LOCUS       sequence-210844-       14093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of mRIN2 dRHVPS9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14093)
  AUTHORS   Sandri C, Caccavari F, Valdembri D, Camillo C, Veltel S, 
            Santambrogio M, Lanzetti L, Bussolino F, Ivaska J, Serini G
  TITLE     The R-Ras/RIN2/Rab5 complex controls endothelial cell adhesion and 
            morphogenesis via active integrin endocytosis and Rac signaling.
  JOURNAL   Cell Res. 2012 Oct;22(10):1479-501. doi: 10.1038/cr.2012.110. Epub 
            2012 Jul 24.
  PUBMED    22825554
REFERENCE   2  (bases 1 to 14093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2012.110"; journalName: "Cell Res"; date: "2012-10"; 
            volume: "22"; issue: "10"; pages: "1479-501"
COMMENT     Sequence Label: HA-mRIN2 dRHVPS9/PINCO
FEATURES             Location/Qualifiers
     source          1..14093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             332..801
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    866..1223
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1288..1704
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1634..1656
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1672..1688
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1714..2088
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2026..2042
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2100..2126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2106..2132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     enhancer        3905..4284
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4285..4488
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4439..4459
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4533..4552
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4533..4551
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4583..4592
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4589..4591,4592..4594,4595..5308)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4589..4591,4592..4594,4595..5305)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4634..4655)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4895..4914)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5242..5263
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5498..5967
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6097..6114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6268..6856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6348..6367)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6979..7113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7003..7022)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7057..7076
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7343..7942)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7426..7446)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7923..7942
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8025..8524)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8073..8092)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8466..8489
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      10640..12429
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(12923..13714,13715..13783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13546..13565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13784..13888)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgcaatattc ctgttaccaa agctagtata aataaaaata gataaacgtg gaaattactt
       61 agagtttctg tcattaacgt ttccttcctc agttgacaac ataaatgcgc tgctgagaag
      121 ccagtttgca tctgtcagga tcaatttccc attatgccag tcatattaat tactagtcaa
      181 ttagttgatt tttatttttg acatatacat gtgaaagacc ccacctgtag gtttggcaag
      241 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagaaaag
      301 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
      361 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
      421 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
      481 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
      541 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
      601 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc
      661 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
      721 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
      781 cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg
      841 gaccaccgac ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt
      901 gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta ctagttagct aactagctct
      961 gtatctggcg gacccgtggt ggaactgacg agttcggaac acccggccgc aaccctggga
     1021 gacgtcccag ggacttcggg ggccgttttt gtggcccgac ctgagtccaa aaatcccgat
     1081 cgttttggac tctttggtgc acccccctta gaggagggat atgtggttct ggtaggagac
