LOCUS       sequence-210882-       11993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FR cassette provider to introduce FR cassette to a BAC by 
            recombineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Sima J, Bartlett DA, Gordon MR, Gilbert DM
  TITLE     Bacterial artificial chromosomes establish replication timing and 
            sub-nuclear compartment de novo as extra-chromosomal vectors.
  JOURNAL   Nucleic Acids Res. 2018 Feb 28;46(4):1810-1820. doi: 
            10.1093/nar/gkx1265.
  PUBMED    29294101
REFERENCE   2  (bases 1 to 11993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-02-28- 28"; volume: "46"; issue: 
            "4"; pages: "1810-1820"
COMMENT     Sequence Label: pDY-HA1-HA2-neo
FEATURES             Location/Qualifiers
     source          1..11993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             385..2310
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGXGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     polyA_signal    5179..5227
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5252..5271
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5269..6306)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(6349..6494)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     promoter        complement(join(7835..7840,7841..7857,7858..7863))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     protein_bind    7892..7925
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(8158..8746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8238..8257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10072..10571
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10107..10130)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10504..10523
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        10583..10630
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10649..11452
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10712..10731)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11322..11341
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11484..11501)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11490..11714
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     11925..11943
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatcctcaca ggccgcaccc agcttttctt ccgttgcccc agtagcatct ctgtctggtg
       61 accttgaaga ggaagaggag gggtcccgag aatccccatc cctaccgtcc agcaaaaagg
      121 gggacgagga atttgaggcc tggcttgagg ctcaggacgc aaatcttgag gatgttcagc
      181 gggagttttc cgggctgcga gtaattggtg atgaggacga ggatggttcg gaggatgggg
      241 aattttcaga cctggatctg tctgacagcg accatgaagg ggatgagggt gggggggctg
      301 ttggaggggg caggagtctg cactccctgt attcactgag cgtcgtctaa taaagatgtc
      361 tattgatctc ttttagtgtg aatcatgtct gacgaggggc caggtacagg acctggaaat
      421 ggcctaggag agaagggaga cacatctgga ccagaaggct ccggcggcag tggacctcaa
      481 agaagagggg gtgataacca tggacgagga cggggaagag gacgaggacg aggaggcgga
      541 agaccaggag ccccgggcgg ctcaggatca gggccaagac atagagatgg tgtccggaga
      601 ccccaaaaac gtccaagttg cattggctgc aaagggaccc acggtggaac aggagcagga
      661 gcaggagcgg gaggggcagg agcaggaggg gcaggagcag gaggaggggc aggagcagga
      721 ggaggggcag gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca
      781 ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggagga
      841 ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc aggagcagga
      901 ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg
      961 gcaggagggg caggagcagg aggaggggca ggagcaggag gggcaggagg ggcaggaggg
     1021 gcaggagcag gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca
     1081 ggaggggcag gagcaggagg ggcaggagca ggaggggcag gagcaggagg ggcaggaggg
     1141 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
     1201 ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagc aggaggggca
     1261 ggaggggcag gagsaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga
     1321 gcaggaggag gggcaggagc aggaggggca ggagcaggag gtggaggccg gggtcgagga
     1381 ggcagtggag gccggggtcg aggaggtagt ggaggccggg gtcgaggagg tagtggaggc
     1441 cgccggggta gaggacgtga aagagccagg gggggaagtc gtgaaagagc cagggggaga
     1501 ggtcgtggac gtggagaaaa gaggcccagg agtcccagta gtcagtcatc atcatccggg
     1561 tctccaccgc gcaggccccc tccaggtaga aggccatttt tccaccctgt aggggaagcc
     1621 gattattttg aataccacca agaaggtggc ccagatggtg agcctgacgt gcccccggga
     1681 gcgatagagc agggccccgc agatgaccca ggagaaggcc caagcactgg accccggggt
     1741 cagggtgatg gaggcaggcg caaaaaagga gggtggtttg gaaagcatcg tggtcaagga
     1801 ggttccaacc cgaaatttga gaacattgca gaaggtttaa gagctctcct ggctaggagt
     1861 cacgtagaaa ggactaccga cgaaggaact tgggtcgccg gtgtgttcgt atatggaggt
     1921 agtaagacct ccctttacaa cctaaggcga ggaactgccc ttgctattcc acaatgtcgt
     1981 cttacaccat tgagtcgtct cccctttgga atggcccctg gacccggccc acaacctggc
     2041 ccgctaaggg agtccattgt ctgttatttc atggtctttt tacaaactca tatatttgct
     2101 gaggttttga aggatgcgat taaggacctt gttatgacaa agcccgctcc tacctgcaat
     2161 atcagggtga ctgtgtgcag ctttgacgat ggagtagatt tgcctccctg gtttccacct
     2221 atggtggaag gggctgccgc ggagggtgat gacggagatg acggagatga aggaggtgat
     2281 ggagatgagg gtgaggaagg gcaggagtga tgtaacttgt taggagacgc cctcaatcgt
     2341 attaaaagcc gtgtattccc ccgcactaaa gaataaatcc ccagtagaca tcatgcgtgc
     2401 tgttggtgta tttctggcca tctgtcttgt caccattttc gtcctcccaa catggggcaa
     2461 ttgggcatac ccatgttgtc acgtcactca gctccgcgct caacaccttc tcgcgttgga
     2521 aaacattagc gacatttacc tggtgagcaa tcagacatgc gacggcttta gcctggcctc
