LOCUS       sequence-210874-        1705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the 
            cytosol. Useful for detecting free zinc ion levels in the cytosol 
            (in vitro Kd ~2.3 nM, n = 0.53).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Fiedler BL, Van Buskirk S, Carter KP, Qin Y, Carpenter MC, Palmer 
            AE, Jimenez R
  TITLE     Droplet Microfluidic Flow Cytometer For Sorting On Transient 
            Cellular Responses Of Genetically-Encoded Sensors.
  JOURNAL   Anal Chem. 2017 Jan 3;89(1):711-719. doi: 
            10.1021/acs.analchem.6b03235. Epub 2016 Dec 13.
  PUBMED    27959493
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.analchem.6b03235"; journalName: "Anal Chem"; date: 
            "2017-01-3- 3"; volume: "89"; issue: "1"; pages: "711-719"
COMMENT     Sequence Label: pcDNA3.1(+)-NES-ZapCV2 (cpV143)
FEATURES             Location/Qualifiers
     source          1..1705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..46
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(56..58,59..61,62..574)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             962..1696
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 ggtaccgagc atgcttcaac ttcctcctct tgaacgtctt actctttcgg atcccatggt
       61 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      121 cgtaaacggc cacaggttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      181 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      241 gaccaccctg acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      301 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgtacca tcttcttcaa
      361 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      421 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      481 ggagtacaac tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
      541 caaggcccac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      601 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      661 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      721 ggagttcgtg accgccgccc gcatgcataa aaacaatgac ttaaaacaca aatggaaaga
      781 atgtcctgag tcttgtagct cactatttga cctacaaaga catcttttga aggatcatgt
      841 ctctcaagat ttcaaacacc ctatggaacc attagctcat aattgggagg actgtgattt
      901 ccttggagat gatacatgtt ccatagtgaa ccatattaat tgtcaacatg gtatcgagct
      961 catggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1021 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     1081 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1141 cggcatggac gagctgtaca agggtggcag cggtggcatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
     1381 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     1681 ccgccacaac atcgagtaag aattc
//
