LOCUS       sequence-210795-        6005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 507 / 572, Emission: 516 / 
            580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6005)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-Cx43-7
FEATURES             Location/Qualifiers
     source          1..6005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2045..2725
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2847..2968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2884..2903)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2938..2957
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2975..3430)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3112..3133)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3324..3343
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3457..3561
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3563..3920
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3563..3754
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3586..3606)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3771..3906
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3955..4749
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3955..4749
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4009..4028)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4619..4638
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4937..4956)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4981..5028
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5357..5945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5846..5865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      661 tcagatctcg agctcaagct tcgaattcgg cagcactttt ctttcattgg ggggaaggcg
      721 tgaggaaagt accaaacagc agcagacttt taaactttaa acagacaggt ctgagagcct
      781 gaactctcat ttttcctttg acttcagcct ccaaggagtt ccaccaactt tggcgcgccg
      841 gcttcacttt cattaagtga aagagaggtg cccagacatg ggtgactgga gtgccttggg
      901 gaagcttctg gacaaggtcc aagcctactc caccgctgga gggaaggtgt ggctgtcagt
      961 gctcttcata ttcagaatcc tgctcctggg gacagctgtt gagtcagctt ggggtgatga
     1021 acagtctgcc tttcgctgta acactcaaca acctggctgc gaaaacgtct gctatgacaa
     1081 gtccttcccc atctctcacg tgcgcttctg ggtccttcag atcatattcg tgtctgtgcc
     1141 cacactcctg tacttggccc atgtgttcta tgtgatgagg aaggaagaga agctaaacaa
     1201 gaaagaagag gagctcaaag tggcccagac tgacggggtc aacgtggaga tgcacctgaa
     1261 gcagattgaa atcaagaagt tcaagtacgg gattgaagag cacggcaagg tgaaaatgag
     1321 gggcggcttg ctgagaacct acatcatcag catcctcttc aagtctgtct tcgaggtggc
     1381 cttcctgctc atccagtggt acatctatgg gttcagcttg agcgcggtct acacctgcaa
     1441 gagagatccc tgcccccacc aggtagactg cttcctctca cgtcccacgg agaaaaccat
     1501 cttcatcatc ttcatgctgg tggtgtcctt ggtgtctctc gctttgaaca tcattgagct
     1561 cttctacgtc ttcttcaagg gcgttaagga tcgcgtgaag ggaagaagcg atccttacca
     1621 cgccaccact ggcccactga gcccatcaaa agactgcgga tctccaaaat acgcctactt
     1681 caatggctgc tcctcaccaa cggctccact ctcgcctatg tctcctcctg ggtacaagct
     1741 ggttactggt gacagaaaca attcctcgtg ccgcaattac aacaagcaag ctagcgagca
     1801 aaactgggcg aactacagcg cagagcaaaa tcgcatgggg caggccggaa gcaccatctc
     1861 caactcgcac gcccagccgt tcgatttccc cgacgacaac cagaatgcca aaaaagttgc
     1921 tgctggacat gaactccagc cattagccat cgtggaccaa cgaccttcca gcagagccag
     1981 cagccgcgcc agcagcaggc ctcggcctga tgacctggag attgcggatc caccggtcgc
     2041 caccatgagt gcgattaagc cagacatgaa gatcaaactc cgtatggaag gcaacgtaaa
     2101 cgggcaccac tttgtgatcg acggagatgg tacaggcaag ccttttgagg gaaaacagag
     2161 tatggatctt gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac
     2221 tgcattccat tacggcaaca gggtattcgc caaatatcca gacaacatac aagactattt
     2281 taagcagtcg tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg aagacggggg
     2341 catttgcaac gccagaaacg acataacaat ggaaggggac actttctata ataaagttcg
     2401 attttatggt accaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg
     2461 ggagccctcc actgagaaaa tgtatgtgcg tgatggagtg ctgacgggtg atattgagat
     2521 ggctttgttg cttgaaggaa atgcccatta ccgatgtgac ttcagaacta cttacaaagc
     2581 taaggagaag ggtgtcaagt taccaggcgc ccactttgtg gaccactgca ttgagatttt
     2641 aagccatgac aaagattaca acaaggttaa gctgtatgag catgctgttg ctcattctgg
     2701 attgcctgac aatgccagac gataagcggc cgcgactcta gatcataatc agccatacca
     2761 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     2821 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     2881 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2941 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     3001 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3061 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3121 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3181 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     3241 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3301 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     3361 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3421 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     3481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     3541 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     3601 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3661 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3721 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3781 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3841 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3901 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     3961 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4021 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4081 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     4141 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4201 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4261 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4321 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4381 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4441 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     4501 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4561 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4621 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4681 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     4741 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     4801 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     4861 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     4921 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     4981 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     5041 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     5101 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     5161 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     5221 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5281 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5341 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5401 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5461 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5521 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5581 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5641 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5701 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5761 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5821 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5881 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5941 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6001 acatg
//
