LOCUS       sequence-210905-        3182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcUBI promoter module Pro+5U.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3182)
  TITLE     Chevalier Lab plasmids
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pORFE0011
FEATURES             Location/Qualifiers
     source          1..3182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      260..848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     749..768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1002..1019
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2070..2088)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2156..2260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2480..3182,1..89)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat tacgtaaaag
       61 gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca tatatacttt
      121 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
      181 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      241 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      301 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      361 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
      421 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      481 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      541 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      601 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      661 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      721 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      781 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      841 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      901 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      961 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1021 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1081 cactctgtgg tctcaggagc tagcaacgat tgtacaattg cttctttaaa aaaggaagaa
     1141 agaaagaaag aaaagaatca acatcagcgt taacaaacgg ccccgttacg gcccaaacgg
     1201 tcatatagag taacggcgtt aagcgttgaa agactcctat cgaaatacgt aaccgcaaac
     1261 gtgtcatagt cagatcccct cttccttcac cgcctcaaac acaaaaataa tcttctacag
     1321 cctatatata caaccccccc ttctatctct cctttctcac aattcatcat ctttctttct
     1381 ctacccccaa ttttaagaaa tcctctcttc tcctcttcat tttcaaggta aatctctctc
     1441 tctctctctc tctctgttat tccttgtttt aattaggtat gtattattgc tagtttgtta
     1501 atctgcttat cttatgtatg ccttatgtga atatctttat cttgttcatc tcatccgttt
     1561 agaagctata aatttgttga tttgactgtg tatctacacg tggttatgtt tatatctaat
     1621 cagatatgaa tttcttcata ttgttgcgtt tgtgtgtacc aatccgaaat cgttgatttt
     1681 tttcatttaa tcgtgtagct aattgtacgt atacatatgg atctacgtat caattgttca
     1741 tctgtttgtg tttgtatgta tacagatctg aaaacatcac ttctctcatc tgattgtgtt
     1801 gttacataca tagatataga tctgttatat catttttttt attaattgtg tatatatata
     1861 tgtgcataga tctggattac atgattgtga ttatttacat gattttgtta tttacgtatg
     1921 tatatatgta gatctggact ttttggagtt gttgacttga ttgtatttgt gtgtgtatat
     1981 gtgtgttctg atcttgatat gttatgtatg tgcagcgaat tcggcgcgca aatgtgagac
     2041 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2101 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2161 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2221 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     2281 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     2341 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     2401 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2461 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     2521 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     2581 cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt
     2641 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     2701 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     2761 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     2821 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     2881 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     2941 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     3001 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     3061 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     3121 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     3181 ag
//
