LOCUS       sequence-210875-        1749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the 
            cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to 
            mutation of the zinc binding Cys residues to His..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Fiedler BL, Van Buskirk S, Carter KP, Qin Y, Carpenter MC, Palmer 
            AE, Jimenez R
  TITLE     Droplet Microfluidic Flow Cytometer For Sorting On Transient 
            Cellular Responses Of Genetically-Encoded Sensors.
  JOURNAL   Anal Chem. 2017 Jan 3;89(1):711-719. doi: 
            10.1021/acs.analchem.6b03235. Epub 2016 Dec 13.
  PUBMED    27959493
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.analchem.6b03235"; journalName: "Anal Chem"; date: 
            "2017-01-3- 3"; volume: "89"; issue: "1"; pages: "711-719"
COMMENT     Sequence Label: pb-NES-ZapCV5 (cpV143)
FEATURES             Location/Qualifiers
     source          1..1749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..63
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(73..75,76..78,79..591)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             979..1713
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 gaattcaact taagcttggg accgggcatg cttcaacttc ctcctcttga acgtcttact
       61 ctttcggatc cgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      121 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
      181 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      241 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      301 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      361 cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      421 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      481 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      541 aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcagc
      601 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      661 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
      721 gatcacatgg tcctgctgga gttcgtgacc gccgcccgca tgcataaaaa caatgactta
      781 aaacacaaat ggaaagaaca tcctgagtct tgtagctcac tatttgacct acaaagacat
      841 cttttgaagg atcatgtctc tcaagatttc aaacacccta tggaaccatt agctcataat
      901 tgggaggacc atgatttcct tggagatgat acatgttcca tagtgaacca tattaattgt
      961 caacatggta tcgagctcat ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     1021 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     1081 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1141 gccgccggga tcactctcgg catggacgag ctgtacaagg gtggcagcgg tggcatggtg
     1201 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1261 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1321 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1381 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1441 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1501 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1561 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1621 gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
     1681 aaggccaact tcaagatccg ccacaacatc gagtaagaat cctgcagata tccagcacag
     1741 tgggtcgac
//
