LOCUS       sequence-210891-        3291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contain promoter PthrC3, which is used for study about 
            Short, Auto-inducible Promoters for Well-Controlled Protein 
            Expression in Escherichia coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Anilionyte O, Liang H, Ma X, Yang L, Zhou K
  TITLE     Short, auto-inducible promoters for well-controlled protein 
            expression in Escherichia coli.
  JOURNAL   Appl Microbiol Biotechnol. 2018 Aug;102(16):7007-7015. doi: 
            10.1007/s00253-018-9141-z. Epub 2018 Jun 8.
  PUBMED    29948110
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-018-9141-z"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2018-08"; volume: "102"; issue: "16"; pages: 
            "7007-7015"
COMMENT     Sequence Label: pMB1-spect-PthrC3-eGFP
FEATURES             Location/Qualifiers
     source          1..3291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     114..131
                     /label=L4440
                     /note="L4440 vector, forward primer"
     RBS             574..596
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(604..606,607..1320)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(604..606,607..1317)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(646..667)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(907..926)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1254..1275
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1681..2472
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2663..3251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3152..3171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
       61 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      121 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
      181 gtatttcaca ccgcatatgc tggatccttc cctcgactca cacttgggag cttttcattc
      241 tgactgcaac gggcaatatg tctctgtgtg gattaaaaaa agagtgtctg atagcagctt
      301 ctgaactggt tacctgccgt gagtaaatta aaattttatt gacttaggtc actaaatact
      361 ttaaccaata taggcatagc gcacagacag ataaaaatta cagagtacac aacatccatg
      421 aaacgcatta gcaccaccat taccaccacc atcaccatta ccacaggtaa cggtgcgggc
      481 tgacgcgtac aggaaacaca gaaaaaagcc cgcacctgac agtgcgggct tttttttcga
      541 ccaaaggtaa cgaggtaaca acctagaaat aattttgttt aactttaaga aggagatata
      601 catatgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      661 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      721 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      781 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      841 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      901 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      961 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1021 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     1081 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1141 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1201 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1261 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga
     1321 tgggctgaag ggtttaagcg aagacgaaca ataaggcctc ccaaatcggg gggccttttt
     1381 tattgataac aaaaaggcaa cactatgaca tcggaaaacc cgttactggc gctgcgagag
     1441 aaaatcagcg cgctggatga aaaattatta gcgttactgg cagagcggcg cgaattcaaa
     1501 cagaaaggcc atgggtcgac ctgcagaagc ttagatctat taccctgtta tccctactcg
     1561 agttcatgtg cagctccata agcaaaaggg gatgataagt ttatcaccac cgactatttg
     1621 caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc
     1681 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     1741 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     1801 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     1861 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     1921 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     1981 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     2041 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     2101 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     2161 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     2221 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     2281 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     2341 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     2401 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     2461 gtcggcaaat aagatgccgc tcgccagtcg attggctgag ctcataagtt cctattccga
     2521 agttccgcga acgcgtaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2581 atcccttaac gtcacacaac atagccacgg agttttcgtt ccactgagcg tcagaccccg
     2641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt t
//
