LOCUS       sequence-210922-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express shRNA targeting SHMT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Minton DR, Nam M, McLaughlin DJ, Shin J, Bayraktar EC, Alvarez SW, 
            Sviderskiy VO, Papagiannakopoulos T, Sabatini DM, Birsoy K, 
            Possemato R
  TITLE     Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial 
            Translation Initiation and Maintenance of Formylmethionyl-tRNAs.
  JOURNAL   Mol Cell. 2018 Feb 15;69(4):610-621.e5. doi: 
            10.1016/j.molcel.2018.01.024.
  PUBMED    29452640
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.01"; journalName: "Mol Cell"; date: 
            "2018-02-15- 15"; volume: "69"; issue: "4"; pages: "610-621.e5"
COMMENT     Sequence Label: pLKO.1P shSHMT2
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             187..420
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    492..613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(529..548)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     583..602
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     715..734
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
       61 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcggtac
      121 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
      181 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
      241 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      301 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      361 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      421 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
      481 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
      541 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
      601 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
      661 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
      721 tgcagaggcc gagg
//
