LOCUS       sequence-210807-        5536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 
            / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5536)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-LASP2-C-18
FEATURES             Location/Qualifiers
     source          1..5536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             667..1344
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2378..2499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2415..2434)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2469..2488
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2506..2961)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2643..2664)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2855..2874
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2988..3092
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3094..3451
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3094..3285
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3117..3137)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3302..3437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3486..4280
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3486..4280
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3540..3559)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4150..4169
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4468..4487)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4512..4559
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4888..5476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5377..5396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc
      661 gccaccatga gtgcgattaa gccagacatg aagatcaaac tccgtatgga aggcaacgta
      721 aacgggcacc actttgtgat cgacggagat ggtacaggca agccttttga gggaaaacag
      781 agtatggatc ttgaagtcaa agagggcgga cctctgcctt ttgcctttga tatcctgacc
      841 actgcattcc attacggcaa cagggtattc gccaaatatc cagacaacat acaagactat
      901 tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt cgaagacggg
      961 ggcatttgca acgccagaaa cgacataaca atggaagggg acactttcta taataaagtt
     1021 cgattttatg gtaccaactt tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa
     1081 tgggagccct ccactgagaa aatgtatgtg cgtgatggag tgctgacggg tgatattgag
     1141 atggctttgt tgcttgaagg aaatgcccat taccgatgtg acttcagaac tacttacaaa
     1201 gctaaggaga agggtgtcaa gttaccaggc gcccactttg tggaccactg cattgagatt
     1261 ttaagccatg acaaagatta caacaaggtt aagctgtatg agcatgctgt tgctcattct
     1321 ggattgcctg acaatgccag acgatccgga ctcagatctc gagagtccag tggatattcg
     1381 tctcggtcgg gtggaagcaa cccccagtgc gcccgctgtg ggaaagtggt ctatcccacc
     1441 gagaaagtca actgcctgga caagtattgg cataaaggat gtttccactg tgaagtttgc
     1501 aaaatggctc tgaacatgaa taactacaaa ggatatgaaa agaaacctta ttgcaatgca
     1561 cattacccaa agcagtcctt cactacagtg gctgatactc cagaaaatct acgtctgaag
     1621 caacaaagtg aattgcagag tcaggtaaaa tacaaaagag actttgaaga aagcaaagga
     1681 agagggttca gtattgtgac cgatacgccc gagttacaaa gactgaaaag gactcaggaa
     1741 caaatcagta atgttaaata ccatgaagat tttgagaaga caaagggcag gggtttcacg
     1801 ccagtggtgg atgatcctat aacagagcgg gtgcggaaaa acacccaagt tgtaagtgat
     1861 gcagcgtata aaggggtgca cccacatata gttgaaatgg atagaagacc tggaataatt
     1921 gttgcccctg tactccctgg ggcatatcag caaagccagt cccaaggata tggatacatg
     1981 caccagacca gtatgtcatc tatgaggtca gtgcattcac agcctcatcc agcaggcctg
     2041 agaacataca gagctatgta tgactacagt gctcaagatg aagatgaagt ttccttcagg
     2101 gatggtgact atatcatcaa cgtgcaacca attgatgatg gctggatgta tggtactgtt
     2161 cagagaactg ggaaaacagg aatgcttcca gcaaattata ttgagtttgt taactaagaa
     2221 ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
     2281 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     2341 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     2401 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2461 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
     2521 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     2581 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     2641 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     2701 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     2761 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     2821 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     2881 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     2941 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3001 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3061 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     3121 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3181 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     3241 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3301 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3361 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3421 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     3481 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     3541 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     3601 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     3661 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     3721 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     3781 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     3841 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     3901 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     3961 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     4021 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     4081 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     4141 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     4201 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     4261 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     4321 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     4381 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     4441 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     4501 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     4561 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     4621 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     4681 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     4741 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4801 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4921 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4981 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5041 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5101 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5161 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5221 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5281 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5341 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5401 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5461 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5521 gccttttgct cacatg
//