     1141 gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg ggaccgaagc
     1201 cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt
     1261 ctgtatttgt ctgaaaatat gggcccgggc cagactgtta ccactccctt aagtttgacc
     1321 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
     1381 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
     1441 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
     1501 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
     1561 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
     1621 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
     1681 gccctcactc cttctctagg cgcccccata tggccatatg agatcttata tggggcaccc
     1741 ccgccccttg taaacttccc tgaccctgac atgacaagag ttactaacag cccctctctc
     1801 caagctcact tacaggctct ctacttagtc cagcacgaag tctggagacc tctggcggca
     1861 gcctaccaag aacaactgga ccgaccggtg gtacctcacc cttaccgagt cggcgacaca
     1921 gtgtgggtcc gccgacacca gactaagaac ctagaacctc gctggaaagg accttacaca
     1981 gtcctgctga ccacccccac cgccctcaaa gtagacggca tcgcagcttg gatacacgcc
     2041 gcccacgtga aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatccatgt
     2101 acccatacga tgttccggat tacgctagcc tcccagatct cgacccattt tgcattctca
     2161 gcatggtgag acacaaggat ggtggctact ctgaagacaa ggatgggaag acatgtcccc
     2221 gagactctgg ctatgacagc ctctccaaca ggctcagcat tctggaccgg ctcctgcata
     2281 cccatcccat atggctgcag ctgagtctga gtgaggagga ggcagcagag gtcctgcagg
     2341 cccagcctcc agggatcttt ttggttcgga aatcctccaa aatgcagaag aaagtcctct
     2401 cccttcgcct gccctgtgaa tttggagcac ctctcaaaga gtttactata aaggaaagca
     2461 cctacacctt ttccctggaa ggctcaggaa tcagctttgc agatttattc cggcttattg
     2521 ccttctactg catcagcagg gacgttttgc cattcacctt gaagctgcct tatgccattt
     2581 caacagccaa gacggagtct cagcttgaag aactagccca gctgggacta aatttttgga
     2641 gctcctcagc agacaacaaa cccccgaact ccccacctcc ccataggcct cttccctcag
     2701 ccggcatctg ccctgcctcc ttgcgtcagc tctgccttat aaatggagtg cattctataa
     2761 aaaccaggac gccttcggag ctggagtgca gccagaccaa cggagccttg tgttttatta
     2821 atcctctttt cttgaaagtg cacagccagg acctcagcac aggcccaaag cggcccagca
     2881 caaggactcc caacgcgaat ggcaccgaga gacctcggtc ccccccaccc aggcccccgc
     2941 cacccgctat taatagtctg cacacaagcc ctgggctatc caggactgaa ccccagacga
     3001 gcatgccaga aacagtcaac catagcaaac atgggaacgt agctctgctt ggaacaaaac
     3061 caactcccat ccctccaccc cggctgaaga agcaggcttc ttttttggaa gcagaaagca
     3121 gcgccaagac cttgactgcc cgccggccca gccggagctc ggagccggag cccgagctag
     3181 agctggagtt agagatgggc acagctgggc acgcaggtgg agccccgcct agggacgccc
     3241 ccggggattg cacaagggcg ccgccgcccg gctctgaatc acaaccacca ccgtgccatg
     3301 gagccaggca gaggctgagc gacatgagcc tttccacttc ctcctccgac tcgctggagt
     3361 tcgaccggag catgcctctg tatgggtacg aggccgacac caccagcagc ctggaggact
     3421 acgaagggga gagtgaccaa gagaccatgg cacctcccat caagtccaaa aagaagagga
     3481 acagctcttt tgtgctgccc aagcttagaa ccaccaaccg aactatcccc tccgttgacg
     3541 actttcagaa ttacctccga gttgccttcc aagaggtcaa cagcggatgc actgggaaga
     3601 ccctccttgt gagaccgtac atcaccacag aggatgtgtg tcagctctgt gctgagaagt
     3661 ttaaggtgga ggaccctgaa gagtacagcc tgtttctctt tgttgatgag acatggcaac
     3721 aactggcaga ggacacttat cctcagaaga tcaaggctga actgcacagc cggcctcagc
     3781 cccacatctt ccactttgtc tacaaacgaa tcaagagtga tccttacggg gtgattttcc
     3841 agaatggaga agacctcact ccctcatagg aattcgatcg atgtacgggc cagatatacg
     3901 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     3961 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     4021 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4081 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4141 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     4201 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     4261 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     4321 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     4381 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     4441 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
     4501 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
     4561 gcttcgaatt gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     4621 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     4681 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     4741 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     4801 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4861 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4921 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4981 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     5041 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     5101 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5161 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     5221 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     5281 tctcggcatg gacgagctgt acaagtaaag cggccgccag cacagtggtc gacgataaaa
