LOCUS       sequence-210887-        3127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contain promoter PthrC, which is used for study about 
            Short, Auto-inducible Promoters for Well-Controlled Protein 
            Expression in Escherichia coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Anilionyte O, Liang H, Ma X, Yang L, Zhou K
  TITLE     Short, auto-inducible promoters for well-controlled protein 
            expression in Escherichia coli.
  JOURNAL   Appl Microbiol Biotechnol. 2018 Aug;102(16):7007-7015. doi: 
            10.1007/s00253-018-9141-z. Epub 2018 Jun 8.
  PUBMED    29948110
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-018-9141-z"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2018-08"; volume: "102"; issue: "16"; pages: 
            "7007-7015"
COMMENT     Sequence Label: pMB1-spect-PthrC-eGFP
FEATURES             Location/Qualifiers
     source          1..3127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     34..51
                     /label=L4440
                     /note="L4440 vector, forward primer"
     RBS             330..352
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(360..362,363..1076)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(360..362,363..1073)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(402..423)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(663..682)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1010..1031
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1437..2228
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2419..3007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2908..2927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
       61 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgc
      121 tggatccttc cctcgactca cacttgggag cttttcattc tgactgcaac gggcaatatg
      181 tctctgtgtg gattaaaaaa agagtgtctg atagcagctt ctgaactggt tacctgccgt
      241 gagtaaatta aaattttatt gacttaggtc actaaatact ttaaccaata taggcatagc
      301 gcacagacag ataaaaattt agaaataatt ttgtttaact ttaagaagga gatatacata
      361 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      421 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      481 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      541 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      601 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      661 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      721 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      781 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
      841 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
      901 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
      961 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1021 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtgatggg
     1081 ctgaagggtt taagcgaaga cgaacaataa ggcctcccaa atcggggggc cttttttatt
     1141 gataacaaaa aggcaacact atgacatcgg aaaacccgtt actggcgctg cgagagaaaa
     1201 tcagcgcgct ggatgaaaaa ttattagcgt tactggcaga gcggcgcgaa ttcaaacaga
     1261 aaggccatgg gtcgacctgc agaagcttag atctattacc ctgttatccc tactcgagtt
     1321 catgtgcagc tccataagca aaaggggatg ataagtttat caccaccgac tatttgcaac
     1381 agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcatga
     1441 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     1501 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     1561 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     1621 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     1681 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     1741 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     1801 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     1861 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     1921 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     1981 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     2041 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     2101 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     2161 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     2221 gcaaataaga tgccgctcgc cagtcgattg gctgagctca taagttccta ttccgaagtt
     2281 ccgcgaacgc gtaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2341 cttaacgtca cacaacatag ccacggagtt ttcgttccac tgagcgtcag accccgtaga
     2401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2521 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     2581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3061 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3121 gtgagct
//
