cccccccccc
     3481 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     3541 ttccaccata ttgccgtctt ttggcaatgt gagggccgcg tttaacgaat tctaccgggt
     3601 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     3661 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     3721 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     3781 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     3841 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     3901 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     3961 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     4021 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     4081 atctccgggc ctttcgacct gcatcccgcc accatgaaaa agcctgaact caccgcgacg
     4141 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     4201 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     4261 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     4321 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     4381 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     4441 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     4501 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     4561 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     4621 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     4681 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     4741 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     4801 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     4861 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     4921 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     4981 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     5041 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     5101 aaccgacgcc ccagcactcg tccgagggca aaggaatagt gtacaagtag gaattcgtcg
     5161 agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt taagggttcc
     5221 ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     5281 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5341 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5401 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5461 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5521 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5581 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5641 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5701 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5761 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5821 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg agacctagaa
     5881 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     5941 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     6001 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     6061 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6301 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6361 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6421 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6481 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     6541 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6601 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6661 ggacgtaccc aattcgccct atagtgagtc gtattagcat gtgagcaaaa ggccagcata
     6721 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     6781 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     6841 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     6901 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     6961 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     7021 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     7081 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7141 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7201 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     7261 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7321 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7381 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7441 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7501 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7561 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     7621 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7681 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7741 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     7801 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     7861 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     7921 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     7981 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     8041 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     8101 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     8161 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     8221 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     8281 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     8341 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     8401 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     8461 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     8521 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     8581 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     8641 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     8701 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     8761 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     8821 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     8881 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8941 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9001 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9061 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9121 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9181 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9241 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9301 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9361 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9421 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9481 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9541 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9601 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9661 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt
     9721 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     9781 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     9841 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     9901 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     9961 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    10021 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
    10081 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
    10141 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
    10201 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
    10261 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
    10321 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10381 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10441 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
    10501 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
    10561 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
    10621 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
    10681 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
    10741 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
    10801 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
    10861 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
    10921 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
    10981 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    11041 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    11101 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    11161 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    11221 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    11281 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    11341 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    11401 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    11461 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    11521 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    11581 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    11641 attattcata atgatagtag gaggcttggt agg
//
