LOCUS       sequence-210886-        6169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission:
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6169)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-CD151-7
FEATURES             Location/Qualifiers
     source          1..6169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        358..561
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     512..532
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             606..1283
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1332..2006
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     polyA_signal    2950..3071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2987..3006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3041..3060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3078..3533)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3215..3236)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3427..3446
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3560..3664
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3666..4023
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3666..3857
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3689..3709)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3874..4009
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4058..4852
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4058..4852
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4112..4131)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4722..4741
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5040..5059)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5084..5131
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5460..6048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5949..5968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
       61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg
      601 ccaccatgag tgcgattaag ccagacatga agatcaacct ccgtatggaa ggcaacgtaa
      661 acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga
      721 gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca
      781 ctgcattcca ttacggcaac agggtattcg ccgaatatcc agaccacata caagactatt
      841 ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg
      901 gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc
      961 gatttcacgg tgtaaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat
     1021 gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgactggt gatattcgca
     1081 tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag
     1141 ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc attgagattt
     1201 taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg
     1261 gattgcctga caatgccaga cgatccggta gcggtcacgg tactggttct actggttctg
     1321 gttcttctga gagtgcgatt aagccagaca tgaagatcaa cctccgtatg gaaggcaacg
     1381 taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac
     1441 agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga
     1501 ccactgcatt ccattacggc aacagggtat tcgccgaata tccagaccac atacaagact
     1561 attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg
     1621 ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc tataataaag
     1681 ttcgatttca cggtgtaaac tttcccgcca atggtccagt tatgcagaag aagacgctga
     1741 aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgact ggtgatattc
     1801 gcatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca
     1861 aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga
     1921 ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt
     1981 ctggattgcc tgacaatgcc agacgatccg gactcagatc tggcggtatg ggtgagttca
     2041 acgagaagaa gacaacatgt ggcaccgttt gcctcaagta cctgctgttt acctacaatt
     2101 gctgcttctg gctggctggc ctggctgtca tggcagtggg catctggacg ctggccctca
     2161 agagtgacta catcagcctg ctggcctcag gcacctacct ggccacagcc tacatcctgg
     2221 tggtggcggg cactgtcgtc atggtgactg gggtcttggg ctgctgcgcc accttcaagg
     2281 agcgtcggaa cctgctgcgc ctgtacttca tcctgctcct catcatcttt ctgctggaga
     2341 tcatcgctgg tatcctcgcc tacgcctact accagcagct gaacacggag ctcaaggaga
     2401 acctgaagga caccatgacc aagcgctacc accagccggg ccatgaggct gtgaccagcg
     2461 ctgtggacca gctgcagcag gagttccact gctgtggcag caacaactca caggactggc
     2521 gagacagtga gtggatccgc tcacaggagg ccggtggccg tgtggtccca gacagctgct
     2581 gcaagacggt ggtggctctt tgtggacagc gagaccatgc ctccaacatc tacaaggtgg
     2641 agggcggctg catcaccaag ttggagacct tcatccagga gcacctgagg gtcattgggg
     2701 ctgtggggat cggcattgcc tgtgtgcagg tctttggcat gatcttcacg tgctgcctgt
     2761 acaggagtct caagctggag cactactgag aattctgcag tcgacggtac cgcgggcccg
     2821 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     2881 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2941 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3001 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3061 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3121 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3181 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3241 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3301 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3361 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3421 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3481 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     3541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3661 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     3721 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     3781 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3841 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3901 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3961 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4021 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4081 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4141 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4201 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     4261 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4321 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4381 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4441 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4501 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4561 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     4621 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     4681 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     4741 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     4801 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     4861 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     4921 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     4981 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     5041 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     5101 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     5161 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     5221 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     5281 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     5341 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5401 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5461 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5521 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5581 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5641 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5701 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5761 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5821 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5881 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5941 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6001 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6061 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6121 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttat
//
