LOCUS       sequence-210823-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gal-Estradiol transcription activation expression cassette marked 
            with KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Costa EA, Subramanian K, Nunnari J, Weissman JS
  TITLE     Defining the physiological role of SRP in protein-targeting 
            efficiency and specificity.
  JOURNAL   Science. 2018 Jan 18. pii: science.aar3607. doi: 
            10.1126/science.aar3607.
  PUBMED    29348368
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Jan 18. pii: science.aar3607. doi: 10.1126/science.aar3607."
COMMENT     Sequence Label: pJW1663
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..939
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             52..942
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
ORIGIN
        1 tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
       61 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
      121 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
      181 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
      241 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
      301 gcctggctag agatcctgat gattggtctc gtctggcgct ccatggagca cccagtgaag
      361 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
      421 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
      481 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
      541 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
      601 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
      661 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
      721 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
      781 gagatgctgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
      841 gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag
      901 tattacatca cgggggaggc agagggtttc cctgccacag tc
//