     5341 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     5401 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
     5461 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
     5521 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
     5581 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     5641 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     5701 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     5761 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     5821 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     5881 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     5941 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt
     6001 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg
     6061 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6241 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6961 ccttttgcgg ccgaattaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     7021 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     7081 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     7141 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     7201 ggtatggctg attatgatcc tctagagtcg gtgggcctcg ggggcgggtg cggggtcggc
     7261 ggggccgccc cgggtggctt cggtcggagc catggggtcg tgcgctcctt tcggtcgggc
     7321 gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg
     7381 gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga
     7441 ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca
     7501 ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga
     7561 cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct
     7621 gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga
     7681 acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt
     7741 ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct
     7801 cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg
     7861 cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca
     7921 ccgtgggctt gtactcggtc atggaaggtc gtctccttgt gaggggtcag gggcgtgggt
     7981 caggggatgg tggcggcacc ggtcgtggcg gccgacctgc aggtcgaaag gcccggagat
     8041 gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct
     8101 ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac
     8161 ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg
     8221 ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg
     8281 agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac
     8341 ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt
     8401 tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa
     8461 gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct
     8521 acccggtaga attaattctc atgtttgaca gcttatcatc gataagctga tcctcacagg
     8581 ccgcacccag cttttcttcc gttgccccag tagcatctct gtctggtgac cttgaagagg
     8641 aagaggaggg gtcccgagaa tccccatccc taccgtccag caaaaagggg gacgaggaat
     8701 ttgaggcctg gcttgaggct caggacgcaa atcttgagga tgttcagcgg gagttttccg
     8761 ggctgcgagt aattggtgat gaggacgagg atggttcgga ggatggggaa ttttcagacc
     8821 tggatctgtc tgacagcgac catgaagggg atgagggtgg gggggctgtt ggagggggca
     8881 ggagtctgca ctccctgtat tcactgagcg tcgtctaata aagatgtcta ttgatctctt
     8941 ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac ctggaaatgg cctaggagag
     9001 aagggagaca catctggacc agaaggctcc ggcggcagtg gacctcaaag aagagggggt
     9061 gataaccatg gacgaggacg gggaagagga cgaggacgag gaggcggaag accaggagcc
     9121 ccgggcggct caggatcagg gccaagacat agagatggtg tccggagacc ccaaaaacgt
     9181 ccaagttgca ttggctgcaa agggacccac ggtggaacag gagcaggagc aggagcggga
     9241 ggggcaggag caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga
     9301 ggggsaggag caggaggggs aggagcagga ggggcaggag gggcaggagc aggaggaggg
     9361 gcaggagcag gaggggcagg agcaggaggt ggaggccggg gtcgaggagg cagtggaggc
     9421 cggggtcgag gaggtagtgg aggccggggt cgaggaggta gtggaggccg ccggggtaga
     9481 ggacgtgaaa gagccagggg gggaagtcgt gaaagagcca gggggagagg tcgtggacgt
     9541 ggagaaaaga ggcccaggag tcccagtagt cagtcatcat catccgggtc tccaccgcgc
     9601 aggccccctc caggtagaag gccatttttc caccctgtag gggaagccga ttattttgaa
     9661 taccaccaag aaggtggccc agatggtgag cctgacgtgc ccccgggagc gatagagcag
     9721 ggccccgcag atgacccagg agaaggccca agcactggac cccggggtca gggtgatgga
     9781 ggcaggcgca aaaaaggagg gtggtttgga aagcatcgtg gtcaaggagg ttccaacccg
     9841 aaatttgaga acattgcaga aggtttaaga gctctcctgg ctaggagtca cgtagaaagg
     9901 actaccgacg aaggaacttg ggtcgccggt gtgttcgtat atggaggtag taagacctcc
     9961 ctttacaacc taaggcgagg aactgccctt gctattccac aatgtcgtct tacaccattg
    10021 agtcgtctcc cctttggaat ggcccctgga cccggcccac aacctggccc gctaagggag
    10081 tccattgtct gttatttcat ggtcttttta caaactcata tatttgctga ggttttgaag