     2581 cttaaattca cctaagaatg ggagcaacca gcaggaaaag gacaagcagc gaaaattcac
     2641 gcccccttgg gaggtggcgg catatgcaaa ggatagcact cccactctac tactgggtat
     2701 catatgctga ctgtatatgc atgaggatag catatgctac ccggatacag attaggatag
     2761 catatactac ccagatatag attaggatag catatgctac ccagatatag attaggatag
     2821 cctatgctac ccagatataa attaggatag catatactac ccagatatag attaggatag
     2881 catatgctac ccagatatag attaggatag cctatgctac ccagatatag attaggatag
     2941 catatgctac ccagatatag attaggatag catatgctat ccagatattt gggtagtata
     3001 tgctacccag atataaatta ggatagcata tactacccta atctctatta ggatagcata
     3061 tgctacccgg atacagatta ggatagcata tactacccag atatagatta ggatagcata
     3121 tgctacccag atatagatta ggatagccta tgctacccag atataaatta ggatagcata
     3181 tactacccag atatagatta ggatagcata tgctacccag atatagatta ggatagccta
     3241 tgctacccag atatagatta ggatagcata tgctatccag atatttgggt agtatatgct
     3301 acccatggca acattagccc accgtgctct cagcgacctc gtgaatatga ggaccaacaa
     3361 ccctgtgctt ggcgctcagg cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg
     3421 cgcccctatc ttggcccgcc cacctactta tgcaggtatt ccccggggtg ccattagtgg
     3481 ttttgtgggc aagtggtttg accgcagtgg ttagcggggt tacaatcagc caagttatta
     3541 cacccttatt ttacagtcca aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca
     3601 ctccacaatt tcaaaaaaaa gagtggccac ttgtctttgt ttatgggccc cattggcgtg
     3661 gagccccgtt taattttcgg gggtgttaga gacaaccagt ggagtccgct gctgtcggcg
     3721 tccactctct ttccccttgt tacaaataga gtgtaacaac atggttcacc tgtcttggtc
     3781 cctgcctggg acacatctta ataaccccag tatcatattg cactaggatt atgtgttgcc
     3841 catagccata aattcgtgtg agatggacat ccagtcttta cggcttgtcc ccaccccatg
     3901 gatttctatt gttaaagata ttcagaatgt ttcattccta cactagtatt tattgcccaa
     3961 ggggtttgtg agggttatat tggtgtcata gcacaatgcc accactgaac cccccgtcca
     4021 aattttattc tgggggcgtc acctgaaacc ttgttttcga gcacctcaca tacaccttac
     4081 tgttcacaac tcagcagtta ttctattagc taaacgaagg agaatgaaga agcaggcgaa
     4141 gattcaggag agttcactgc ccgctccttg atcttcagcc actgcccttg tgactaaaat
     4201 ggttcactac cctcgtggaa tcctgacccc atgtaaataa aaccgtgaca gctcatgggg
     4261 tgggagataa caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt
     4321 atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg
     4381 gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca
     4441 taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac
     4501 tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc
     4561 tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc
     4621 aaccaactga agggattaca tgcactgccc cgcgaagaag gggcagagat gtcgtagtca
     4681 ggtttagttc gtccggggcg gggatcctct agagtcgacc tgcagcccaa gcttggcact
     4741 gggatcctct agagtcgacc tgcagcccaa gcttggcacg ccagaaatcc gcgcggtggt
     4801 ttttgggggt cgggggtgtt tggcagccac agacgcccgg tgttcgtgtc gcgccagtac
     4861 atgcggtcca tgcccaggcc atccaaaaac catgggtctg tctgctcagt ccagtcgtgg
     4921 acctgacccc acgcaacgcc caaaataata acccccacga accataaacc attccccatg
     4981 ggggaccccg tccctaaccc acggggccag tggctatggc agggcctgcc gccccgacgt
     5041 tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa
     5101 cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg
     5161 accgaacccc gcgtttatga acaaacgacc caacacccgt gcgttttatt ctgtcttttt
     5221 attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc agttagcctc
     5281 ccccatctcc cgatccggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
     5341 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
     5401 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
     5461 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
     5521 gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
     5581 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
     5641 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
     5701 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
     5761 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
     5821 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
     5881 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
     5941 gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag
     6001 gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa
     6061 ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
     6121 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
     6181 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
     6241 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
     6301 tttcatatct cattgccccc cgggatctgc ggcacgctgt tgacgctgtt aagcgggtcg
     6361 ctgcagggtc gctcggtgtt cgaggccaca cgcgtcacct taatatgcga agtggacctc
     6421 ggaccgcgcc gccccgactg catctgcgtg ttcgaattcg ccaatgacaa gacgctgggc
     6481 ggggtttgtg tcatcataga actaaagaca tgcaaatata tttcttccgg ggacaccgcc
     6541 agcaaacgcg agcaacgggc cacggggatg aagcagggcg gcacctcgct aacggattca
     6601 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc
     6661 cttggcagaa cataaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca
     6721 gccgcacgcg gcgcatctcg gggccgacgc gctgggctac gtcttgctgg cgttcgcgaa
     6781 gccaacacgg gaacagaaaa cggataccaa ggcgaagaat ctttatttaa caaagcgtac
     6841 tacggcggcg gcacgaactt cttccgtaaa gaaagccaga agcttcagca gagcgctaaa
     6901 aaacgcgatg ctgagttagc gaacggcgcc ctcggtatca tagagttaaa taatgattac
     6961 acattgaaaa aagtaatgaa gccgctgatc acttcaaaca cggtaactga tgaaatcgag
     7021 cgcgcgaatg ttttcaaaat gaacggcaaa tggtacttgt tcactgattc acgcggttca
