LOCUS       sequence-210892-        3291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contain promoter PthrC3_8, which is used for study 
            about Short, Auto-inducible Promoters for Well-Controlled Protein 
            Expression in Escherichia coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Anilionyte O, Liang H, Ma X, Yang L, Zhou K
  TITLE     Short, auto-inducible promoters for well-controlled protein 
            expression in Escherichia coli.
  JOURNAL   Appl Microbiol Biotechnol. 2018 Aug;102(16):7007-7015. doi: 
            10.1007/s00253-018-9141-z. Epub 2018 Jun 8.
  PUBMED    29948110
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-018-9141-z"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2018-08"; volume: "102"; issue: "16"; pages: 
            "7007-7015"
COMMENT     Sequence Label: pMB1-spect-PthrC3_8-eGFP
FEATURES             Location/Qualifiers
     source          1..3291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..149
                     /label=L4440
                     /note="L4440 vector, forward primer"
     RBS             592..614
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(622..624,625..1338)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(622..624,625..1335)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(664..685)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(925..944)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1272..1293
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1699..2490
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2681..3269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3170..3189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
       61 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
      181 tccttacgca tctgtgcggt atttcacacc gcatatgctg gatccttccc tcgactcaca
      241 cttgggagct tttcattctg actgcaacgg gcaatatgtc tctgtgtgga ttaaaaaaag
      301 agtgtctgat agcagcttct gaactggtta cctgccgtga gtaaattaaa attttattga
      361 cttaggtcac taaatacttt aaccaatata ggcatagcgc acagacagat aaaaattaca
      421 gagtacacaa catccatgaa acgcattagc accaccatta ccaccaccat caccattacc
      481 acaggtaaag gtgcgggctg tcgcgtacag gaaacacaga aaaaagcccg cacctgacag
      541 tgcgggcttt tttttcgacc aaaggtaacg aggtaacaac ctagaaataa ttttgtttaa
      601 ctttaagaag gagatataca tatgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      661 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      721 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      781 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
      841 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      901 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      961 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1021 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1081 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1141 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1201 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     1261 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1321 gacgagctgt acaagtgatg ggctgaaggg tttaagcgaa gacgaacaat aaggcctccc
     1381 aaatcggggg gcctttttta ttgataacaa aaaggcaaca ctatgacatc ggaaaacccg
     1441 ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc gttactggca
     1501 gagcggcgcg aattcaaaca gaaaggccat gggtcgacct gcagaagctt agatctatta
     1561 ccctgttatc cctactcgag ttcatgtgca gctccataag caaaagggga tgataagttt
     1621 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
     1681 gcggtggagt gcaatgtcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     1741 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     1801 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     1861 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     1921 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     1981 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     2041 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     2101 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     2161 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     2221 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     2281 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     2341 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     2401 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     2461 ggcgagatca ccaaggtagt cggcaaataa gatgccgctc gccagtcgat tggctgagct
     2521 cataagttcc tattccgaag ttccgcgaac gcgtaaagga tctaggtgaa gatccttttt
     2581 gataatctca tgaccaaaat cccttaacgt cacacaacat agccacggag ttttcgttcc
     2641 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2701 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2761 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2821 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2881 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2941 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3001 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3061 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt t
//