    10141 gatgcgatta aggaccttgt tatgacaaag cccgctccta cctgcaatat cagggtgact
    10201 gtgtgcagct ttgacgatgg agtagatttg cctccctggt ttccacctat ggtggaaggg
    10261 gctgccgcgg agggtgatga cggagatgac ggagatgaag gaggtgatgg agatgagggt
    10321 gaggaagggc aggagtgatg taacttgtta ggagacgccc tcaatcgtat taaaagccgt
    10381 gtattccccc gcactaaaga ataaatcccc agtagacatc atgcgtgctg ttggtgtatt
    10441 tctggccatc tgtcttgtca ccattttcgt cctcccaaca tggggcaatt gggcataccc
    10501 atgttgtcac gtcactcagc tccgcgctca acaccttctc gcgttggaaa acattagcga
    10561 catttacctg gtgagcaatc agacatgcga cggctttagc ctggcctcct taaattcacc
    10621 taagaatggg agcaaccagc aggaaaagga caagcagcga aaattcacgc ccccttggga
    10681 ggtggcggca tatgcaaagg atagcactcc cactctacta ctgggtatca tatgctgact
    10741 gtatatgcat gaggatagca tatgctaccc ggatacagat taggatagca tatactaccc
    10801 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
    10861 agatataaat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
    10921 agatatagat taggatagcc tatgctaccc agatatagat taggatagca tatgctaccc
    10981 agatatagat taggatagca tatgctatcc agatatttgg gtagtatatg ctacccagat
    11041 ataaattagg atagcatata ctaccctaat ctctattagg atagcatatg ctacccggat
    11101 acagattagg atagcatata ctacccagat atagattagg atagcatatg ctacccagat
    11161 atagattagg atagcctatg ctacccagat ataaattagg atagcatata ctacccagat
    11221 atagattagg atagcatatg ctacccagat atagattagg atagcctatg ctacccagat
    11281 atagattagg atagcatatg ctatccagat atttgggtag tatatgctac ccatggcaac
    11341 attagcccac cgtgctctca gcgacctcgt gaatatgagg accaacaacc ctgtgcttgg
    11401 cgctcaggcg caagtgtgtg taatttgtcc tccagatcgc agcaatcgcg cccctatctt
    11461 ggcccgccca cctacttatg caggtattcc ccggggtgcc attagtggtt ttgtgggcaa
    11521 gtggtttgac cgcagtggtt agcggggtta caatcagcca agttattaca cccttatttt
    11581 acagtccaaa accgcagggc ggcgtgtggg ggctgacgcg tgcccccact ccacaatttc
    11641 aaaaaaaaga gtggccactt gtctttgttt atgggcccca ttggcgtgga gccccgttta
    11701 attttcgggg gtgttagaga caaccagtgg agtccgctgc tgtcggcgtc cactctcttt
    11761 ccccttgtta caaatagagt gtaacaacat ggttcacctg tcttggtccc tgcctgggac
    11821 acatcttaat aaccccagta tcatattgca ctaggattat gtgttgccca tagccataaa
    11881 ttcgtgtgag atggacatcc agtctttacg gcttgtcccc accccatgga tttctattgt
    11941 taaagatatt cagaatgttt cattcctaca ctagtattta ttgcccaagg ggtttgtgag
    12001 ggttatattg gtgtcatagc acaatgccac cactgaaccc cccgtccaaa ttttattctg
    12061 ggggcgtcac ctgaaacctt gttttcgagc acctcacata caccttactg ttcacaactc
    12121 agcagttatt ctattagcta aacgaaggag aatgaagaag caggcgaaga ttcaggagag
    12181 ttcactgccc gctccttgat cttcagccac tgcccttgtg actaaaatgg ttcactaccc
    12241 tcgtggaatc ctgaccccat gtaaataaaa ccgtgacagc tcatggggtg ggagatatcg
    12301 ctgttcctta ggaccctttt actaacccta attcgatagc atatgcttcc cgttgggtaa
    12361 catatgctat tgaattaggg ttagtctgga tagtatatac tactacccgg gaagcatatg
    12421 ctacccgttt agggttaaca agggggcctt ataaacacta ttgctaatgc cctcttgagg
    12481 gtccgcttat cggtagctac acaggcccct ctgattgacg ttggtgtagc ctcccgtagt
    12541 cttcctgggc ccctgggagg tacatgtccc ccagcattgg tgtaagagct tcagccaaga
    12601 gttacacata aaggcaatgt tgtgttgcag tccacagact gcaaagtctg ctccaggatg
    12661 aaagccactc agtgttggca aatgtgcaca tccatttata aggatgtcaa ctacagtcag
    12721 agaacccctt tgtgtttggt ccccccccgt gtcacatgtg gaacagggcc cagttggcaa
    12781 gttgtaccaa ccaactgaag ggattacatg cactgccccg cgaagaaggg gcagagatgt
    12841 cgtagtcagg tttagttcgt ccggggcggg gatctggccg caaatcaatc taaagtatat
    12901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    12961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    13021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    13081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    13141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    13201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    13261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    13321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    13381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    13441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    13501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    13561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    13621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    13681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    13741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    13801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    13861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca ccagctttgc
    13921 tcttaggagt ttcctaatac atcccaaact caaatatata aagcatttga cttgttctat
    13981 gccctagggg gcggggggaa gctaagccag ctttttttaa catttaaaat gttaattcca
    14041 ttttaaatgc acagatgttt ttatttcata agggtttcaa tgtgcatgaa tgc
//