     7081 aaaatgacga tcgatggtat taactcaaac gatatttaca tgcttggtta tgtatcaaac
     7141 tctttaaccg gcccttacaa gccgctgaac aaaacagggc ttgtgctgca aatgggtctt
     7201 gatccaaacg atgtgacatt cacttactct cacttcgcag tgccgcaagc caaaggcaac
     7261 aatgtggtta tcacaagcta catgacaaac agaggcttct tcgaggataa aaaggcaaca
     7321 tttgcgccaa gcttcttaat gaacatcaaa ggcaataaaa catccgttgt caaaaacagc
     7381 atcctggagc aaggacagct gacagtcaac taataacagc aaaaagaaaa tgccgatact
     7441 tcattggcat tttcttttat ttctcaacaa gatggtgaat tgactagtgg gtagatccac
     7501 aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc
     7561 aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat
     7621 tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg
     7681 acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
     7741 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
     7801 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt
     7861 caaacatgag aattgatccg gaacccttaa tataacttcg tataatgtat gctatacgaa
     7921 gttattaggt ccctcgacta tagggtcacc gtcgacagcg acacacttgc atcggatgca
     7981 gcccggttcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
     8041 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
     8101 acagcttcaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8161 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8221 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8281 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8341 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8401 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8461 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8521 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8581 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8641 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8701 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8761 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8821 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8881 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8941 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9001 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9061 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9121 cagaagtggt cctgcaactt tatccgcctc catccagtct attatgtcgc gactagtcaa
     9181 ttcgggagga tcgaaacggc agatcgcaaa aaacagtaca tacagaagga gacatgaaca
     9241 tgaacatcaa aaaaattgta aaacaagcca cagttctgac ttttacgact gcacttctgg
     9301 caggaggagc gactcaagcc ttcgcgaaag aaaataacca aaaagcatac aaagaaacgt
     9361 acggcgtctc tcatattaca cgccatgata tgctgcagat ccctaaacag cagcaaaacg
     9421 aaaaatacca agtgcctcaa ttcgatcaat caacgattaa aaatattgag tctgcaaaag
     9481 gacttgatgt gtgggacagc tggccgctgc aaaacgctga cggaacagta gctgaataca
     9541 acggctatca cgttgtgttt gctcttgcgg gaagcccgaa agacgctgat gacacatcaa
     9601 tctacatgtt ttatcaaaag gtcggcgaca actcaatcga cagctggaaa aacgcgggcc
     9661 gtgtctttaa agacagcgat aagttcgacg ccaacgatcc gatcctgaaa gatcagacgc
     9721 aagaatggtc cggttctgca acctttacat ctgacggaaa aatccgttta ttctacactg
     9781 actattccgg taaacattac ggcaaacaaa gcctgacaac agcgcaggta aatgtgtcaa
     9841 aatctgatga cacactcaaa atcaacggag tggaagatca caaaacgatt tttgacggag
     9901 acggaaaaac atatcagaac gttcagcagt ttatcgatga aggcaattat acatccggcg
     9961 acaaccatac gctgagagac cctcactacg ttgaagacag gtcgaggagg gtctgaagag
    10021 gagtttacgt ccagccaagc tagcttggct gcaggtcgtc gaaattctac cgggtagggg
    10081 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
    10141 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
    10201 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
    10261 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
    10321 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
    10381 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
    10441 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
    10501 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
    10561 cgggcctttc gacctgcagc ctgttgacaa ttaatcatcg gcatagtata tcggcatagt
    10621 ataatacgac aaggtgagga actaaaccat gggatcggcc attgaacaag atggattgca
    10681 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
    10741 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
    10801 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
    10861 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
    10921 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
    10981 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
    11041 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
    11101 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
    11161 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca
    11221 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
    11281 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
    11341 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
    11401 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatca
    11461 attctctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
    11521 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    11581 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
    11641 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
    11701 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcttgcgg
    11761 aaccctgtta ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11821 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11881 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    11941 ggccctttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gct
//
